| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Agarose | NBP1-97142 | |||
| Alkaline Phosphatase | NBP1-97220 | |||
| Biotin | NBP1-97078 | |||
| FITC | NBP1-96789 | |||
| HRP | NBP1-97073 | |||
| Rhodamine | NBP1-96790 | |||
| Texas Red | NBP1-97079 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-62622-0.1ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-16773 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Non-Recombinant Monoclonal | NB100-64768 | |||
| Non-Recombinant Monoclonal | NB100-64768SS | |||
| Azide and BSA Free | NBP2-80768 | |||
| BSA Free | NBP3-07011SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41076-0.025mg | |||
![Western Blot: VSV-G Epitope Tag Antibody [NB100-2485] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/VSV-G-Epitope-Tag-Antibody-Western-Blot-NB100-2485-img0001.jpg "Western Blot: VSV-G Epitope Tag Antibody [NB100-2485] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).")
![Immunocytochemistry/Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714] - HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA stained by HA tag antibody [4810] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-4810-Immunocytochemistry-Immunofluorescence-NBP2-43714-img0006.jpg "Immunocytochemistry/Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714] - HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA stained by HA tag antibody [4810] diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Human HEK293T cells overexpressed with Ly6K-HA. Dution 1:1000. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-4810-Western-Blot-NBP2-43714-img0005.jpg)
![Flow Cytometry: HA Tag Antibody (4810) [NBP2-43714] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted from a verified customer review.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-4810-Flow-Cytometry-NBP2-43714-img0004.jpg)
![Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of 293T whole cell lysate/extract A: 20 ug whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C: Immunoprecipitation of HA tagged protein by 2.5 ug of HA tagg antibody 10% SDS-PAGE. The immunoprecipitated HA tagged protein was detected by HA tag antibody [4810] diluted at 1:3000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-4810-Western-Blot-NBP2-43714-img0002.jpg)
![Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of A: 30 ug HeLa whole cell lysate/extract B: 30 ug whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [4810] dilution: 1:5000.](http://images.novusbio.com/fullsize/HA-Epitope-Tag-Antibody-4810-Western-Blot-NBP2-43714-img0003.jpg)
![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](http://images.novusbio.com/fullsize/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")
![SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - Lane 1: 5 uL Opal Prestained Marker Lane 2: Reduced Mouse IgG Whole Molecule (BULK ORDER) Lane 3: Reduced Mouse F(c) Fragment Lane 4: Reduced Mouse F(ab) Fragment Lane 5: Mouse IgM Kappa Myeloma Protein Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Mouse-IgG-Isotype-Control-SDS-Page-NBP1-97019-img0003.jpg)
![SDS-Page: Mouse IgG Isotype Control [NBP1-97019] - SDS-Page of Mouse IgG Isotype control. Lane 1: Mouse IgG reduced. Lane 2: Molecular Weight Marker. Lane 3: Mouse IgG non-reduced. Load: 1 ug per lane.Predicted/Observed size (non-reduced): 160 kDa, 160 kDa.Predicted/Observed size (reduced): 55 and 28 kDa, 55 and 28 kDa.](http://images.novusbio.com/fullsize/Mouse-IgG-Isotype-Control-SDS-Page-NBP1-97019-img0005.jpg)
![Simple Western: His Tag Antibody (359) [NBP2-43536] - Human purified protein. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Simple-Western-NBP2-43536-img0009.jpg "Simple Western: His Tag Antibody (359) [NBP2-43536] - Human purified protein. Simple Western image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (359) [NBP2-43536] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 6X His tag stained by 6X His tag antibody [359] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Immunocytochemistry-Immunofluorescence-NBP2-43536-img0013.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - 6x His antibody detects 6x His protein by Western blot analysis. A. Non-induced cell lysates from E. coli expressing HIS-tagged proteins. B. IPTG-induced cell lysates from E. coli expressing HIS-tagged proteins. 15 % SDS-PAGE. 6x His antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/His-Tag-Antibody-359-Western-Blot-NBP2-43536-img0011.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - Analysis of cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 ug of 6X His tag antibody [359] 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [359] diluted at 1:1000.](http://images.novusbio.com/fullsize/6-Histidine-Epitope-Tag-Antibody-359-Western-Blot-NBP2-43536-img0003.jpg)
![ELISA: His Tag Antibody (359) [NBP2-43536] - 6X His tag antibody [359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 ug/mL 6X His tag antibody [359] . Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates.](http://images.novusbio.com/fullsize/6-Histidine-Epitope-Tag-Antibody-359-ELISA-NBP2-43536-img0001.jpg)
![Western Blot: His Tag Antibody (359) [NBP2-43536] - 6x His antibody detects 6x His protein by Western blot analysis.Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12 % SDS-PAGE6x His antibody (NBP2-43536) dilution: 1:5000](http://images.novusbio.com/fullsize/nbp2-43536_mouse-monoclonal-his-tag-antibody-359-91020242075922.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT359] (NBP2-43536) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43536_mouse-monoclonal-his-tag-antibody-359-western-blot-261120251933919.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0001.jpg "Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of various amounts of E. coli lysate containing a multi-epitope tagged protein per well.")
