Nitrotyrosine Antibody (39B6)

Images

 
Immunocytochemistry/ Immunofluorescence: Nitrotyrosine Antibody (39B6) [NB110-96877] - Analysis of Mouse Retinal Injury Model using Nitrotyrosine Antibody (SMC-154). This image is courtesy of Dr. Rajashekhar Gangaraju, ...read more
Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at ...read more
Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Analysis using Rat Anti-Nitrotyrosine Monoclonal Antibody. Tissue: colon carcinoma. Fixation: Formalin. Primary Antibody: Rat ...read more
Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary ...read more
Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: liver tissue. Species: Rat. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
39B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Nitrotyrosine Antibody (39B6) Summary

Immunogen
Hybridoma line 39B6
Specificity
Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.7 ug/ml
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
0.7 ug/ml of Nitrotyrosine Antibody was sufficient for detection of 5 ug SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Publications
Read Publications using
NB110-96877 in the following applications:

Reactivity Notes

Species Independent

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Nitrotyrosine Antibody (39B6)

  • Nitrotyrosine

Background

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nitrotyrosine Antibody (NB110-96877)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nitrotyrosine Antibody (NB110-96877) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Nitrotyrosine Products

Nitrotyrosine NB110-96877

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