Strep II Tag Antibody (517) - Azide and BSA Free

Western Blot: Strep II Tag Antibody (517) [NBP2-43735] - Sample from human breast cell lysate (MDA-MB-231 cells). Absence of a band in the lane containing lysate from cells with empty vector confirm antibody is specific for Strep II Tag. Image submitted by a verified customer review.

Western Blot: Strep II Tag Antibody (517) [NBP2-43735] - PKN3 regulates cell migration in 3D environment of SrcF-transformed MEFs and tumor growth in vivo through interaction with p130Cas. Association of PKN3 with Src and Actin in p130Cas-dependent manner is shown by co-immunoprecipitations. SrcF-transformed cells with or without p130Cas were transfected by Strep-PKN3, and PKN3 was precipitated by Strep-Tactin(R) Superflow(R) resin followed by western blotting and detection by anti-StrepII antibody. Co-immunoprecipitated p130Cas, Src, and Actin were detected using appropriate antibodies (anti-p130Cas, anti-Src, anti-Actin). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/1878-0261.12401) licensed under a CC-BY license.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT517] (NBP2-43735) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Strep epitope Tag antibody [GT517] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT517] (NBP2-43735) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.

• Reactivity: ET
• Applications: WB, ICC/IF
• Clone: 517
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Strep II Tag Antibody (517) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Strep II Tag Antibody (517) - Azide and BSA Free (NBP2-43735) is a monoclonal antibody validated for use in WB and ICC/IF. Anti-Strep II Tag Antibody: Cited in 12 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Immunogen Amino acid sequence - ASWSHPQFEKGA
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Strep II Tag Antibody (NBP2-43735)(14)

Publications using NBP2-43735

• Jäntti N, Moreno-Layseca P, Chastney M et al. EPLIN alpha controls integrin recycling from Rab21 endosomes to drive breast cancer cell migration. Developmental cell 2025-07-09 [PMID: 40669465]
• Zhang H, Zhou C, Mohammad Z, Zhao J. Structural basis of human 20S proteasome biogenesis Nature Communications 2024-09-18 [PMID: 39294158]
• Dibus N, Salyova E, Kolarova K et al. FBXO38 is dispensable for PD-1 regulation EMBO Reports 2024-09-12 [PMID: 39266770]
• Nicolas Grosjean, Estella F. Yee, Desigan Kumaran, Kriti Chopra, Macon Abernathy, Sandeep Biswas, James Byrnes, Dale F. Kreitler, Jan-Fang Cheng, Agnidipta Ghosh, Steven C. Almo, Masakazu Iwai, Krishna K. Niyogi, Himadri B. Pakrasi, Ritimukta Sarangi, Hubertus van Dam, Lin Yang, Ian K. Blaby, Crysten E. Blaby-Haas A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria Nature Communications 2024-04-12 [PMID: 38609367]
• Mirco Glitscher, Inga Mareike Spannaus, Fabiane Behr, Robin Oliver Murra, Kathrin Woytinek, Daniela Bender, Eberhard Hildt The protease domain in HEV pORF1 mediates the replicase's localization to multivesicular bodies and its exosomal release. Cellular and molecular gastroenterology and hepatology 2024-01-06 [PMID: 38190941]
• Lee JD, Menasche BL, Mavrikaki M et al. Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53 Cell reports 2023-11-21 [PMID: 37991919] (WB)
• Ruiz D, Haynes C, Marable J et al. Development of OX40 agonists for canine cancer immunotherapy iScience 2022-10-21 [PMID: 36217551] (Western Blot, Mouse)
• Durkoop M Studying the immunomodulatory effects of SARS-CoV-2 viral proteins in human lung epithelial cell lines Front Immunol 2022-08-15 [PMID: 35967323]
• Marable J, Ruiz D, Jaiswal A Et al. Nanobody-based CTLA4 inhibitors for immune checkpoint blockade therapy of canine cancer patients Research Square 2021-07-31 [PMID: 34675296] (WB)
• Lidak T, Baloghova N, Korinek V et al. CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation International Journal of Molecular Sciences 2021-05-20 [PMID: 34065512] (ICC/IF)
• Hayn M, Hirschenberger M, Koepke L et al. Systematic Functional Analysis of SARS-CoV-2 Proteins Uncovers Viral Innate Immune Antagonists and Remaining Vulnerabilities Cell Reports 2021-04-01 [PMID: 33974846] (ICC/IF)
• Dibus M, BrAbek J, ROsel D A Screen for PKN3 Substrates Reveals an Activating Phosphorylation of ARHGAP18 International Journal of Molecular Sciences 2020-10-20 [PMID: 33092266]
• Gemperle J, Dibus M, Koudelkova L et al. The interaction of p130Cas with PKN3 promotes malignant growth Mol Oncol. [PMID: 30422386] (WB, Mouse)
• Margarido Galan D Investigating the immunomodulatory effects of SARS-CoV-2 proteins ORF3a, ORF6 and ORF9b on the inflammatory response of human lung epithelial cells Thesis 2022-01-01 (WB, Human)
  
  Details:
  Dilution used for WB 1:5000

Reviews for Strep II Tag Antibody (NBP2-43735) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/15/2018

Summary

• Application: Western Blot
• Sample Tested: Human breast cell lysate (MDA-MB-231),MDA MB 231 cells
• Species: Human
• Lot: 41246

reviewed by: Michal Dibus IP Human 05/26/2017

Summary

• Application: Immunoprecipitation
• Sample Tested: Immunoprecipitation from cell lysate,MDA-231 cell lysate,Western blot
• Species: Human
• Lot: 41246

Comments

• Comments: Immunoprecipitation of StrepII-tagged protein: MDA-MB-231 cells were transfected with StrepII-tagged protein or empty vector for control. For immunoprecipitation, 1ug of StrepII (517) antibody was crosslinked to Protein G sepharose with DMP (dimethyl pimelimidate) and incubated with cleared lysate in 4°C for 3h. For control, empty Protein G sepharose was used. The samples were resolved using SDS-PAGE and immunoblot with StrepII (517) antibody.