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of His-tagged protein in E. coli lysates. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0012.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 680 conjugate of NBP2-31055. Detection of 6x-His Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0021.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0013.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 ug/mL).](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0014.jpg)
![Western Blot: His Tag Antibody (HIS.H8) [NBP2-31055] - Analysis of recombinant Treponema denticola major outer sheath His-tagged protein fragments (rMSP, rMSP-C, rMSP-V, rMSP-N) purified from E. coli was performed by loading 5 ug of per well. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Western-Blot-NBP2-31055-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: His Tag Antibody (HIS.H8) [NBP2-31055] - 6-Histidine Epitope Tag Antibody (HIS.H8) [NBP2-31055] - Analysis using the DyLight 488 conjugate of NBP2-31055. Staining of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a DyLight 488-conjugated 6x-His Epitope Tag monoclonal antibody at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken on a Thermo Scietific ArrayScan or ToxInsight Instrument at 20X magnification.](http://images.novusbio.com/fullsize/His-Tag-Antibody-HIS-H8-Immunocytochemistry-Immunofluorescence-NBP2-31055-img0024.jpg)
![Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Western-Blot-NB7588-img0006.jpg "Western Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7588-img0002.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of paraffin embedded human colonic tissue. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0005.jpg)
![Flow Cytometry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588]](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Flow-Cytometry-NB7588-img0004.jpg)
![Immunohistochemistry: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Analysis of mouse frozen tissue. Goat anti-Mouse Cy5 was used as a secondary antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Immunohistochemistry-NB7588-img0003.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Dots are Mouse IgG: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat anti-Mouse IgG Secondary Antibody (Pre-absorbed) Conjugate at 1ug/mL blocked for 1hr at RT. Imaged with BioRad ChemiDoc, Fluorescein filter. Image from the Cy5.5 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0008.jpg)
![Dot Blot: Goat anti-Mouse IgG F(ab')2 Secondary Antibody [NB7588] - Mouse IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-F-ab-2-Secondary-Antibody-Dot-Blot-NB7588-img0007.jpg)
![Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Western-Blot-NBP2-21581-img0017.jpg "Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.")
![Flow Cytometry: HA Tag Antibody [NBP2-21581] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HA-Tag-Antibody-Flow-Cytometry-NBP2-21581-img0013.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Use of DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] in Western Blot. Flag expression is significantly increased in 293 cells transfected with a flag-fusion protein. Image from a verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-29E4-G7-Western-Blot-NBP1-97410-img0009.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Use of DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] in Western Blot. Flag expression is significantly increased in 293 cells transfected with a flag-fusion protein. Image from a verified customer review.")
![Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Twenty-four clones were randomly selected and grown up from glycerol stocks by inoculating 0.5 mL 2X YT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 uL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and blotted using Mab DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410].](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-29E4-G7-Western-Blot-NBP1-97410-img0004.jpg)
![Western Blot: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Antibody detects both C-terminal linked and N-terminal linked FLAG tagged recombinant proteins.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-29E4-G7-Western-Blot-NBP1-97410-img0011.jpg)
![ELISA: DYKDDDDK Epitope Tag Antibody (29E4.G7) [NBP1-97410] - Detection of DYKDDDDK conjugated proteins using the DyLight 405 conjugated version of DYKDDDDK Epitope Tag Antibody (29E4.G7).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-29E4-G7-ELISA-NBP1-97410-img0010.jpg)
![Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. 2 ug His-recombinant protein B. 2 ug GST-recombinant protein C. 1 ug GST-recombinant protein D. 0.5 ug GST-recombinant protein 10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:5000](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-3G10-Western-Blot-NB600-446-img0002.jpg "Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. 2 ug His-recombinant protein B. 2 ug GST-recombinant protein C. 1 ug GST-recombinant protein D. 0.5 ug GST-recombinant protein 10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:5000")
![Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. Non-induced cell lysate from E. coli expressing GST-tagged proteinsB. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins10 % SDS-PAGE GST tag antibody [3G10] dilution: 1:1000](http://images.novusbio.com/fullsize/GST-Epitope-Tag-Antibody-3G10-Western-Blot-NB600-446-img0001.jpg)
![Western Blot: Strep II Tag Antibody [NBP2-41076] - Loading: A 5 ng and B 25 ng of StrepII-tagged recombinant protein. Antibodies: StrepII-tag 4337 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Strep-II-Tag-Antibody-Western-Blot-NBP2-41076-img0003.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: Strep II Tag Antibody [NBP2-41076] - Loading: A 5 ng and B 25 ng of StrepII-tagged recombinant protein. Antibodies: StrepII-tag 4337 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: Strep II Tag Antibody [NBP2-41076] - Human 293 cells whole cell lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Strep-II-Tag-Antibody-Western-Blot-NBP2-41076-img0002.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: Strep II Tag Antibody [NBP2-41076] - Analysis of (A) 5 and (B) 25 ng of StrepII-tagged recombinant protein with StrepII antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/Strep-II-Tag-Antibody-Western-Blot-NBP2-41076-img0001.jpg)