| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27202SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89104-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56168SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24755SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85814-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21678 | |||
| DyLight 488 | NBP1-77701 | |||
![Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NBP2-27202-img0001.jpg "Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-14-3-3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27202-img0004.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27202-img0002.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27202-img0003.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89104-img0012.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human tonsil shows moderate to strong nuclear positivity in a subset lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human rectum shows moderate to strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0009.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human salivary gland shows moderate to strong nuclear positivity in ductal cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: RUNX2/CBFA1 Antibody [NBP1-89104] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Knockdown-Validated-NBP1-89104-img0014.jpg)
![Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16025-img0013.jpg "Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NBP2-16025] - Human gastric cancer, using CTGF antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-16025-img0007.jpg)
![Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16025-img0009.jpg)
![Western Blot: CTGF/CCN2 Antibody [NBP2-16025] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-Western-Blot-NBP2-16025-img0011.jpg)
![Western Blot: Survivin Antibody [NB100-56168] - Analysis of Normal Prostate Epithelium (NPE) and Prostate Cancer using [NB100-56168]. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE. Bands detected at predicted molecular weight of 16 kDa.](http://images.novusbio.com/fullsize/Survivin-Antibody-Western-Blot-NB100-56168-img0005.jpg "Western Blot: Survivin Antibody [NB100-56168] - Analysis of Normal Prostate Epithelium (NPE) and Prostate Cancer using [NB100-56168]. 100 ug/lane of each human tissue lysate was loaded per lane. Survivin was detected in 7 out of 8 prostate cancer patient specimens, but was not detected in NPE. Bands detected at predicted molecular weight of 16 kDa.")
![Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168] - Analysis of Human Esophageal Cancer at 1:2000 using Survivin Antibody [NB100-56168]. A1 is a high magnification of A. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB100-56168-img0002.jpg)
![Immunohistochemistry-Paraffin: Survivin Antibody [NB100-56168] - Analysis of Human Prostate Cancer array at 1:2000 using Survivin Antibody [NB100-56168]. A1 is a high magnification of A. Differential expression of Survivin is seen in tissue cores from four different prostate cancer patients. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Survivin-Antibody-Immunohistochemistry-Paraffin-NB100-56168-img0004.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0013.jpg "Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on U-2 OS cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Immunocytochemistry-Immunofluorescence-H00000860-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Immunohistochemistry-Paraffin-H00000860-M01-img0010.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in SJCRH30 ( Cat # L027V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0001.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in K-562 ( Cat # L009V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0003.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - RUNX2 monoclonal antibody (M01), clone 1D8. Analysis of RUNX2 expression in Jurkat.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0012.jpg)
![Sandwich ELISA: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Sandwich-ELISA-H00000860-M01-img0011.jpg)
![Western Blot: RUNX2/CBFA1 Antibody [NBP2-24755] - Analysis of human RUNX2 in Saos-2 cell lysate in the 1) absence and 2) presence of immunizing peptide using RUNX2 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Western-Blot-NBP2-24755-img0002.jpg "Western Blot: RUNX2/CBFA1 Antibody [NBP2-24755] - Analysis of human RUNX2 in Saos-2 cell lysate in the 1) absence and 2) presence of immunizing peptide using RUNX2 antibody at 2 ug/mL.")
![Western Blot: Rabbit Polyclonal RUNX2/CBFA1 Antibody [IMGENEX: IMG-5985A] [NBP2-24755] - Bone lysate homogenate from goats. SDS-PAGE with 0.3 mg of total protein from the homogenate per well. RUNX/CBFA1, NBP2-24755 was used at a dilution of 1:1000 and BMP-2, NBP1-32218 was used at a dilution of 1:1000 in TBS Tween and 5% milk powder. The protein was labeled at 70 kDa. Quantification of RUNX2 in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. Evaluation of pre-cellularized graft in the iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to the tibial bone defect, G2: cellularized graft for 6 weeks applied to the tibial bone defect, G3: cellularized graft for 8 weeks applied to the tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 120 days after graft implantation. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-24755_rabbit-polyclonal-runx2-cbfa1-antibody-imgenex-img-5985a-western-blot-2952025142732..jpg)
![Western Blot: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Western Blot analysis of RUNX2 expression in Hela NE ( Cat # L012V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Western-Blot-H00000860-M06-img0001.jpg "Western Blot: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Western Blot analysis of RUNX2 expression in Hela NE ( Cat # L012V1 ).")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Analysis of monoclonal antibody to RUNX2 on U-2 OS cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Immunocytochemistry-Immunofluorescence-H00000860-M06-img0005.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Immunohistochemistry-Paraffin-H00000860-M06-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell . [antibody concentration 10 ug/ml]](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Immunocytochemistry-Immunofluorescence-H00000860-M06-img0003.jpg)
![ELISA: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-ELISA-H00000860-M06-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Analysis in human ovary and skeletal muscle tissues. Corresponding VIM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Analysis in human ovary and skeletal muscle tissues. Corresponding VIM RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Simple-Western-NBP1-85814-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-85814] - Staining of human cell line ASC TERT1 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85814-img0021.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0027.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0024.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0025.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0026.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-85814-img0028.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Simple-Western-NBP1-85814-img0007.jpg)
![Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).](http://images.novusbio.com/fullsize/Vimentin-Antibody-Simple-Western-NBP1-85814-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0011.jpg "Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.")
