| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22111SS | |||
| Azide and BSA Free | NBP2-80750 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-336SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-307SS | |||
| Azide and BSA Free | NBP2-80837 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1677SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-108SS | |||
| Azide and BSA Free | NBP2-80674 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-62264-0.025mg | |||
![Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Immunohistochemistry-Frozen-NB810-56910-img0012.jpg "Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.")
![Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Immunohistochemistry-Frozen-NB810-56910-img0011.jpg)
![Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Expression of GFP in HEK293T cells. This image was submitted via customer Review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Western-Blot-NBP2-22111-img0005.jpg "Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Expression of GFP in HEK293T cells. This image was submitted via customer Review. Image using the HRP form of this antibody.")
![Immunocytochemistry/Immunofluorescence: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Immunocytochemistry-Immunofluorescence-NBP2-22111-img0004.jpg)
![Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Western-Blot-NBP2-22111-img0001.jpg)
![Flow Cytometry: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb .](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Flow-Cytometry-NBP2-22111-img0003.jpg)
![Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).](http://images.novusbio.com/fullsize/GFP-Antibody-4B10B2-Western-Blot-NBP2-22111-img0002.jpg)
![Immunohistochemistry: HA Tag Antibody [NB600-362] - SUMO1 conjugates are not localized to amyloid plaques. (a) Sagittal brain sections of 24-week-old KI/AD and WT/AD animals were stained using anti-HA antibodies (red), 6E10 antibodies (green) that label amyloid beta 1-42 among other amyloid beta variants (epitope lies within amino acids 3-8 of amyloid beta) and anti-MAP2 antibodies (blue). Sections of the hippocampal subiculum are shown. Images are representatives of three independent experiments. Scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12760) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunohistochemistry-NB600-362-img0011.jpg "Immunohistochemistry: HA Tag Antibody [NB600-362] - SUMO1 conjugates are not localized to amyloid plaques. (a) Sagittal brain sections of 24-week-old KI/AD and WT/AD animals were stained using anti-HA antibodies (red), 6E10 antibodies (green) that label amyloid beta 1-42 among other amyloid beta variants (epitope lies within amino acids 3-8 of amyloid beta) and anti-MAP2 antibodies (blue). Sections of the hippocampal subiculum are shown. Images are representatives of three independent experiments. Scale bar, 25 um. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12760) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: HA Tag Antibody [NB600-362] - Lysates of HEK293T human embryonic kidney cell line transfected with N-terminal HA-tagged MICA, N-terminal HA-tagged LGI-2, mock transfected, C-terminal HA-tagged LRRN5, and C-terminal HA-tagged Trance. PVDF membrane was probed with 1:500 dilution of 1:2500 dilution of goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1:5000 dilution of rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018).](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NB600-362-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-362] - HA-tagged proteins were detected in immersion fixed HEK293 human embryonic kidney cell line transfected with HA-tagged MGAT2 using 1 ug/mL goat anti-HA Tag polyclonal (NB600-362, Novus Biologicals), 1 ug/mL rabbit anti-HA Tag polyclonal (NB600-363, Novus Biologicals). Cells were stained using the appropriate secondary antibody, donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-362-img0005.jpg)
![Western Blot: HA Tag Antibody - BSA Free [NB600-362] - 200, 67, 22, 7, or 2 ng of E. coli whole cell lysateexpressing a multi-tag fusion protein. Antibodies: Affinitypurified, goat anti-HA antibody used for WBat 0.04 ug/ml (1:25,000). Detection: Chemiluminescencewith an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/HA-Tag-Antibody---BSA-Free-Western-Blot-NB600-362-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NB600-362] - HA tagged RFX6 overexpression in HEK293 stained with HA Tag antibody at a dilution of 1:250. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Immunocytochemistry-NB600-362-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-336] - Staining of K-562, U266, Daudi and normal T cells with c-Myc antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Immunocytochemistry-Immunofluorescence-NB600-336-img0010.jpg "Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-336] - Staining of K-562, U266, Daudi and normal T cells with c-Myc antibody. ICC/IF image submitted by a verified customer review.")
![Western Blot: c-Myc Antibody [NB600-336] - Detection of c-Myc in HtrA2-Myc Transfectant lysates .](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0012.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - Analysis of c-Myc on Jurkat whole cell extract using NB600-336.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0004.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - Lane 1: control HEK293 cell lysate. Lane 2: CMYC over-expression in HEK293 cell lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0014.jpg)
![Immunohistochemistry: c-Myc Antibody [NB600-336] - Analysis of c-Myc in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Immunohistochemistry-NB600-336-img0002.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - c-Myc in MOLT-4 cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0006.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-cMyc antibody (NB600-336) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of c-Myc antibody bound to the blotted protein. This c-Myc antibody detected the c-Myc specific band at its expected position (48-50kDa). The signal below 10 kDa in this blot is potentially the degraded protein and we have not characterized this band.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0013.jpg)
![Western Blot: c-Myc Antibody [NB600-336] - Analysis using the HRP conjugate of NB600-336. Detection of c-Myc in NIH/3T3 cell lysates (30ug and 45ug per lane) using anti-c-Myc antibody. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Western-Blot-NB600-336-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-336] - Detection of c-myc Tagged Plakoglobin by Immunofluorescence. Samples: Human microvascular endothelial cells expressing c-myc tagged plakoglobin following transient transfection.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Immunocytochemistry-Immunofluorescence-NB600-336-img0005.jpg)
![Simple Western: c-Myc Antibody [NB600-336] - Lane view shows a specific band for c-Myc in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Simple-Western-NB600-336-img0007.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Western-Blot-NB600-307-img0006.jpg "Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.")
