His Tag Antibody (AD1.1.10) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Dr, NASpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr
Clone
AD1.1.10
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

His Tag Antibody (AD1.1.10) [DyLight 405] Summary

Immunogen
PAX6 transcription factor linked to histidine tag
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
This 6-Histidine Epitope Tag Antibody (AD1.1.10) is useful for Western blot, Immunohistochemistry on frozen sections, ELISA, Flow Cytometry and Immunoprecipitation. This antibody is routinely tested in Western blot using Histidine-tagged recombinant proteins.

Reactivity Notes

This antibody is reactive to histidine tags.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for His Tag Antibody (AD1.1.10) [DyLight 405]

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Background

Epitope tagging is the procedure of inserting a short amino acid sequence into a protein within an expression vector via genetic engineering and it is a widely used technique in proteomics and various other biological sciences. The sequence of the tag is engineered using recombinant DNA technology, and grafted into the protein of interest by placing the encoded sequence of the epitope into a matching open reading frame. The antibody generated against this engineered epitope can recognize it, thereby enabling the tagged protein to be isolated, purified and visualized/analyzed by immunodetection methods. In other words, epitope tags represents an artificial epitope that is recognized by a common antibody and the antibodies recognizing a tag are useful for detecting proteins with low immunogenicity. 6-Histidine or H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate their purification and detection. Our 6-Histidine Epitope Tag antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells, and is useful for Western blot, IHC- paraffin embedded on frozen sections, ELISA, FLOW Cytometry and Immunoprecipitation applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-64768APC
DyLight 350 Labeled NB100-64768UV
DyLight 488 Labeled NB100-64768G
DyLight 550 Labeled NB100-64768R
DyLight 650 Labeled NB100-64768C
DyLight 680 Labeled NB100-64768FR
DyLight 755 Labeled NB100-64768IR
FITC Labeled NB100-64150
PE Labeled NB100-64151
PerCP Labeled NB100-64768PCP

Additional His Tag Products

His Tag NB100-64768V

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