![Flow Cytometry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the DyLight 488 conjugate of AKT1 phospho Ser473 antibody. Image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Flow-Cytometry-NBP1-69923-img0021.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). A: Lane 1) PDGF stimulated NIH 3T3 cells [10ul]. Lane 2) NIH 3T3 cells [10ul]. Lane 3) Hela whole cell lysate [10ul] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng].Block: 5% BSA overnight at 4C.Primary antibody: Monoclonal anti-AKT antibody used at 1:1000 for overnight at 4C.Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT.Detection: TMB for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - High resolution STED immunofluorescence nanoscopy of AKT11 [p Ser473] antibody (17F6.B11). Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT1 colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min.Antigen retrieval: serum deprivation for 12 h.Primary antibody: AKT1 pS473 antibody at 10 ug/mL and alpha-tubulin (cyan) at 1.4 ug/mL for 1 h at RT.Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight(TM)488 anti-Rabbit IgG were used at 1.0 ug/mL for 1h at RT for indirect detection.Localization: AKT1 pS473 is in the cytoplasm and also organized at the periphery of the cell.Staining: AKT1 pS473 as red signal with bis-benzimide (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0040.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunohistochemistry of AKT11 [p Ser473] antibody (17F6.B11). Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 20 ug/mL for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.Staining: AKT1 pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-NBP1-69923-img0046.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunoblotting analysis. Representative images of the immunoblot analysis for (A) Akt, (B) p53 (C) AMPK, and (D) LC3 at 24 h of incubation with or without 20 mM dG are shown. beta-actin was used as loading control. Densitometry analysis was performed and the Akt-P/Akt ratio was calculated. The results are the mean +/- SEM of three independent experiments. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/7/2115) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0037.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: untreated NIH/3T3 cell lysates. Lane 2: detects phosphorylated AKT1 (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane.Primary antibody: AKT1 pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4 C.Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4 C with FemtoMax(TM) enhanced chemiluminescent reagent.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0042.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane.Primary antibody: AKT1pS473 antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C.Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0043.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated AKT11, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated AKT11 (red band). Load: 10 ug per lane.Primary antibody: rabbit anti-AKT1 (pan) and mouse anti-AKT1 pS473 specific antibodies at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM) 549 conjugated anti-rabbit IgG (green) and DyLight(TM) 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of DyLight 488 conjugate of NBP1-69923. This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923]](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunofluorescence Microscopy of AKT11 [p Ser473] antibody (17F6.B11) using STED nanoscopy to evaluate AKT1 activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins.A massive increase in AKT1-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin.Staining: Panel A shows STED data (AKT1-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT1 is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT1pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0039.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of Biotin conjugate of NBP1-69923. 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear count](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0034.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0036.jpg)
![Western Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 [p Ser473] Antibody (17F6.B11) at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C. Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/nbp1-69923_mouse-monoclonal-akt1-p-ser473-antibody-17f6-b11-255202313136.jpg)
![Western Blot: PRRX1 Antibody [NBP1-06067] - Staining of Mouse Heart ysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PRRX1-Antibody-Western-Blot-NBP1-06067-img0004.jpg "Western Blot: PRRX1 Antibody [NBP1-06067] - Staining of Mouse Heart ysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry: PRRX1 Antibody [NBP1-06067] - PRRX1 and PRRX2 bind to mir875 promoter region. Immunohistochemistry image showing PRRX1 (green) and DAPI staining of nuclei (blue) in E14 tooth germ. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-61693-w) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PRRX1-Antibody-Immunohistochemistry-NBP1-06067-img0002.jpg)
![Western Blot: PRRX1 Antibody [NBP1-06067] - Staining of LNCaP cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PRRX1-Antibody-Western-Blot-NBP1-06067-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: PRRX1 Antibody [NBP1-06067] - PRRX1 & PRRX2 bind to mir875 promoter region. (a) Diagram of mir875 promoter region. The CAGE peak accurately represents TSS. (b) Immunohistochemistry image showing PRRX1 (green) & DAPI staining of nuclei (blue) in E14 tooth germ. (c) qRT-PCR analysis of Prrx1 & Prrx2 expressions in teeth obtained from E13 to P7 after normalization to Gapdh expression. (d) Schematic diagram showing luciferase reporter vector of mir875 promoter. PRRX1/2 binding was shown to occur in the mir875 promoter region. TAATTA is a potential binding site for PRRX1/2. (e) Mir875 promoter reporter activities were evaluated in mDP cells transfected with a Prrx1/2 expression vector after 12, 24, & 48 hours of culture. (f) qRT-PCR analysis of miR875-5p expression in mDP cells cultured with or without Prrx1/2 transfection. (g) Sequences of mir875 promoter & mutant promoter. TAATTA was replaced by the TCCCCA sequence. (h) Mir875 promoter reporter activities were evaluated in mDP cells after transfection with a Prrx1 or Prrx2 expression vector. *P < 0.05. Error bars represent the mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32188878), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06067_goat-polyclonal-prrx1-antibody-310202416175246.jpg)
![Western Blot: EGFR Antibody [NBP1-84814] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NBP1-84814-img0009.jpg "Western Blot: EGFR Antibody [NBP1-84814] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP1-84814] - Staining of human cell line A-431 shows positivity in plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84814-img0006.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0017.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0015.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0005.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Biopsies of deparaffinized Human melanoma tissue. Cells were probed without (control) or with or an antibody recognizing Vimentin](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0003.jpg)
![Western Blot: Vimentin Antibody (J144) [NB100-74564] - Analysis of rat brain cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Western-Blot-NB100-74564-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0006.jpg)