GSK-3 beta [p Tyr216] Antibody

Images

 
Western Blot: GSK-3 beta [p Tyr216] Antibody [NB100-81946] - Analysis of extracts of various cell lines,using GSK-3 beta (Phospho-Tyr279/216) Antibody
Immunocytochemistry/ Immunofluorescence: GSK-3 beta [p Tyr216] Antibody [NB100-81946] - Methanol-fixed MEF cells.
Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GSK-3 beta (PTyr216) antibody.
Western Blot: GSK-3 beta [p Tyr216] Antibody [NB100-81946] - Analysis of extracts from 293 cells untreated or treated with PMA using GSK-3 beta (P-Tyr216) antibody.
Immunocytochemistry/ Immunofluorescence: GSK-3 beta [p Tyr216] Antibody [NB100-81946] - Staining of Methanol-fixed Hela cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GSK-3 beta [p Tyr216] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human GSK3 beta around the phosphorylation site of tyrosine 216 (V-S-YP-I-C).
Modification
p Tyr216
Specificity
GSK3 beta (specific to Phospho-Tyr216) detects endogenous levels of GSK3 beta only when phosphorylated at tyrosine 216.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSK3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
GSK-3 beta Overexpression Lysate
Publications
Read Publications using
NB100-81946 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • WB
    3 publications

Reactivity Notes

Use in Mammal reported in scientific literature (PMID:33413691)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GSK-3 beta [p Tyr216] Antibody

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GSK-3 beta Antibody (NB100-81946)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Mammal.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(2)
WB
(3)
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Human
(2)
Mouse
(4)
Mammal
(1)
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Showing Publications 1 - 7 of 7.
Publications using NB100-81946 Applications Species
Takaichi Y, Chambers JK, Takahashi K, et al. Amyloid beta and tau pathology in brains of aged pinniped species (sea lion, seal, and walrus) Acta neuropathologica communications Jan 7 2021 [PMID: 33413691] (IHC-P, Mammal) IHC-P Mammal
Wu L, Lin W, Liao Q et al. Calcium Channel Blocker Nifedipine Suppresses Colorectal Cancer Progression and Immune Escape by Preventing NFAT2 Nuclear Translocation Cell Rep Oct 27 2020 [PMID: 33113363] (WB, Human) WB Human
Liu Z, Selby CP, Yang Y et al. Circadian regulation of c-MYC in mice Proc. Natl. Acad. Sci. U.S.A. Aug 19 2020 [PMID: 32817420] (WB, Mouse) WB Mouse
Takaichi Y, Chambers JK, Inoue H et al. Phosphorylation and oligomerization of alpha-synuclein associated with GSK-3 beta activation in the rTg4510 mouse model of tauopathy Acta Neuropathol Commun Jun 19 2020 [PMID: 32560668] (IHC, Mouse) IHC Mouse
Wu X, Kosaraju J, Tam kY. Anti-neuroinflammatory effects of SLOH in AB-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSk-3B. Neurosci. Lett. Sep 29 2018 [PMID: 30278248] (WB, Mouse) WB Mouse
Wu X, Kosaraju J, Zhou W, Tam KY. SLOH, a carbazole-based fluorophore, mitigates neuropathology and behavioral impairment in the triple-transgenic mouse model of Alzheimer's disease Neuropharmacology 2018 Jan 05 [PMID: 29309769] (Mouse) Mouse
Denysenko T, Annovazzi L, Cassoni P et al. WNT/b-catenin Signaling Pathway and Downstream Modulators in Low- and High-grade Glioma. Cancer Genomics Proteomics. 2015 Dec 28 [PMID: 26708597] (IHC-P, Human) IHC-P Human

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol GSK3B
Entrez
Uniprot