| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24861 | |||
| BSA Free | NB100-56720SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48337SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56581SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48338SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24865 | |||
| Azide and BSA Free | NBP2-24865SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27369SS | |||
| Azide and BSA Free | NBP2-80862 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56334SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56404SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-35334SS | |||
| Azide and BSA Free | NBP2-80623 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24821SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27363 | |||
| BSA Free | NBP2-27362SS | |||
| Azide and BSA Free | NBP2-27363-0.5mg | |||
| Azide and BSA Free | NBP2-27363-1mg | |||
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels of TLR3 in sham and MI hearts. n = 4 mice/group. Data are means +/- S.D. AP < 0.01 versus sham. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR3-Antibody-Western-Blot-NB100-56571-img0009.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) and (B) show mRNA and protein levels of TLR3 in sham and MI hearts. n = 4 mice/group. Data are means +/- S.D. AP < 0.01 versus sham. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Frozen: TLR3 Antibody [NB100-56571] - Frozen mouse eye tissue sections stained with TLR3, a retinal pigment epithelium (RPE) marker and DAPI. Top panel: TLR3 (red) colocalized with the RPE (green) marker. Bottom panel: Tissue stained with isotype control (no TLR3 Ab), RPE and DAPI. Data courtesy of Kleinman et al.](http://images.novusbio.com/fullsize/TLR3-Antibody-Immunohistochemistry-Frozen-NB100-56571-img0002.jpg)
![Flow Cytometry: TLR3 Antibody [NB100-56571] - Analysis using the FITC conjugate of NB100-56571. Staining of TLR3 in Balb/c mouse splenocytes using this antibody at 1 ug/10^6 cells. Green represents rabbit IgG isotype control this antibody; red represents anti-TLR3 antibody.](http://images.novusbio.com/fullsize/TLR3-Antibody-Flow-Cytometry-NB100-56571-img0008.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Analysis of TLR3 in mouse spleen tissue lysate using NB100-56571 at 2 ug/ml.](http://images.novusbio.com/fullsize/TLR3-Antibody-Western-Blot-NB100-56571-img0005.jpg)
![Immunohistochemistry: TLR3 Antibody [NB100-56571] - Pig lung poisoned through aspiration with gastric juice and organophosphate (dimethoate EC40) after 48 hours of IC ventilation. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR3-Antibody-Immunohistochemistry-NB100-56571-img0006.jpg)
![Flow Cytometry: TLR3 Antibody [NB100-56571] - Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes and mouse RAW cells using TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-Flow-Cytometry-NB100-56571-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TLR3 Antibody [NB100-56571] - siRNA was transfected at 50 nM for 48 hrs, and poly(I:C) was added at 100 ug/ml for 4 hrs before cell harvest. Negative control (NC) siRNA served as control. RNAiMAX transfection reagent was used in all the siRNA experiments. Panel shows the knockdown effects of siRNAs. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13328) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR3-Antibody-Knockdown-Validated-NB100-56571-img0010.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415523938.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-3102024165731.jpg)
![Immunohistochemistry: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression & its physical association with Trif in mouse myocardium. Heart samples were taken from infarct area at 4 weeks after MI. (A) & (B) show mRNA & protein levels of TLR3 in sham & MI hearts. n = 4 mice/group. Data are means ± S.D. AP < 0.01 versus sham. (C) Representative immunohistochemistry images of heart sections stained for TLR3 (brown colour). An isotype IgG control was performed to verify the specificity of TLR3 reactivity. (D) Lysates of heart tissue were immunoprecipitated with anti‐TLR3 antibodies (IP: TLR3), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG (IP: IgG) was performed as a control to exclude the non‐specific binding of antibodies to cellular proteins. Green arrows indicate non‐specific bands. The association between TLR3 & Trif, but not MyD88, was detectable in sham myocardium & was increased in infarct myocardium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415535158.