Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the ...read more
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the Biotin conjugate of NB100-64768. Detection of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with ...read more
Flow Cytometry: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the FITC conjugate of NB100-64768. Staining of Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) human peripheral blood lymphocytes, ...read more
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Drosophila, 20 ug whole cell lysate
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the HRP conjugate of NB100-64768. Detection of His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis of 1) 6x-His tagged FLCN protein and 2) 6x-His tagged homer protein.
WB, Simple Western, ELISA, Flow, IHC, IHC-Fr, In vitro, IP
This antibody is routinely tested in WB using Histidine-tagged recombinant proteins. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Read 2 Reviews rated 5 using NB100-64768 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal
6 His epitope tag
6X His Tag
Hexa His tag
HHHHHH epitope tag
A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).
1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0
2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6
3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for His Tag Antibody (NB100-64768)(5)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 4 applications: ELISA, ICC/IF, IP, WB.
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