| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08380 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12876-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-77072 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55509-25ul | |||
![Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Western Blot of Bovine Serum Albumin antibody (5F9). Lane 1: Bovine Serum Albumin. Load: 50 ng per lane.Primary antibody: Bovine Serum Albumin antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT.Block: 0.75% Casein for 30 min at RT.Predicted/Observed size: 60 kDa, 60 kDa for Bovine Serum Albumin.](http://images.novusbio.com/fullsize/Bovine-Serum-Albumin-Antibody-5F9-Western-Blot-NB600-868-img0007.jpg "Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Western Blot of Bovine Serum Albumin antibody (5F9). Lane 1: Bovine Serum Albumin. Load: 50 ng per lane.Primary antibody: Bovine Serum Albumin antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT.Block: 0.75% Casein for 30 min at RT.Predicted/Observed size: 60 kDa, 60 kDa for Bovine Serum Albumin.")
![Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Bovine Serum Albumin Antibody (5F9) is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2).](http://images.novusbio.com/fullsize/Bovine-Serum-Albumin-Antibody-5F9-Western-Blot-NB600-868-img0002.jpg)
![ELISA: Bovine Serum Albumin Antibody (5F9) [NB600-868] - ELISA Results of Bovine Serum Albumin antibody (5F9). Each well was coated in duplicate with 10 ug of Bovine Serum Albumin. The working dilution is 1:85,300. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Rabbit Anti-Mouse HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Bovine-Serum-Albumin-Antibody-5F9-ELISA-NB600-868-img0008.jpg)
![Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - ST6GAL1 antibody [HL2318] detects ST6GAL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ST6GAL1 stained by ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25695_rabbit-st6-gal-sialyltransferase-1-st6gal1-cd75-mab-hl2318-azide-bsa-free-2120249355572.jpg "Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - ST6GAL1 antibody [HL2318] detects ST6GAL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ST6GAL1 stained by ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - ST6GAL1 antibody [HL2318] detects secreted ST6GAL1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. ST6GAL1 stained by ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25695_rabbit-st6-gal-sialyltransferase-1-st6gal1-cd75-mab-hl2318-azide-bsa-free-2120249371598.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25695_rabbit-st6-gal-sialyltransferase-1-st6gal1-cd75-mab-hl2318-azide-bsa-free-2120249432831.jpg)
![Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (HL2318) - Azide and BSA Free [NBP3-25695] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ST6GAL1 antibody [HL2318] (NBP3-25695) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25695_rabbit-st6-gal-sialyltransferase-1-st6gal1-cd75-mab-hl2318-azide-bsa-free-2120249351324.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-HSP90B1-3168R-Immunohistochemistry-Paraffin-NBP3-07740-img0003.jpg "Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).")
![Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Flow Cytometric Analysis of PFA-fixed HePG2 cells using gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-HSP90B1-3168R-Flow-Cytometry-NBP3-07740-img0001.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (HSP90B1/3168R) [NBP3-07740] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with gp96/HSP90B1/GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-HSP90B1-3168R-Immunohistochemistry-Paraffin-NBP3-07740-img0002.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Western Blot Analysis of HeLa cell lysate using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Western-Blot-NBP3-07764-img0004.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Western Blot Analysis of HeLa cell lysate using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).")
