| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39007-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44470 | |||
| BSA Free | NBP2-36769-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91938-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-20694 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25150SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37938-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86031-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48942-25ul | |||
![Immunohistochemistry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32399) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-29820241492629.jpg "Immunohistochemistry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32399) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling GOLPH4 with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32399, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-298202414161893.jpg)
![Immunocytochemistry/ Immunofluorescence: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Immunocytochemistry analysis of HeLa cells labeling GOLPH4 with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/1,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-29202410334057.jpg)
![Flow Cytometry: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Flow cytometric analysis of HeLa cells labeling GOLPH4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32399, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-29202416372195.jpg)
![Western Blot: GOLPH4 Antibody (PSH02-98) [NBP3-32399] - Western blot analysis of GOLPH4 on different lysates with Rabbit anti-GOLPH4 antibody (NBP3-32399) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: U-2 OS cell lysate Lane 4: LO2 cell lysate Lane 5: HUVEC cell lysate Lane 6: SiHa cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 82 kDa Observed band size: 130/82 kDa Exposure time: Lane 1-6 (left): 1 minute 2 seconds; Lane 1-6 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32399_rabbit-golph4-mab-psh02-98-3920248485014.jpg)
![Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP2-39007] - Staining in human epididymis and skeletal muscle tissues using NBP2-39007 antibody. Corresponding GOLGA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Immunohistochemistry-Paraffin-NBP2-39007-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP2-39007] - Staining in human epididymis and skeletal muscle tissues using NBP2-39007 antibody. Corresponding GOLGA5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP2-39007] - Staining of human cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Immunohistochemistry-Paraffin-NBP2-39007-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP2-39007] - Staining of human cerebellum, epididymis, stomach and testis using Anti-GOLGA5 antibody NBP2-39007 (A) shows similar protein distribution across tissues to independent antibody NBP1-83352 (B).](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Immunohistochemistry-Paraffin-NBP2-39007-img0011.jpg)
![Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP2-39007] - Staining of human epididymis shows strong positivity in Golgi apparatus in glandular cells.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Immunohistochemistry-Paraffin-NBP2-39007-img0012.jpg)
![Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP2-39007] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Immunohistochemistry-Paraffin-NBP2-39007-img0013.jpg)
![Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP2-39007] - Staining of human stomach shows moderate to strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Immunohistochemistry-Paraffin-NBP2-39007-img0014.jpg)
![Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP2-39007] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Immunohistochemistry-Paraffin-NBP2-39007-img0015.jpg)
![Western Blot: GRASP55 Antibody (CL2522) [NBP2-36769] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2522-Western-Blot-NBP2-36769-img0006.jpg "Western Blot: GRASP55 Antibody (CL2522) [NBP2-36769] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG")
![Immunohistochemistry: GRASP55 Antibody (CL2522) [NBP2-36769] - Staining of human skeletal muscle shows absence of immunoreactivity.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2522-Immunohistochemistry-NBP2-36769-img0005.jpg)
![Immunohistochemistry: GRASP55 Antibody (CL2522) [NBP2-36769] - Staining of human small intestine shows cytoplasmic and membranous immunoreactivity in glandular epithelium and lamina propria cells.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2522-Immunohistochemistry-NBP2-36769-img0001.jpg)
![Immunohistochemistry: GRASP55 Antibody (CL2522) [NBP2-36769] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2522-Immunohistochemistry-NBP2-36769-img0002.jpg)
![Immunohistochemistry: GRASP55 Antibody (CL2522) [NBP2-36769] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2522-Immunohistochemistry-NBP2-36769-img0003.jpg)
![Immunohistochemistry: GRASP55 Antibody (CL2522) [NBP2-36769] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/GRASP55-Antibody-CL2522-Immunohistochemistry-NBP2-36769-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP1-91938] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91938-img0005.jpg "Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP1-91938] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.")