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - TLR3‐knockout (TLR3‐KO) attenuated cardiac autophagy induced by MI. The infarct & remote tissues were separately sampled from the left ventricle after 4 weeks of MI. Accordingly, anterior & posterior tissues of the left ventricle were sampled from sham hearts as controls. (A) Representative Western blot images & quantitative analyses of autophagy markers. n = 4–8/group. Quantitative data are fold changes of WT‐sham. aP < 0.05, AP < 0.01 versus respective WT‐sham; bP < 0.05, BP < 0.01 versus respective WT‐MI. (B) Representative electron microphotographs of ultrathin sections of resin‐embedded heart biopsies. Arrows indicate autophagic vacuoles. (C) Lysates of infarct tissue were immunoprecipitated (IP), followed by SDS–PAGE & immunoblotting (IB) with indicated antibodies. IP with isotype IgG served as a control. Green arrows indicate non‐specific bands. Representative images were taken from four independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-310202415525214.jpg)
![Western Blot: TLR3 Antibody [NB100-56571] - A TLR3 agonist polyinosinic‐polycytidylic acid (poly(I:C)) induced autophagy in cultured cardiomyocytes through a TRIF‐dependent pathway. (A) Poly(I:C) increased autophagy markers in cultured H9c2 rat ventricular cells. (B) Poly(I:C) stimulated autophagosome formation but did not affect autophagic flux. Primary cultured neonatal rat ventricular myocytes (NRVMs) were transfected with a tandem mRFP‐GFP‐LC3 adenovirus for 24 hrs, followed by treatment with poly(I:C) (100 μg/ml, 4 hrs). Autophagosomes & autolysosomes were, respectively, visualized as yellow‐ & red‐only punctas under a confocal microscope. (C) An autophagic flux inhibitor chloroquine (CQ) induced accumulations of LC3‐II & p62/SQSTM1 proteins in H9c2 myocytes receiving poly(I:C) (100 μg/ml, 4 hrs). CQ was applied at 10 μM, immediately prior to poly(I:C). (D) Effects of indicated siRNA on poly(I:C)‐induced changes in autophagy markers in NRVMs. All the siRNAs were transfected at 50 nM for 48 hrs, & poly(I:C) was added at 100 μg/ml for 4 hrs before cell harvest. Negative control (NC) siRNA served as control. RNAiMAX transfection reagent was used in all the siRNA experiments. The upper panel shows the knockdown effects of siRNAs, & the lower panel shows representative Western blot images (presented from four independent experiments) & densitometry quantitative data (normalized into ‘fold of vehicle group’). All quantitative data are expressed as means ± S.D. aP < 0.05, AP < 0.01 versus vehicle; bP < 0.05, BP < 0.01 versus poly(I:C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28945004), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56571_rabbit-polyclonal-tlr3-antibody-3102024168394.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TLR2 Antibody [NB100-56720] - Analysis using the Azide Free version of NB100-56720. Detection of TLR2 in transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-Western-Blot-NB100-56720-img0005.jpg)
![Immunohistochemistry-Paraffin: TLR2 Antibody [NB100-56720] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-Immunohistochemistry-Paraffin-NB100-56720-img0003.jpg)
![Immunohistochemistry: CCR2 Antibody [NBP1-48337] - Localization of CCR1 (binds to CCL8, CCL14, and CCL16), CCR2 (binds to CCL2, CCL8, and CCL16), CCR3 (binds to CCL11), and CXCR3 (binds to CXCL10) in the bovine endometrium and fetal trophoblast obtained from cows in their 18th day of pregnancy. Intensive immunoreactivity was observed in endometrial epithelial cells, glandular epithelial cells, or fetal trophoblast. No positive immunoreactivity was observed in the negative control (Control). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/18/4/742), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-NBP1-48337-img0017.jpg "Immunohistochemistry: CCR2 Antibody [NBP1-48337] - Localization of CCR1 (binds to CCL8, CCL14, and CCL16), CCR2 (binds to CCL2, CCL8, and CCL16), CCR3 (binds to CCL11), and CXCR3 (binds to CXCL10) in the bovine endometrium and fetal trophoblast obtained from cows in their 18th day of pregnancy. Intensive immunoreactivity was observed in endometrial epithelial cells, glandular epithelial cells, or fetal trophoblast. No positive immunoreactivity was observed in the negative control (Control). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/18/4/742), licensed under a CC-BY license.")