![Immunohistochemistry: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Detection of Luciferase expression in CD34+ cells by Immunohistochemistry. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with monoclonal anti-Luciferase antibody. Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue. Nontransduced, cultured CD34+ cells were used as a negative control. Original magnification, 40X. Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102(10): 3478-3482, 2003.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Immunohistochemistry-NB600-307-img0004.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - WB analysis of NB600-307 on Luciferase Protein (NB810-74573)](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Western-Blot-NB600-307-img0005.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416171421.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - Induction of autophagy by TTFields is AMPK dependent.a U-87 MG & A172 cells either left untreated or treated w/ TTFields for indicated time points. Immunoblot analysis of GFP78 protein. Numeric values represent fold increase in GRP78 signal, normalized to loading control (GAPDH), relative to untreated control. b Quantification of intracellular ATP levels in U-87 MG cells either left untreated or treated w/ TTFields for 72 h.Results presented as average ATP concentration (nmol/2 × 106 cells) from 3 independent experiments (*P < 0.01, Student’s t-test). c U-87 MG & A172 cells either left untreated or treated w/ TTFields for indicated time points. Immunoblot analysis of pAMPK & pULK1proteins. GAPDH used as loading control. (5D-F) U-87 MG cells transfected w/ AMPK-targeting siRNA (siAMPK) or w/ siRNA sham vector (siVector), & incubated for 48 h w/ or w/out TTFields application. CQ 20 µM added for last 4 h of treatment where indicated. d (left panel) Immunoblot analysis of LC3 & AMPK. Numeric values represent fold-change in LC3-II signal, normalized to GAPDH signal, relative to respective control. d (right panel) CQ-treated cells fixed & stained for LC3 (green) & DAPI (blue), original magnifications: × 40. e Cell count of siAMPK- or siVector-expressing cells after TTFields treatment. (0.01 < *P < 0.05, Student’s t-test, n = 3). f siVector- & siAMPK-transfected U-87 MG cells either left untreated or treated w/ TTFields for 48 h. Cells then fixed & stained for cleaved caspase-3 (green) & DAPI (blue) (left panel). Images from each treatment analyzed manually & fraction of cleaved caspase-3-positive cells calculated for at least 200 cells from each group (right panel) (**P < 0.01, Student’s t-test, n = 2) Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241616016.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields induce autophagy in glioma cell lines.a U-87 MG & A172 cells were either left untreated or treated with TTFields at the last 24 h, 48 h, or 72 h of culturing. All cultures were plated on the same time, incubated overnight to allow cell attachment, & collected 72 h afterwards. Cells were collected, lysed, & samples were analyzed using immunoblotting for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, **P < 0.01, Student’s t-test). b Paraffin-embedded sections from sham- or TTFields-treated rats were stained with anti-LC3 Ab (green) & DAPI (blue). Representative images are presented. c Quantification of LC3 intensity, presented as fold increase from corresponding control (*P < 0.05, Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416163711.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - Immunofluorescence imaging of luciferase protein as a reporter of hybrid formation. Entire primary tumor & lung sections were imaged via tile scanning, & each image of the scan was carefully analyzed to confirm or refute positive staining for luciferase. The luciferase signal was considered a positive signal if it was above background levels associated with negative controls & corresponded to the cytoplasm of a cell with a nucleus. Rare luciferase-positive cells were detected in the primary tumors. Most red signal was not in the cytoplasm of cells associated with nuclei and, therefore, considered nonspecific [insets (a), (c)]. The lungs containing metastases on the other hand [(b), (d)] contained a large number of bona fide luciferase-positive cells corresponding to fusion products. Scale bars on slide scans = 100 μm. Scale bars on 40× inset = 25 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31069316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241618921.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields induce autophagy in glioma cell lines.a U-87 MG & A172 cells were either left untreated or treated with TTFields at the last 24 h, 48 h, or 72 h of culturing. All cultures were plated on the same time, incubated overnight to allow cell attachment, & collected 72 h afterwards. Cells were collected, lysed, & samples were analyzed using immunoblotting for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, **P < 0.01, Student’s t-test). b Paraffin-embedded sections from sham- or TTFields-treated rats were stained with anti-LC3 Ab (green) & DAPI (blue). Representative images are presented. c Quantification of LC3 intensity, presented as fold increase from corresponding control (*P < 0.05, Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416163757.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241616033.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416175224.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-380] - Detection of V5-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody NB600-380 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-380-img0005.jpg "Western Blot: V5 Epitope Tag Antibody [NB600-380] - Detection of V5-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody NB600-380 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.")
