His Tag Antibody (AD1.1.10) [DyLight 650]

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Product Details

Summary
Reactivity Hu, NASpecies Glossary
Applications WB, ELISA, Flow, IHC-Fr
Clone
AD1.1.10
Clonality
Monoclonal
Host
Mouse

His Tag Antibody (AD1.1.10) [DyLight 650] Summary

Immunogen
PAX6 transcription factor linked to histidine tag
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 650
Format
DyLight 650 (A=652, E=672)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry 1ul per million cells
  • Immunohistochemistry-Frozen
Application Notes
This 6-Histidine Epitope Tag Antibody (AD1.1.10) is useful for Western blot, Immunohistochemistry on frozen sections, ELISA, Flow Cytometry and Immunoprecipitation. This antibody is routinely tested in Western blot using Histidine-tagged recombinant proteins.

Reactivity Notes

This antibody is reactive to histidine tags.

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for His Tag Antibody (AD1.1.10) [DyLight 650]

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for His Tag Antibody (NB100-64768C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-64768APC
DyLight 350 Labeled NB100-64768UV
DyLight 405 Labeled NB100-64768V
DyLight 405LS Labeled NB100-64768V3
DyLight 488 Labeled NB100-64768G
DyLight 550 Labeled NB100-64768R
DyLight 680 Labeled NB100-64768FR
DyLight 755 Labeled NB100-64768IR
FITC Labeled NB100-64150
PE Labeled NB100-64151
PerCP Labeled NB100-64768PCP

Additional His Tag Products

His Tag NB100-64768C

Research Areas for His Tag Antibody (NB100-64768C)

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Blogs on His Tag

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Contact Information

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