![Western Blot: CCR2 Antibody [NBP1-48337] - Analysis of CCR2 in rat lysate. Image courtesy of product review submitted by Rasha Elbaz.](http://images.novusbio.com/fullsize/CCR2-Antibody-Western-Blot-NBP1-48337-img0005.jpg)
![Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48337] - An intracellular stain was performed on U2-OS cells with CCR2 NBP1-48337AF647 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/CCR2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48337-img0020.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE tissue section of human liver cancer using 5 ug/ml concentration of CCR2 antibody. Intense cytoplasmic-membranous immune-staining was observed in the cancerous hepatocytes, whereas the staining was relatively weak in the cells adjacent to the fibrotic areas. [Magnification 40X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0011.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE tissue section of human rectal adenocarcinoma using 5 ug/ml concentration of CCR2 antibody. The cancerous cells in the section developed a uniform but specific CCR2 immunopositivity whereas a weak staining was seen in the tumor stroma cells. [Magnification 40X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0012.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE section of human esophageal squamous cell carcinoma (SCC) using 5 ug/ml concentration of CCR2 antibody. Strong cytoplasmic-membranous immune-staining was observed in SCC cells and a relatively weaker staining was seen in the tumor stroma cells. [Magnification 10X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0013.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE tissue section of human normal kidney using 5 ug/ml concentration of CCR2 antibody. Strong cytoplasmic immunostaining was observed in the various renal cells (especially from tubules/ducts epithelium). The cells of Bowman's capsule depicted a very weak cytoplasmic staining for CCR2 protein. [Magnification 10X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0014.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - Analysis of FFPE tissue section of human normal breast using 5 ug/ml concentration of CCR2 antibody. The breast ductal/acinar epithelial cells showed a strong cytoplasmic-membranous CCR2 immune-positivity, whereas the intra-lobular connective tissue depicted weak staining. [Magnification 40X]](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-Paraffin-NBP1-48337-img0015.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48337] - An intracellular stain was performed on THP-1 cells with CCR Antibody NBP1-48337R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48337-img0016.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48337] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48337 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48337-img0018.jpg)
![Flow Cytometry: CCR2 Antibody - BSA Free [NBP1-48337] - An intracellular stain was performed on U2-OS cells with CCR2 NBP1-48337 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR2-Antibody---BSA-Free-Flow-Cytometry-NBP1-48337-img0019.jpg)
![Western Blot: TLR4 Antibody [NB100-56581] - Analysis of TLR4 using NB100-56581 at 2 ug/mL on partial recombinant mouse TLR4 protein.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NB100-56581-img0008.jpg "Western Blot: TLR4 Antibody [NB100-56581] - Analysis of TLR4 using NB100-56581 at 2 ug/mL on partial recombinant mouse TLR4 protein.")
![Immunocytochemistry/Immunofluorescence: TLR4 Antibody [NB100-56581] - TLR4 antibody was tested in PC-12 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56581-img0007.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody [NB100-56581] - Analysis of TLR4 in FFPE mouse spleen tissue using NB100-56581 (A) and mouse isotype control (B) at 5 mg/mL.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-Paraffin-NB100-56581-img0009.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48338-img0004.jpg "Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.")
![Immunocytochemistry/Immunofluorescence: CCR2 Antibody [NBP1-48338] - Analysis of CCR2 in NIH/3T3 cells.](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48338-img0003.jpg)
![Immunohistochemistry: CCR2 Antibody [NBP1-48338] - Expression patterns of CCL2 and CCR2 in unwounded and wounded corneas of WT and MMP12 KO mice. Immunofluorescence of CCL2 chemokine receptor CCR2 in unwounded and chemically wounded (2-days after injury) WT and Mmp12-/- mouse corneas. Control images represent mouse corneas stained with secondary antibody only and without primary antibody. Nuclei were visualized by staining with DAPI (blue). Scale bars: 50 um. CCR2 staining was visualized in epithelial and stromal layers of unwounded and wounded WT and Mmp12-/- corneas. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47831-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CCR2-Antibody-Immunohistochemistry-NBP1-48338-img0007.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48338] - An intracellular stain was performed on THP-1 cells with CCR2 Antibody NBP1-48338F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48338-img0006.jpg)
![Flow Cytometry: CCR2 Antibody [NBP1-48338] - The Alexa Fluor 488 conjugate of this antibody was used: Mouse peripheral blood mononuclear cells were unstained (left) or stained (right) with CCR2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CCR2-Antibody-Flow-Cytometry-NBP1-48338-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CCR2 Antibody - BSA Free [NBP1-48338] - Expression patterns of CCL2 & CCR2 in unwounded & wounded corneas of WT & MMP12 KO mice. (A) Immunofluorescence of CCL2 chemokine & (B) its receptor CCR2 in unwounded & chemically wounded (2-days after injury) WT & Mmp12−/− mouse corneas. Control images represent mouse corneas stained with secondary antibody only & without primary antibody. Nuclei were visualized by staining with DAPI (blue). Scale bars: 50 µm. A magnified image of a wounded WT cornea shows perinuclear expression of CCL2 (orange box). CCL2 staining was visualized in epithelial, stromal, & endothelial layers of wounded WT & Mmp12−/− corneas. CCR2 staining was visualized in epithelial & stromal layers of unwounded & wounded WT & Mmp12−/− corneas. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31399604), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-48338_rabbit-polyclonal-ccr2-antibody-310202415175260.jpg)
![Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 antibody, anti-rat APC conjugated secondary antibody and cell surface flow kit. Green: isotype control. Red: TLR4 antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cell-Surface-NB100-56560-img0010.jpg "Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 antibody, anti-rat APC conjugated secondary antibody and cell surface flow kit. Green: isotype control. Red: TLR4 antibody.")