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded Rat Heart Muscle stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Immunohistochemistry-Paraffin-NBP3-07764-img0003.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded human Breast stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Immunohistochemistry-Paraffin-NBP3-07764-img0002.jpg)
![Protein Array: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Analysis of Protein Array containing more than 19,000 full-length human proteins using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CRYAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Protein-Array-NBP3-07764-img0001.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Golgin 97 antibody [HL3788] (NBP3-48847) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48847_rabbit-golgin-97-mab-hl3788-western-blot-1411202518391021.jpg "Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Golgin 97 antibody [HL3788] (NBP3-48847) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Golgin 97 antibody [HL3788] detects Golgin 97 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Golgin 97 stained by Golgin 97 antibody [HL3788] (NBP3-48847) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48847_rabbit-golgin-97-mab-hl3788-immunocytochemistry-immunofluorescence-141120251838176.jpg)
![Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Golgin 97 antibody [HL3788] (NBP3-48847) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48847_rabbit-golgin-97-mab-hl3788-western-blot-1411202518405411.jpg)
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Golgin 97 antibody [HL3788] (NBP3-48847) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48847_rabbit-golgin-97-mab-hl3788-western-blot-1411202518352211.jpg)
![ELISA: Human Albumin ELISA Kit (Colorimetric) [NBP2-60461]](http://images.novusbio.com/fullsize/Human-Albumin-ELISA-Kit-Colorimetric-ELISA-NBP2-60461-img0001.jpg "ELISA: Human Albumin ELISA Kit (Colorimetric) [NBP2-60461]")
![ELISA: Human Albumin ELISA Kit (Colorimetric) [NBP2-60461] - Albumin calibration curve. Image from verified customer review.](http://images.novusbio.com/fullsize/Human-Albumin-ELISA-Kit-Colorimetric-ELISA-NBP2-60461-img0002.jpg)
![Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/GRASP65-Antibody-3F2I1-Western-Blot-NBP3-16113-img0002.jpg "Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: GRASP65 Antibody (3F2I1) [NBP3-16113] - Western blot analysis of extracts of various cell lines, using GRASP65 Rabbit mAb (NBP3-16113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/GRASP65-Antibody-3F2I1-Western-Blot-NBP3-16113-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] - Confocal imaging of U-2 OS cells using GRASP65 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16113_rabbit-grasp65-mab-3f2i1-7220251245347.jpg)
![Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (3F2I1) [NBP3-16113] - Confocal imaging of Hep G2 cells using GRASP65 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16113_rabbit-grasp65-mab-3f2i1-72202512435346.jpg)
![Western Blot: Golgin 97 Antibody [NBP2-81753] - Western blot analysis of Golgin 97 in human stomach tissue lysate with Golgin 97 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking petpide.](http://images.novusbio.com/fullsize/Golgin-97-Antibody-Western-Blot-NBP2-81753-img0001.jpg "Western Blot: Golgin 97 Antibody [NBP2-81753] - Western blot analysis of Golgin 97 in human stomach tissue lysate with Golgin 97 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking petpide.")
![Immunocytochemistry/ Immunofluorescence: Golgin 97 Antibody - BSA Free [NBP2-81753] - Immunofluorescence of Golgin 97 in rat stomach tissue with Golgin 97 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81753_rabbit-polyclonal-golgin-97-antibody-2711202419381714.jpg)
![Immunohistochemistry: Golgin 97 Antibody - BSA Free [NBP2-81753] - Immunohistochemistry of Golgin 97 in rat stomach tissue with Golgin 97 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81753_rabbit-polyclonal-golgin-97-antibody-271120241935384.jpg)
![Western Blot: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (NBP2-12876). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12876) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP40-DNAJB1-Antibody-3B9-E6-Western-Blot-NBP2-12876-img0007.jpg "Western Blot: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (NBP2-12876). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12876) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry-Paraffin: HSP40/DNAJB1 Antibody (3B9.E6) [NBP2-12876] - Hsp40 HDJ1 visulaized using, tested on a human keratinocyte line HaCat. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/HSP40-DNAJB1-Antibody-3B9-E6-Immunohistochemistry-Paraffin-NBP2-12876-img0004.jpg)
![Western Blot: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Western blot analysis of HSP90 beta on different lysates with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Mouse heart tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-278202415212484.jpg "Western Blot: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Western blot analysis of HSP90 beta on different lysates with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C6 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Mouse heart tissue lysate (40 ug/Lane) Lane 11: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32446) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-298202414333651.jpg)
![Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (2-1-G3-R) [NBP3-32446] - Immunocytochemistry analysis of HeLa cells labeling HSP90 beta with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSP90 beta antibody (NBP3-32446) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32446_mouse-hsp90-beta-mab-2-1-g3-r-2920241048485.jpg)
![Western Blot: Notch-2 Antibody [NBP2-27347] - Analysis of NOTCH2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27347 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NBP2-27347-img0002.jpg "Western Blot: Notch-2 Antibody [NBP2-27347] - Analysis of NOTCH2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27347 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Knockdown-Validated-NBP2-55509-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP90 beta Antibody [NBP2-55509] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP2-55509) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: HSP90 beta Antibody [NBP2-55509] - Analysis using Anti-HSP90AB1 antibody NBP2-55509 (A) shows similar pattern to independent antibody NBP2-68978 (B).](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Western-Blot-NBP2-55509-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP2-55509] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HSP90AB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55509-img0001.jpg)