![Western Blot: GOLGA5 Antibody [NBP1-31862] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Western-Blot-NBP1-31862-img0005.jpg "Western Blot: GOLGA5 Antibody [NBP1-31862] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GOLGA5 Antibody [NBP1-31862] - Analysis of methanol-fixed HeLa, using GOLGA5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31862-img0002.jpg)
![Immunohistochemistry-Paraffin: GOLGA5 Antibody [NBP1-31862] - Paraffin-embedded smooth muscle, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Immunohistochemistry-Paraffin-NBP1-31862-img0003.jpg)
![Western Blot: GOLGA5 Antibody [NBP1-31862] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/GOLGA5-Antibody-Western-Blot-NBP1-31862-img0004.jpg)
![Western Blot: VPS45 Antibody [NB100-41372] - Staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VPS45-Antibody-Western-Blot-NB100-41372-img0002.jpg "Western Blot: VPS45 Antibody [NB100-41372] - Staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-41372-img0006.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-41372-img0004.jpg)
![Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Placenta.Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS45-Antibody-Immunohistochemistry-Paraffin-NB100-41372-img0005.jpg)
![Western Blot: ABCA7 Antibody [NBP2-82069] - Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Western-Blot-NBP2-82069-img0001.jpg "Western Blot: ABCA7 Antibody [NBP2-82069] - Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: ABCA7 Antibody [NBP2-82069] - Immunocytochemistry of ABCA7 in 293 cells with ABCA7 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82069-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA7 Antibody - BSA Free [NBP2-82069] - Immunofluorescence of ABCA7 in human spleen tissue with ABCA7 antibody at 20 ug/ml.Red: ABCA7 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82069_rabbit-polyclonal-abca7-antibody-271120241958468.gif)
![Immunohistochemistry: ABCA7 Antibody - BSA Free [NBP2-82069] - Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82069_rabbit-polyclonal-abca7-antibody-2711202419592055.gif)
![Immunocytochemistry/ Immunofluorescence: ABCA7 Antibody - BSA Free [NBP2-82069] - Immunofluorescence of ABCA7 in 293 cells with ABCA7 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82069_rabbit-polyclonal-abca7-antibody-271120242022969.jpg)
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha 1 Mannosidase 1A Antibody [NBP2-37938] - Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/alpha-1-Mannosidase-1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37938-img0004.jpg "Immunocytochemistry/Immunofluorescence: alpha 1 Mannosidase 1A Antibody [NBP2-37938] - Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.")
![Immunohistochemistry-Paraffin: alpha 1 Mannosidase 1A Antibody [NBP2-37938] - Staining of human liver shows moderate positivity in bile canaliculi.](http://images.novusbio.com/fullsize/alpha-1-Mannosidase-1A-Antibody-Immunohistochemistry-Paraffin-NBP2-37938-img0007.jpg)
![Immunohistochemistry: alpha 1 Mannosidase 1A Antibody [NBP2-37938] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/alpha-1-Mannosidase-1A-Antibody-Immunohistochemistry-NBP2-37938-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha 1 Mannosidase 1A Antibody [NBP2-37938] - Staining of human duodenum shows strong positivity in Golgi apparatus in glandular cells.](http://images.novusbio.com/fullsize/alpha-1-Mannosidase-1A-Antibody-Immunohistochemistry-Paraffin-NBP2-37938-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha 1 Mannosidase 1A Antibody [NBP2-37938] - Staining of human kidney shows moderate to strong positivity in Golgi apparatus in cells in tubules.](http://images.novusbio.com/fullsize/alpha-1-Mannosidase-1A-Antibody-Immunohistochemistry-Paraffin-NBP2-37938-img0006.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 2/PCSK2 Antibody [NBP2-48942] - Staining in human adrenal gland and pancreas tissues using anti-PCSK2 antibody. Corresponding PCSK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Proprotein-Convertase-2-PCSK2-Antibody-Immunohistochemistry-Paraffin-NBP2-48942-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Proprotein Convertase 2/PCSK2 Antibody [NBP2-48942] - Staining in human adrenal gland and pancreas tissues using anti-PCSK2 antibody. Corresponding PCSK2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Proprotein Convertase 2/PCSK2 Antibody [NBP2-48942] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/Proprotein-Convertase-2-PCSK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48942-img0002.jpg)
![Immunohistochemistry: Proprotein Convertase 2/PCSK2 Antibody [NBP2-48942] - Staining of human small intestine shows strong cytoplasmic positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Proprotein-Convertase-2-PCSK2-Antibody-Immunohistochemistry-NBP2-48942-img0001.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 2/PCSK2 Antibody [NBP2-48942] - Staining of human adrenal gland shows strong cytoplasmic positivity in adrenal medulla.](http://images.novusbio.com/fullsize/Proprotein-Convertase-2-PCSK2-Antibody-Immunohistochemistry-Paraffin-NBP2-48942-img0004.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 2/PCSK2 Antibody [NBP2-48942] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Proprotein-Convertase-2-PCSK2-Antibody-Immunohistochemistry-Paraffin-NBP2-48942-img0005.jpg)