Nitrotyrosine Antibody (39B6)


Immunocytochemistry/ Immunofluorescence: Nitrotyrosine Antibody (39B6) [NB110-96877] - Analysis of Mouse Retinal Injury Model using Nitrotyrosine Antibody (SMC-154). This image is courtesy of Dr. Rajashekhar Gangaraju, more
Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at more
Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Analysis using Rat Anti-Nitrotyrosine Monoclonal Antibody. Tissue: colon carcinoma. Fixation: Formalin. Primary Antibody: Rat more
Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary more
Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: liver tissue. Species: Rat. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat more

Product Details

Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

Order Details

Nitrotyrosine Antibody (39B6) Summary

3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.
Recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific.
Protein G purified
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS and 50% Glycerol
0.09% Sodium Azide
1.0 mg/ml
Protein G purified


  • Western Blot 0.7 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Read Publications using
NB110-96877 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Nitrotyrosine Antibody (39B6)

  • Nitrotyrosine


Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nitrotyrosine Antibody (NB110-96877)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, IHC-P.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Additional Nitrotyrosine Antibody (39B6) Products

Nitrotyrosine NB110-96877

Blogs on Nitrotyrosine

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