| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50419-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24552SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| BSA Free | NBP2-76810 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-93523-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44018 | |||
| BSA Free | NBP2-30699-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43790 | |||
| BSA Free | NBP2-46630-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87760-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88966-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76538-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73788 | |||
![Western Blot: CD97 Antibody [NBP2-24552] - Analysis of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-24552 at 4 ug/ml.](http://images.novusbio.com/fullsize/CD97-Antibody-Western-Blot-NBP2-24552-img0001.jpg "Western Blot: CD97 Antibody [NBP2-24552] - Analysis of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-24552 at 4 ug/ml.")
![Immunohistochemistry-Paraffin: CD97 Antibody [NBP2-24552] - IHC analysis of human spleen using NBP2-24552 at 10 ug/ml.](http://images.novusbio.com/fullsize/CD97-Antibody-Immunohistochemistry-Paraffin-NBP2-24552-img0002.jpg)
![Simple Western: CD97 Antibody [NBP2-24552] - Simple Western lane view shows a specific band for CD97 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CD97-Antibody-Simple-Western-NBP2-24552-img0003.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Western-Blot-NBP2-80713-img0001.jpg "Western Blot: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80713-img0002.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80713-img0003.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) - Azide and BSA Free [NBP2-80713] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit Anti- EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80713-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP1-93523] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-93523-img0004.jpg "Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP1-93523] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Analysis of extracts from RT-4 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-DICER monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Western-Blot-NBP2-30699-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Analysis of extracts from RT-4 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-DICER monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Dicer Antibody (CL0378) [NBP2-30699] - Staining of A-431 cells using the anti-DICER1 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunocytochemistry-Immunofluorescence-NBP2-30699-img0018.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human breast cancer (ductal carcinoma) shows moderate cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0017.jpg)
![Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Lane 1: Marker [kDa]. Lane 2: Human cell line HEK293T](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Western-Blot-NBP2-30699-img0008.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0011.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human testis shows cytoplasmic positivity in the seminiferous tubules cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0012.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human prostate shows cytoplasmic immunoreactivity in the glandular epithelium cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0013.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human cerebral cortex shows cytoplasmic positivity in the glial cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0014.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human colorectal cancer (adenocarcinoma) shows cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0015.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human lung cancer (adenocarcinoma) shows strong cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0016.jpg)
![Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Western-Blot-NBP2-46630-img0008.jpg "Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunocytochemistry/Immunofluorescence: MBP Antibody (CL2819) [NBP2-46630] - Staining of A549 cells using the Anti-MBP monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunocytochemistry-Immunofluorescence-NBP2-46630-img0009.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human tonsil shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0006.jpg)
![Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Western-Blot-NBP2-46630-img0007.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0001.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse cerebral cortex shows strong positivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0002.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human cerebral cortex shows strong immunoreactivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0003.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of mouse striatum shows strong positivity in myelinated fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0004.jpg)
![Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human cerebellum shows strong immunoreactivity in myelinated neural fibers.](http://images.novusbio.com/fullsize/MBP-Antibody-CL2819-Immunohistochemistry-NBP2-46630-img0005.jpg)
![Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87760-img0021.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0020.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody NBP1-87760 (A) shows similar protein distribution across tissues to independent antibody NBP1-87759 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0022.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Analysis using Anti-NFKB2 antibody NBP1-87760 (A) shows similar pattern to independent antibody NBP1-87759 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87760-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87760-img0010.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human testis.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0027.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0018.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0019.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human kidney.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0024.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human lymph node.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0025.jpg)
