| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59627SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NB120-6641-1000ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Non-Recombinant Monoclonal | NB100-64768SS | |||
| Azide and BSA Free | NBP2-80768 | |||
| BSA Free | NBP3-07011SS | |||
| BSA Free | NBP3-07011 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80547 | |||
| BSA Free | NBP3-09171SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26047 | |||
![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - used for Western Blot. Lane 1: Untransfected HEK (control) Lane 2: Transfected HEK (FLAG-tagged protein, clone 1) Lane 3: Transfected HEK (FLAG-tagged protein, clone 2). Image from verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-345-img0008.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - used for Western Blot. Lane 1: Untransfected HEK (control) Lane 2: Transfected HEK (FLAG-tagged protein, clone 1) Lane 3: Transfected HEK (FLAG-tagged protein, clone 2). Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-345] - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-345] used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-345-img0007.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-345] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-345-img0002.jpg)
![Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis using FITC conjugate of NB600-305. 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase diluted to 1:10,000. Reaction occurred for 2 hours at room temperature.](http://images.novusbio.com/fullsize/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0017.jpg "Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis using FITC conjugate of NB600-305. 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase diluted to 1:10,000. Reaction occurred for 2 hours at room temperature.")
![Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).](http://images.novusbio.com/fullsize/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0014.jpg)
![Western Blot: beta Galactosidase Antibody [NB600-305] - Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. Blots were blocked overnight at 4 C with Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with anti-b-Galactosidase diluted to 1:10,000. Reaction occurred overnight at 4C. Dylight649 conjugated Gt-a-anti-Rabbit IgG was used for detection.](http://images.novusbio.com/fullsize/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0015.jpg)
![Western Blot: beta Galactosidase Antibody [NB600-305] - Shows a band at 117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase diluted to 1:10,000. Reaction occurred for 2 hours at room temperature.](http://images.novusbio.com/fullsize/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0016.jpg)
![Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: Beta-Galactosidase Reduced [0.1ug]. Lane 2: Opal Prestained Molecular Weight Marker](http://images.novusbio.com/fullsize/nb600-305_rabbit-polyclonal-beta-galactosidase-antibody-24520231056468.jpg)
![Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: partially purified preparation b-Galactosidase [1.0ug]. Lane 2: Molecular Weight Marker. Primary Antibody: Anti-Beta-Galactosidase at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG IRDye(R)800](http://images.novusbio.com/fullsize/nb600-305_rabbit-polyclonal-beta-galactosidase-antibody-2552023131130.jpg)
![Western Blot: Strep II Tag Antibody (517) [NBP2-43735] - Sample from human breast cell lysate (MDA-MB-231 cells). Absence of a band in the lane containing lysate from cells with empty vector confirm antibody is specific for Strep II Tag. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Strep-II-Tag-Antibody-517-Western-Blot-NBP2-43735-img0003.jpg "Western Blot: Strep II Tag Antibody (517) [NBP2-43735] - Sample from human breast cell lysate (MDA-MB-231 cells). Absence of a band in the lane containing lysate from cells with empty vector confirm antibody is specific for Strep II Tag. Image submitted by a verified customer review.")
