Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the Biotin conjugate of NB100-64768. Detection of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with ...read more
Flow Cytometry: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the FITC conjugate of NB100-64768. Staining of Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) human peripheral blood lymphocytes, ...read more
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Drosophila, 20 ug whole cell lysate
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the HRP conjugate of NB100-64768. Detection of His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.
Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis of 1) 6x-His tagged FLCN protein and 2) 6x-His tagged homer protein.
Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the ...read more
Hu, All-NA, DrSpecies Glossary
WB, Simple Western, ELISA, Flow, IHC, IHC-Fr, In vitro, IP
This antibody is routinely tested in WB using Histidine-tagged recombinant proteins. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Read 2 Reviews rated 5 using NB100-64768 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for His Tag Antibody (AD1.1.10)
6 His epitope tag
HHHHHH epitope tag
Epitope tagging is the procedure of inserting a short amino acid sequence into a protein within an expression vector via genetic engineering and it is a widely used technique in proteomics and various other biological sciences. The sequence of the tag is engineered using recombinant DNA technology, and grafted into the protein of interest by placing the encoded sequence of the epitope into a matching open reading frame. The antibody generated against this engineered epitope can recognize it, thereby enabling the tagged protein to be isolated, purified and visualized/analyzed by immunodetection methods. In other words, epitope tags represents an artificial epitope that is recognized by a common antibody and the antibodies recognizing a tag are useful for detecting proteins with low immunogenicity. 6-Histidine or H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate their purification and detection. Our 6-Histidine Epitope Tag antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells, and is useful for Western blot, IHC- paraffin embedded on frozen sections, ELISA, FLOW Cytometry and Immunoprecipitation applications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for His Tag Antibody (NB100-64768)(3)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 3 applications: ELISA, IP, WB.
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