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 in mouse peritoneal cells. Green: isotype control. Red: anti-TLR4 antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cytometry-NB100-56560-img0009.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram: Cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cytometry-NB100-56560-img0012.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of cell surface TLR4 using this antibody at 0.625 ug/10^6 Raw cells (pretreated with anti-CD16/32). Shaded histogram: isotype control.](http://images.novusbio.com/fullsize/TLR4-Antibody-MTS510-Flow-Cytometry-NB100-56560-img0011.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/nb100-56560_rat-monoclonal-tlr4-antibody-mts510-17720249362619.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in RAW cells using 0.5 ug of this antibody. Black histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/nb100-56560_rat-monoclonal-tlr4-antibody-mts510-17720241003224.jpg)
![Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.](http://images.novusbio.com/fullsize/nb100-56560_rat-monoclonal-tlr4-antibody-mts510-17720241003220.jpg)
![Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Western-Blot-NBP2-27369-img0005.jpg "Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: MyD88 Antibody (4D6) [NBP2-27369] - Analysis of MyD88 antibody in MCF-7 cells using an isotype control (top) and MyD88 Antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Immunocytochemistry-Immunofluorescence-NBP2-27369-img0002.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on Jurkat cells with MyD88 Antibody (4D6) NBP2-27369PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0012.jpg)
![Western Blot: MyD88 Antibody (4D6) [NBP2-27369] - Analysis of MyD88 in A) human ovary, B) human prostate and Jurkat cell lysate in the C) absence and D) presence of immunizing peptide using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Western-Blot-NBP2-27369-img0001.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - Intracellular analysis of MyD88 antibody in Jurkat cells using 0.5 ug. Shaded histogram represents cells without antibody; green represents isotype control; red represents MyD88 antibody. This antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Cytometry-NBP2-27369-img0003.jpg)
![Flow (Intracellular): MyD88 Antibody (4D6) [NBP2-27369] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-27369. Staining of MyD88 antibody in Jurkat cells using 10 ul (0.5 ug) of MyD88 antibody. Green represents isotype control (20108); red represents anti-MyD88 antibody.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Intracellular-NBP2-27369-img0006.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 (4D6) antibody NBP2-27369PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Cytometry-NBP2-27369-img0007.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 [4D6] Antibody NBP2-27369AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-Flow-Cytometry-NBP2-27369-img0008.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on Jurkat cells with MyD88 Antibody (4D6) NBP2-27369 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0009.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on U-937 cells with MyD88 Antibody (4D6) NBP2-27369 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0010.jpg)
![Flow Cytometry: MyD88 Antibody (4D6) - BSA Free [NBP2-27369] - An intracellular stain was performed on MCF7 cells with MyD88 Antibody (4D6) NBP2-27369F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/MyD88-Antibody-4D6-BSA-Free-Flow-Cytometry-NBP2-27369-img0011.jpg)
![Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.](http://images.novusbio.com/fullsize/CCR1-Antibody-Western-Blot-NB100-56334-img0002.jpg "Western Blot: CCR1 Antibody [NB100-56334] - A375 lysate probed with this antibody at 2 ug/ml dilution.")
![Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on Raw264.7 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Intracellular-NB100-56334-img0008.jpg)
![Flow Cytometry: CCR1 Antibody [NB100-56334] - Image 1: Surface staining of mouse primary epidermal cells (B-6 mice, 30wks) with CL 11-PE, CD11c PE-Cy5, and this antibody with CY5 goat anti rabbit secondary. Image 2: CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 ul (blue histogram) and 10 ul (green histogram). Data kindly provided by Corey Parlet, University of Iowa.](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Cytometry-NB100-56334-img0004.jpg)
![Flow (Intracellular): CCR1 Antibody [NB100-56334] - An intracellular stain was performed on U-87 cells with CCR1 Antibody NB100-56334 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CCR1-Antibody-Flow-Intracellular-NB100-56334-img0007.jpg)
![Western Blot: CXCR3 Antibody [NB100-56404] - HEK293T cells. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Western-Blot-NB100-56404-img0004.jpg "Western Blot: CXCR3 Antibody [NB100-56404] - HEK293T cells. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: CXCR3 Antibody [NB100-56404] - Brain microvascular endothelial cells isolated from WT and CXCR3 KO mice were stained with anti-CXCR3 (green) and anti-ZO-1 (blue) antibodies to analyze CXCR3 expression by immunofluorescence. Image from verified customer review.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56404-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404] - IHC analysis of a FFPE human spleen using 1:25 dilution of CXCR3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of HIER using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (40X) was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Immunohistochemistry-Paraffin-NB100-56404-img0005.jpg)
![Western Blot: CXCR3 Antibody [NB100-56404] - Analysis of CXCR3 in human pancreas lysate using CXCR3 antibody at 2 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/CXCR3-Antibody-Western-Blot-NB100-56404-img0003.jpg)
![Western Blot: CCR2 Antibody (3G7) [NBP2-35334] - Western blot analysis of CCR2 [3G7] in Molt4 lysate.](http://images.novusbio.com/fullsize/CCR2-Antibody-3G7-Western-Blot-NBP2-35334-img0007.jpg "Western Blot: CCR2 Antibody (3G7) [NBP2-35334] - Western blot analysis of CCR2 [3G7] in Molt4 lysate.")
![Immunohistochemistry-Paraffin: CCR2 Antibody (3G7) [NBP2-35334] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast using CCR2 antibody (clone 3G7) at 5 ug/ml concentration. The glandular cells in the lobules showed a specific cytoplasmic-membranous immunostaining of CCR2.](http://images.novusbio.com/fullsize/CCR2-Antibody-3G7-Immunohistochemistry-Paraffin-NBP2-35334-img0006.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (3G7) [NBP2-35334] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human HCC (hepatocellular carcinoma / malignant hepatoma) using CCR2 antibody (clone 3G7) at 5 ug/ml concentration. The cancer cells depicted specific punctate to diffused cytoplasmic-membranous immuno-staining of CCR2 protein.](http://images.novusbio.com/fullsize/CCR2-Antibody-3G7-Immunohistochemistry-Paraffin-NBP2-35334-img0003.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (3G7) [NBP2-35334] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using CCR2 antibody (clone 3G7) at 5 ug/ml concentration. The cancer cells depicted specific cytoplasmic-membranous immunostaining, whereas the cells of tumor stroma were largely negative for CCR2 immunopositivity.](http://images.novusbio.com/fullsize/CCR2-Antibody-3G7-Immunohistochemistry-Paraffin-NBP2-35334-img0005.jpg)
![Western Blot: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 using TLR4 antibody at 2 ug/mL on partial recombinant mouse TLR4 protein.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP2-24821-img0001.jpg "Western Blot: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 using TLR4 antibody at 2 ug/mL on partial recombinant mouse TLR4 protein.")
![Immunocytochemistry/Immunofluorescence: TLR4 Antibody [NBP2-24821] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-TLR4 [NBP2-24821] at a 1:50 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24821-img0006.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody [NBP2-24821] - Analysis of TLR4 in FFPE (A) normal and (B) tumor prostate sections using TLR4 antibody at 1:50 dilution. ICC analysis of TLR4 in formalin-fixed (C) Ramos and (D) 293 cells using antibody at 1:10 dilution. Data courtesy of Dr. Regina Gandour-Edwards.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-Paraffin-NBP2-24821-img0002.jpg)
![Flow Cytometry: TLR4 Antibody [NBP2-24821] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-24821. Staining of RAW cells were probed using 2 ug of TLR4 antibody (red) and 2 ug of isotype control antibody (green). Novus's TLR cell surface flow kit (10099K) was used for this test.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP2-24821-img0005.jpg)
![Flow Cytometry: TLR4 Antibody [NBP2-24821] - Cell surface analysis using TLR4 antibody. Mouse macrophage cells were probed using 1 ug of TLR4 antibody (red) and 1 ug of isotype control antibody (green). This antibody and PE-conjugated secondary were used for this test.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP2-24821-img0003.jpg)
![Western Blot: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.](http://images.novusbio.com/fullsize/EBI3-Antibody-15k8D10-Tissue-Culture-Substratum-NBP2-27362-img0001.jpg "Western Blot: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.")
![Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) [NBP2-27362] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) using peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/EBI3-Antibody-15k8D10-Immunohistochemistry-Paraffin-NBP2-27362-img0002.jpg)