![Western Blot: Strep II Tag Antibody (517) [NBP2-43735] - PKN3 regulates cell migration in 3D environment of SrcF-transformed MEFs and tumor growth in vivo through interaction with p130Cas. Association of PKN3 with Src and Actin in p130Cas-dependent manner is shown by co-immunoprecipitations. SrcF-transformed cells with or without p130Cas were transfected by Strep-PKN3, and PKN3 was precipitated by Strep-Tactin(R) Superflow(R) resin followed by western blotting and detection by anti-StrepII antibody. Co-immunoprecipitated p130Cas, Src, and Actin were detected using appropriate antibodies (anti-p130Cas, anti-Src, anti-Actin). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/1878-0261.12401) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Strep-II-Tag-Antibody-517-Western-Blot-NBP2-43735-img0004.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT517] (NBP2-43735) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43735_mouse-monoclonal-strep-ii-tag-antibody-517-western-blot-2611202518395710.jpg)
![Strep epitope Tag antibody [GT517] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT517] (NBP2-43735) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43735_mouse-monoclonal-strep-ii-tag-antibody-517-immunocytochemistry-immunofluorescence-261120251841399.jpg)
![Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Simple-Western-NB100-64768-img0002.jpg "Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the Biotin conjugate of NB100-64768. Detection of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with Streptavidin : HRP.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0008.jpg)
![Flow Cytometry: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the FITC conjugate of NB100-64768. Staining of Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Flow-Cytometry-NB100-64768-img0007.jpg)
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Drosophila, 20 ug whole cell lysate](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0012.jpg)
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the HRP conjugate of NB100-64768. Detection of His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0013.jpg)
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis of 1) 6x-His tagged FLCN protein and 2) 6x-His tagged homer protein.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - Intracellular analysis of mouse TLR 6 polyclonal antibody (Red peak), rabbit isotype control (Green peak), RAW cells (Purple peak).Two micrograms of antibodies were used. Goat anti-rabbit FITC was used as secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24893-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - Intracellular analysis of mouse TLR 6 polyclonal antibody (Red peak), rabbit isotype control (Green peak), RAW cells (Purple peak).Two micrograms of antibodies were used. Goat anti-rabbit FITC was used as secondary antibody.")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24893-img0002.jpg)
![Western Blot: AU1 Epitope Tag Antibody [NB600-453] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/mL (1:25,000).](http://images.novusbio.com/fullsize/AU1-Epitope-Tag-Antibody-Western-Blot-NB600-453-img0001.jpg "Western Blot: AU1 Epitope Tag Antibody [NB600-453] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/mL (1:25,000).")
![Western Blot: AU1 Epitope Tag Antibody [NB600-453] - analysis of overexpressed AU1-tagged recombinant proteins in S2 cell extract. Expected lanes are marked with asterisk (*). WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AU1-Epitope-Tag-Antibody-Western-Blot-NB600-453-img0002.jpg)
![Western Blot: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP2-36463-img0028.jpg "Western Blot: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Flow (Intracellular): Rabbit IgG Isotype Control [NBP2-36463] - Intracellular staining for GLI1, GLI2 and GLI3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using a secondary anti-rabbit IgG PE conjugated antibody. Image courtesy of Sherine Elsawa, Northern Illinois University.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Flow-Intracellular-NBP2-36463-img0007.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP2-36463-img0018.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - SDS-Page of Rabbit IgG. Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG. Other Band(s): None.](http://images.novusbio.com/fullsize/Rabbit IgG Isotype Control-SDS-Page-NBP2-36463-img0030.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP2-36463-img0029.jpg)
![Western Blot: c-Myc Antibody [NB600-334] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-c-myc antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-334-img0005.jpg "Western Blot: c-Myc Antibody [NB600-334] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-c-myc antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Analysis using the HRP conjugate of NB600-354. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).](http://images.novusbio.com/fullsize/Glu-Glu-Epitope-Tag-Antibody-Western-Blot-NB600-354-img0003.jpg "Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Analysis using the HRP conjugate of NB600-354. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).")
![Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/Glu-Glu-Epitope-Tag-Antibody-Western-Blot-NB600-354-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Glu-Glu Epitope Tag Antibody [NB600-354] - Triton X-100(1%0 Whole cell lysate(20mcg) from Cos7 cells transiently transfected with control vector (v) or an expression vector driving expression of Glu-Glu tagged RalBP1 (a to d).](http://images.novusbio.com/fullsize/Glu-Glu-Epitope-Tag-Antibody-Western-Blot-NB600-354-img0001.jpg)
![Western Blot: His Tag Antibody (RM146) [NBP2-61482] - Western blot of 293T cells (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y, using NBP2-61482.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RM146-Western-Blot-NBP2-61482-img0008.jpg "Western Blot: His Tag Antibody (RM146) [NBP2-61482] - Western blot of 293T cells (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y, using NBP2-61482.")
![Immunohistochemistry: His Tag Antibody (RM146) [NBP2-61482] - Immunohistochemistry staining of naive HepG2 cells (Negative control) using anti-His-Tag antibody, NBP2-61482.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RM146-Immunohistochemistry-NBP2-61482-img0004.jpg)
![Immunohistochemistry-Paraffin: His Tag Antibody (RM146) [NBP2-61482] - Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using NBP2-61482.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RM146-Immunohistochemistry-Paraffin-NBP2-61482-img0001.jpg)
![Simple Western: His Tag Antibody (RM146) [NBP2-61482] - Human purified protein. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-RM146-Simple-Western-NBP2-61482-img0007.jpg)