His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal

Images

 
Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the ...read more
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the Biotin conjugate of NB100-64768. Detection of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with ...read more
Flow Cytometry: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the FITC conjugate of NB100-64768. Staining of Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) human peripheral blood lymphocytes, ...read more
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Drosophila, 20 ug whole cell lysate
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the HRP conjugate of NB100-64768. Detection of His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis of 1) 6x-His tagged FLCN protein and 2) 6x-His tagged homer protein.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, Simple Western, ELISA, Flow, IHC, IHC-Fr, In vitro, IP
Clone
AD1.1.10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Non-Recombinant Monoclonal
Concentration
1.0 mg/ml

Order Details

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His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal Summary

Immunogen
This His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal was developed against PAX6 transcription factor linked to histidine tag.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:250
  • ELISA 1:100 - 1:500
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry 1:100 - 1:300
  • Immunohistochemistry-Frozen 1:100 - 1:300
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
Application Notes
This antibody is routinely tested in WB using Histidine-tagged recombinant proteins. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-64768 in the following applications:

Publications
Read Publications using
NB100-64768 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

6x His Tag specific

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal

  • 6 His epitope tag
  • 6-His Tag
  • 6X His Tag
  • 6X His
  • 6x-His Tag
  • 6X-His
  • H
  • Hexa His tag
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • His6-Tag
  • polyhistidine tag
  • polyHistidine
  • Poly-histidine

Background

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V.,... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for His Tag Antibody (NB100-64768)(3)

Reviews for His Tag Antibody (NB100-64768) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Drosophila.

Reviews using NB100-64768:
Filter by Applications
WB
(2)
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(2)
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Images Ratings Applications Species Date Details
Western Blot His Tag NB100-64768
Enlarge
5
reviewed by:
Anonymous
WB Drosophila 07/10/2017
View

Summary

ApplicationWestern Blot
Sample TestedS2 lysate (with overexpressed recombinant protein)
SpeciesDrosophila
LotD-1
Western Blot His Tag NB100-64768
Enlarge
5
reviewed by:
Eric Wang
WB Drosophila 04/13/2017
View

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
SpeciesDrosophila
Lotc-4

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Other Available Formats

Additional His Tag Products

Array NB100-64768

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Recent Reviews

5
5
2
4
0
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0
2
0
1
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Anonymous
07/10/2017
Application: WB
Species: Drosophila

Eric Wang
04/13/2017
Application: WB
Species: Drosophila