![ELISA: GFP Antibody (859) [NBP2-43575] - Coating Ab: mouse anti-GFP (2ug/well) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP (1:20000)](http://images.novusbio.com/fullsize/GFP-Antibody-859-ELISA-NBP2-43575-img0001.jpg "ELISA: GFP Antibody (859) [NBP2-43575] - Coating Ab: mouse anti-GFP (2ug/well) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP (1:20000)")
![Immunoprecipitation: GFP Antibody (859) [NBP2-43575] - Analysis of 400 ug cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 ug of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/GFP-Antibody-859-Immunoprecipitation-NBP2-43575-img0003.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [GT859] (NBP2-43575) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43575_mouse-monoclonal-gfp-antibody-859-western-blot-2611202518294921.jpg)
![GFP antibody [GT859] detects GFP protein by immunofluorescent analysis.Sample: GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Red: GFP stained by GFP antibody [GT859] (NBP2-43575) diluted at 1:2000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-43575_mouse-monoclonal-gfp-antibody-859-immunocytochemistry-immunofluorescence-261120251953519.jpg)
![Western Blot: c-Myc Antibody (9E11) [NB200-108] - Expression of cancer proliferation markers and in high SVCT-2 expression cell lines. Western blot analysis of c-Myc and cyclin D1 in Sw620 and Sw480 cell lines after co-treatment with L-ascorbic acid and phloretin. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29386-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Western-Blot-NB200-108-img0011.jpg "Western Blot: c-Myc Antibody (9E11) [NB200-108] - Expression of cancer proliferation markers and in high SVCT-2 expression cell lines. Western blot analysis of c-Myc and cyclin D1 in Sw620 and Sw480 cell lines after co-treatment with L-ascorbic acid and phloretin. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29386-7), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: c-Myc Antibody (9E11) [NB200-108] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB200-108, clone 9E11) at 1:600 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Immunohistochemistry-Paraffin-NB200-108-img0007.jpg)
![Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U937 cells with c-Myc [9E11] Antibody NB200-108AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Flow-Cytometry-NB200-108-img0014.jpg)
![Western Blot: c-Myc Antibody (9E11) [NB200-108] - Whole cell protein from PC3 (lane 1), U-2 OS (lane 2) and mouse testis (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-c-Myc in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Western-Blot-NB200-108-img0006.jpg)
![Western Blot: c-Myc Antibody (9E11) [NB200-108] - Expression of cancer proliferation markers in low SVCT-2 expressing cell lines. Expression of c-Myc and cyclin D1 was analyzed by western blotting in HCT15 and DLD-1 cell lines after L-ascorbic acid treatment. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29386-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Western-Blot-NB200-108-img0012.jpg)
![Western Blot: c-Myc Antibody (9E11) [NB200-108] - Expression of cancer proliferation markers and in high SVCT-2 expression cell lines. Western blot analysis of c-Myc and cyclin D1 in Sw620 and Sw480 cell lines after co-treatment with L-ascorbic acid and phloretin. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29386-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Western-Blot-NB200-108-img0013.jpg)
![Flow (Intracellular): c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E11) NB200-108AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Flow-Intracellular-NB200-108-img0008.jpg)
![Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E11) NB200-108PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Flow-Cytometry-NB200-108-img0009.jpg)
![Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - An intracellular stain was performed on U-937 cells with c-Myc Antibody [9E11] NB200-108 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/c-Myc-Antibody-9E11-Flow-Cytometry-NB200-108-img0010.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-344-img0009.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa.")
![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Analysis using the Agarose Immobilized conjugate of DYKDDDDK Epitope Tag Antibody [NB600-344]. DYKDDDK in transfected COS-7 cells using agarose immobilized DYKDDDDK Epitope Tag Antibody.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-344-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-344] - Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI and a PLA against the FLAG epitope (DYKDDDDK Epitope Tag Antibody [NB600-344]) was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122059), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-344-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-344-img0011.jpg)
![Proximity Ligation Assay: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344] - Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI & a PLA against the FLAG epitope was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25909512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-344_goat-dykddddk-epitope-tag-pab-bsa-free-31020241535645.jpg)
![Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - Analysis using the Biotin conjugate of NB100-62264. Detection of V5 tagged protein with Mouse anti V5-tag.](http://images.novusbio.com/fullsize/Paramyxovirus-SV5-Pk-Antibody-SV5-Pk1-Western-Blot-NB100-62264-img0004.jpg "Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - Analysis using the Biotin conjugate of NB100-62264. Detection of V5 tagged protein with Mouse anti V5-tag.")
![Sandwich ELISA: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - V5 tagged protein detected with Mouse anti V5-Tag.](http://images.novusbio.com/fullsize/Paramyxovirus-SV5-Pk-Antibody-SV5-Pk1-Sandwich-ELISA-NB100-62264-img0005.jpg)
![Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Western-Blot-NB600-326-img0001.jpg "Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).")
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0004.jpg)
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0005.jpg)
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0006.jpg)
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hVAV1-GST, E. coli (left) & hPARK2-GST-conditioned media (right) lysate(s).](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0007.jpg)