![Immunohistochemistry-Paraffin: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87760-img0026.jpg)
![Simple Western: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Simple Western lane view shows a specific band for NFKB2 in 0.2 mg/ml of RT4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Simple-Western-NBP1-87760-img0006.jpg)
![Simple Western: NFkB2/NFkB p100 Antibody [NBP1-87760] - NFkB p100/p52 Antibody [NBP1-87760] - Electropherogram image(s) of corresponding Simple Western lane view. NFkB p100/p52 antibody was used at 1:30 dilution on RT4 lysate(s).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Simple-Western-NBP1-87760-img0007.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Knockout-Validated-NBP1-88966-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88966-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88966-img0017.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88966-img0014.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human endometrium shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411124..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411220..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human cerebellum shows negative cytoplasmic positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411310..jpg)
![Western Blot: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Western blot shows lysates of rat lymph node, rat spleen tissue, and rat thymus tissue. PVDF membrane was probed with 0.5 ug/mL of Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD43 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-W3-13-Western-Blot-NB100-64992-img0003.jpg "Western Blot: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Western blot shows lysates of rat lymph node, rat spleen tissue, and rat thymus tissue. PVDF membrane was probed with 0.5 ug/mL of Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD43 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - CD43 was detected in immersion fixed paraffin-embedded sections of rat thymus using Mouse Anti-Rat CD43 Monoclonal Antibody (Catalog # NB100-64992) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was incubated with VisUBlock™ Mouse on Mouse Blocking Reagent (Catalog # VB001) and then subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-W3-13-Immunohistochemistry-Paraffin-NB100-64992-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (W3/13) [NB100-64992] - Staining of rat splenocytes using CD43 FITC.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-W3-13-Flow-Cytometry-NB100-64992-img0001.jpg)
![Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-western-blot-119202416527..gif "<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Loading: 10 μg of lysate Antibodies: RHAMM/CD168 , 1 ug/mL and beta-actin, 1ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: RHAMM/CD168 Antibody [NBP1-76538] - Analysis in rat stomach tissue lysate with antibody at 0.5 ug/mL.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Western-Blot-NBP1-76538-img0004.jpg)
![Immunohistochemistry: RHAMM/CD168 Antibody [NBP1-76538] - Human stomach tissue.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Immunohistochemistry-NBP1-76538-img0003.jpg)
![Flow Cytometry: RHAMM/CD168 Antibody [NBP1-76538] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room temperature. Cells were then washed in 500 uL PBS/BSA/EDTA and viable DAPI cells were analysed using a Galios flow cytometer. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 700 version of this antibody.](http://images.novusbio.com/fullsize/RHAMM-CD168-Antibody-Flow-Cytometry-NBP1-76538-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody - BSA Free [NBP1-76538] - Immunofluorescence of RHAMM/CD168 in human stomach tissue with RHAM antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76538_rabbit-polyclonal-rhamm-cd168-antibody-2711202420243358.jpg)
![Western Blot: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RALBP1 monoclonal antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Western-Blot-NBP2-02293-img0010.jpg "Western Blot: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RALBP1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Immunocytochemistry-Immunofluorescence-NBP2-02293-img0009.jpg)
![Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded prostate tissue using anti-RALBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Immunohistochemistry-Paraffin-NBP2-02293-img0008.jpg)
![Flow Cytometry: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Analysis of Jurkat cells, using anti-RALBP1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Flow-Cytometry-NBP2-02293-img0001.jpg)
![Western Blot: RALBP1 Antibody (OTI11B2) [NBP2-02293] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Western-Blot-NBP2-02293-img0002.jpg)
![Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded breast tissue using anti-RALBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Immunohistochemistry-Paraffin-NBP2-02293-img0003.jpg)
![Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-RALBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Immunohistochemistry-Paraffin-NBP2-02293-img0004.jpg)
![Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-RALBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Immunohistochemistry-Paraffin-NBP2-02293-img0005.jpg)
![Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded endometrium tissue using anti-RALBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Immunohistochemistry-Paraffin-NBP2-02293-img0006.jpg)
![Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded Ovary tissue using anti-RALBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Immunohistochemistry-Paraffin-NBP2-02293-img0007.jpg)
![Immunoprecipitation: RALBP1 Antibody (OTI11B2) [NBP2-02293] - (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RALBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/RALBP1-Antibody-OTI11B2-Immunoprecipitation-NBP2-02293-img0011.jpg)