beta Galactosidase Antibody


Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis using FITC conjugate of NB600-305. 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations more
Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved more
Western Blot: beta Galactosidase Antibody [NB600-305] - Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the more
Western Blot: beta Galactosidase Antibody [NB600-305] - Shows a band at 117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 more
Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: Beta-Galactosidase Reduced [0.1ug]. Lane 2: Opal Prestained Molecular Weight Marker
Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: partially purified preparation b-Galactosidase [1.0ug]. Lane 2: Molecular Weight Marker. Primary Antibody: Anti-Beta-Galactosidase at 1:1000 overnight at more

Product Details

Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

beta Galactosidase Antibody Summary

Beta-Galactosidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.coli]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Full length native beta Galactosidase isolated from E.coli
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:5000
  • Immunohistochemistry-Frozen 1:1500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation
  • Western Blot 1:5000-1:10000
Application Notes
This product has been tested by ELISA and western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 um) to produce free-floating>
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23838935).

Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25799059)
Reviewed Applications
Read 1 Review rated 5
NB600-305 in the following applications:

Read Publications using
NB600-305 in the following applications:

Reactivity Notes

Cross reactivity against beta Galactosidase from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for beta Galactosidase Antibody

  • EC; b-gal


Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Galactosidase Antibody (NB600-305)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB600-305 Applications Species
Dahlhoff M, Camera E, Schafer M et al. Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis, and ocular integrity in mice. Development. 2016-03-17 [PMID: 26989175] (IHC-Fr, Mouse) IHC-Fr Mouse
Zhao H, Feng J, Ho TV et al. The suture provides a niche for mesenchymal stem cells of craniofacial bones. Nat. Cell Biol. 2015-04-01 [PMID: 25799059] (IHC-Fr, Mouse) IHC-Fr Mouse
Spaeth EL, Labaff AM, Toole BP et al. Mesenchymal CD44 Expression Contributes to the Acquisition of an Activated Fibroblast Phenotype via TWIST Activation in the Tumor Microenvironment. Cancer Res. 2013-09-01 [PMID: 23838935] (IF/IHC, ICC/IF, Mouse) IF/IHC, ICC/IF Mouse
Li B, Pozzi A, Young PP. TNF{alpha} Accelerates Monocyte to Endothelial Transdifferentiation in Tumors by the Induction of Integrin {alpha}5 Expression and Adhesion to Fibronectin. Mol Cancer Res;9(6):702-11. 2011-06-01 [PMID: 21536688]
Li B, Sharpe E E, Maupin A B,et al. FJ EXPRESS: VEGF PIGF promote adult vasculogenesis by enhancing EPC recruitment vessel formation at the site of tumor neovascularization. FASEB J, 20: 1495-1497. 2006-01-01 [PMID: 16754748]
Sharpe, E E, III, Teleron, A A, Li, B, Price, J, Ss, M S, Alford, K, Young, P P. The Origin in Vivo Significance of Murine Human Culture-Exped Endothelial Progenitor Cells, . 168, 1710-1721. 2006-01-01 [PMID: 16651636]
Goldenberg-Cohen N, Guo Y, Margolis F et al. Oligodendrocyte dysfunction after induction of experimental anterior optic nerve ischemia. Invest Ophthalmol Vis Sci 2005-08-01 [PMID: 16043843] (Mouse) Mouse
Condon, J et al. Surfactant protein secreted by the maturing mouse fetal lung acts as a hormone that signals the initiation of parturition. PNAS 101:4978-4983. 2004-01-01 [PMID: 15044702] (WB, IF/IHC, Mouse) WB, IF/IHC Mouse
Farmen, SL et al. Gene transfer of CFTR to airway epithelia: low levels of expression are sufficient to correct Cl-transport overexpression can generate basolateral CFTR. Am. J. Physiol. Lung Cell Mol. Physiol. 289:L1123-L1130. 2005-01-01 [PMID: 12798297]
Tulina N amp. Matunis E Control of stem cell self-renewal in Drosophila spermatogenesis by JAK-STAT signaling. Science 294:2546-9. 2001-01-01 [PMID: 11752575]
Show All 11 Publications.

Review for beta Galactosidase Antibody (NB600-305) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-305:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Western Blot beta Galactosidase NB600-305
reviewed by:
Verified Customer
WB Human 05/05/2019


ApplicationWestern Blot
Sample TestedSH-SY5Y whole cell lysate


CommentsAntibody concentration: 1:2,000
Incubation time/temp: overnight at 4C (shaking)

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Galactosidase Antibody (NB600-305). (Showing 1 - 1 of 1 FAQs).

  1. I have a potential customer who is looking for an antibody to detect Beta Galactosidase in paraffin-embedded transgenic mouse samples. Would antibody NB600-305 work for their experiments? I looked at the reference but I could not find that they mentioned if the tissue samples used were frozen or fixed and embedded in paraffin. Can you help?
    • A couple of publications have mentioned about detail of IHC and you may use following links to have access to the same. For IHC-P, Li B, et al. VEGF and PlGF promote adult vasculogenesis by enhancing EPC recruitment and vessel formation at the site of tumor neovascularization.FASEB J. 2006 Jul;20(9):1495-7. Epub 2006 Jun 5. [PMID: 16754748]. For IHC-Fr, Li B, et al. TNFalpha accelerates monocyte to endothelial transdifferentiation in tumors by the induction of integrin alpha5 expression and adhesion to fibronectin. Mol Cancer Res. 2011 Jun;9(6):702-11. Epub 2011 May 2. [PMID:21536688].

Secondary Antibodies


Isotype Controls

Additional beta Galactosidase Products

Research Areas for beta Galactosidase Antibody (NB600-305)

Find related products by research area.

Blogs on beta Galactosidase

There are no specific blogs for beta Galactosidase, but you can read our latest blog posts.
mFlour Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Recent Reviews


Verified Customer
Application: WB
Species: Human