GSK-3 beta [p Tyr216] Antibody

Images

 
Western Blot: GSK-3 beta [p Tyr216] Antibody [NB100-81946] - Analysis of extracts from 293 cells untreated or treated with PMA using GSK-3 beta (P-Tyr216) antibody.
Immunocytochemistry/ Immunofluorescence: GSK-3 beta [p Tyr216] Antibody [NB100-81946] - Methanol-fixed MEF cells.
Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GSK-3 beta (PTyr216) antibody.
Immunocytochemistry/ Immunofluorescence: GSK-3 beta [p Tyr216] Antibody [NB100-81946] - Staining of Methanol-fixed Hela cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GSK-3 beta [p Tyr216] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human GSK3 beta around the phosphorylation site of tyrosine 216 (V-S-YP-I-C).
Modification
p Tyr216
Specificity
GSK3 beta (specific to Phospho-Tyr216) detects endogenous levels of GSK3 beta only when phosphorylated at tyrosine 216.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSK3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
GSK-3 beta Lysate (NBL1-11362)
Publications
Read Publication using
NB100-81946 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GSK-3 beta [p Tyr216] Antibody

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GSK-3 beta Antibody (NB100-81946)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Human
(1)
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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSK3B
Entrez
Uniprot