His Tag Antibody (AD1.1.10)

Images

 
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the Biotin conjugate of NB100-64768. Detection of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with ...read more
Flow Cytometry: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the FITC conjugate of NB100-64768. Staining of Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) human peripheral blood lymphocytes, ...read more
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Drosophila, 20 ug whole cell lysate
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the HRP conjugate of NB100-64768. Detection of His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis of 1) 6x-His tagged FLCN protein and 2) 6x-His tagged homer protein.
Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Dr, NASpecies Glossary
Applications WB, Simple Western, ELISA, Flow, IHC, IHC-Fr, In vitro, IP
Clone
AD1.1.10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

His Tag Antibody (AD1.1.10) Summary

Immunogen
PAX6 transcription factor linked to histidine tag
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:250
  • ELISA 1:100 - 1:500
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry 1:100 - 1:300
  • Immunohistochemistry-Frozen 1:100 - 1:300
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
Application Notes
This antibody is routinely tested in WB using Histidine-tagged recombinant proteins. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-64768 in the following applications:

Publications
Read Publications using
NB100-64768 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

This antibody is reactive to histidine tags.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for His Tag Antibody (AD1.1.10)

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Background

Epitope tagging is the procedure of inserting a short amino acid sequence into a protein within an expression vector via genetic engineering and it is a widely used technique in proteomics and various other biological sciences. The sequence of the tag is engineered using recombinant DNA technology, and grafted into the protein of interest by placing the encoded sequence of the epitope into a matching open reading frame. The antibody generated against this engineered epitope can recognize it, thereby enabling the tagged protein to be isolated, purified and visualized/analyzed by immunodetection methods. In other words, epitope tags represents an artificial epitope that is recognized by a common antibody and the antibodies recognizing a tag are useful for detecting proteins with low immunogenicity. 6-Histidine or H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate their purification and detection. Our 6-Histidine Epitope Tag antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells, and is useful for Western blot, IHC- paraffin embedded on frozen sections, ELISA, FLOW Cytometry and Immunoprecipitation applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for His Tag Antibody (NB100-64768)(2)

Reviews for His Tag Antibody (NB100-64768) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Drosophila.

Reviews using NB100-64768:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Drosophila
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot His Tag NB100-64768
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reviewed by:
Anonymous
WB Drosophila 07/10/2017
View

Summary

ApplicationWestern Blot
Sample TestedS2 lysate (with overexpressed recombinant protein)
SpeciesDrosophila
LotD-1
Western Blot His Tag NB100-64768
Enlarge
reviewed by:
Eric Wang
WB Drosophila 04/13/2017
View

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
SpeciesDrosophila
Lotc-4

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for His Tag Antibody (NB100-64768) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64768AF405
Alexa Fluor 488 Labeled NB100-64768AF488
Alexa Fluor 647 Labeled NB100-64768AF647
Alexa Fluor 700 Labeled NB100-64768AF700
Allophycocyanin Labeled NB100-64768APC
Biotin Labeled NB100-63172
DyLight 350 Labeled NB100-64768UV
DyLight 405 Labeled NB100-64768V
DyLight 488 Labeled NB100-64768G
DyLight 550 Labeled NB100-64768R
DyLight 650 Labeled NB100-64768C
DyLight 680 Labeled NB100-64768FR
DyLight 755 Labeled NB100-64768IR
FITC Labeled NB100-64768F
HRP Labeled NB100-63173
PE Labeled NB100-64151
PerCP Labeled NB100-64768PCP

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Recent Reviews

5
5
2
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2
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1
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Anonymous
07/10/2017
Application: WB
Species: Drosophila

Eric Wang
04/13/2017
Application: WB
Species: Drosophila