| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56095SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84578-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27335 | |||
| BSA Free | NB100-56599SS | |||
| Azide and BSA Free | NBP2-27335SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13732-25ul | |||
![Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/NEDD1-Antibody-7D10-Western-Blot-H00121441-M05-img0002.jpg "Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in NIH/3T3 ( Cat # L018V1 ).")
![Immunohistochemistry-Paraffin: NEDD1 Antibody (7D10) [H00121441-M05] - Analysis of monoclonal antibody to NEDD1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 0.75 ug/ml](http://images.novusbio.com/fullsize/NEDD1-Antibody-7D10-Immunohistochemistry-Paraffin-H00121441-M05-img0005.jpg)
![Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/NEDD1-Antibody-7D10-Western-Blot-H00121441-M05-img0001.jpg)
![ELISA: NEDD1 Antibody (7D10) [H00121441-M05] - Detection limit for recombinant GST tagged NEDD1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEDD1-Antibody-7D10-ELISA-H00121441-M05-img0007.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.Lane 1: ENO1 transfected lysate(47 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Western-Blot-H00002023-M01-img0015.jpg "Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.Lane 1: ENO1 transfected lysate(47 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Immunocytochemistry-Immunofluorescence-H00002023-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Immunohistochemistry-Paraffin-H00002023-M01-img0010.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 transfected lysate using anti-ENO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ENO1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Western-Blot-H00002023-M01-img0011.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - ENO1 monoclonal antibody (M01), clone 8G8 Analysis of ENO1 expression in MCF-7.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Western-Blot-H00002023-M01-img0013.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - ENO1 monoclonal antibody (M01), clone 8G8. Analysis of ENO1 expression in human colon.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Western-Blot-H00002023-M01-img0014.jpg)
![ELISA: Enolase 1 Antibody (8G8) [H00002023-M01] - Detection limit for recombinant GST tagged ENO1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-ELISA-H00002023-M01-img0016.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - LHPP suppresses aerobic glycolysis. Protein expression levels of Enolase 1 in LHPP-overexpressing or LHPP-knockdown MGC-803 cells and control cells on western blot. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35568711/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/h00002023-m01_mouse-monoclonal-enolase-1-antibody-8g8-25520231539522.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - LHPP suppresses aerobic glycolysis.A Relative mRNA expression levels of glycolytic genes & glutamine transporters in LHPP-overexpressing or LHPP-knockdown MGC-803 cells & control cells by qPCR. B Protein expression levels of glycolytic genes & glutamine transporters in LHPP-overexpressing or LHPP-knockdown MGC-803 cells & control cells on western blot. C IHC staining of LHPP & HIF1A in TMAs & their correlation. Scale bars = 100 μm. D Oxygen consumption rate & extracellular acidification rate of LHPP-overexpressing or LHPP-knockdown MGC-803 cells & control cells were measured using the Seahorse Bioscience XF96 analyser. E Human phosphokinase microarray assay analysis of the conditioned medium from stably transfected HGC-27 cells. A summary of the relative signal intensities of the indicated proteins is shown. G, H IHC staining of LHPP & p-GSK3b in TMAs & their correlation. Scale bars = 200 μm. F Combined LHPP & GSK3b by co-immunoprecipitation. I Protein expression levels of the Wnt pathway in LHPP-overexpressing or LHPP-knockdown MGC-803 cells & control cells on western blot. Data were analyzed using the Wilcoxon test. *P < 0.05, **P < 0.01, ***P < 0.001, KEGG Kyoto encyclopaedia of genes & genomes, GO gene ontology, qPCR quantitative polymerase chain reaction, IHC immunohistochemical, TMA tissue microarray. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35568711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00002023-m01_mouse-monoclonal-enolase-1-antibody-8g8-310202415482282.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0034.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunohistochemistry-Paraffin-NB100-464-img0029.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0032.jpg)
![Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 (lot 2) used for IP at 6 ug/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated Chk1, NB100-464 (lot 2) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunoprecipitation-NB100-464-img0033.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells.(G) WB for indicated proteins in clones indicated on the top. Darker exposure of ORC2 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415371972.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC1 in HCT116 p53-/- cells.(A) Strategy for insertion of a blasticidin gene & poly A site after first methionine of ORC1 in the second exon. (B) PCR on genomic DNA of indicated clones. WT: HCT116 p53-/- & ORC1+/+. ORC1 Knockout clones, B14, B48 & BP32 have an insert on both alleles of ORC1 as indicated by the absence of 0.6 kb PCR product. (C) Western blot for indicated proteins in clones indicated on the top. Darker exposure of the ORC1 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. (D) Immunoblot of soluble & chromatin-associated proteins in the clones indicated at the top. For each panel, all the lanes are from the same blot & exposure. (E) Input cell lysate & immunoprecipitates of ORC1 immunoblotted for ORC1. Darker exposure of the top blots is shown in the middle. Tubulin in the cell lysate or the IgG band in the immunoprecipitate serves as loading control.DOI://dx.doi.org/10.7554/eLife.19084.005 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415525253.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Total protein from human THP1, A431, A549 and U2OS cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:2000 dilution of anti-BAX in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0006.jpg "Western Blot: Bax Antibody [NB100-56095] - Total protein from human THP1, A431, A549 and U2OS cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:2000 dilution of anti-BAX in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence")
![Immunohistochemistry-Paraffin: Bax Antibody [NB100-56095] - Formalin-fixed, paraffin-embedded sections of human colon cancer fom a tissue microarray stained for BAX using NB100-56095 at 1:2000. A. High Bax expression. B. Low Bax expression. Hematoxylin-eosin counterstain](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NB100-56095-img0007.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Analysis of BAX in colon tumor cell lines using this antibody. Bax is detected in the SW620 and DLD-1 cell lines. However, Bax was not detected in the cell lines (LoVo, DU145, and LS180) known to have frameshift mutations in Bax (Rampino et al. 1997). An antibody to F1ATPase was used as a protein loading control.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0004.jpg)
![Western Blot: Bax Antibody [NB100-56095] - Analysis of Bax in Daoy whole cell lysate using anti-Bax antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bax-Antibody-Western-Blot-NB100-56095-img0005.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining in human skin and skeletal muscle tissues . Corresponding CD44 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining in human skin and skeletal muscle tissues . Corresponding CD44 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP1-84578] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84578-img0022.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human lymph node shows strong positivity in plasma membrane in non-germinal center cells.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0023.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0018.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human squamous cell lung carcinoma shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0019.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0021.jpg)
![Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-1556-img0023.jpg "Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review")
![Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunocytochemistry-NB100-1556-img0024.jpg)
![Immunohistochemistry: NONO Antibody [NB100-1556]](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-NB100-1556-img0026.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NONO Antibody [NB100-1556] - Detection of Human NONO on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lystate (50 microgram) using NB100-1556. NONO was also immunoprecipitated using anti-NONO antibody NB100-1554 or NB100-1555 at 3 microg/mg lysate.](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-1556-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NONO Antibody [NB100-1556] - Whole cell lysate NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-1556-img0018.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-1556-img0020.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Affinity purified rabbit anti-NONO used at a dilution of 1:1,000 (0.2 ug/ml).](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-1556-img0025.jpg)
![Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in human colon.](http://images.novusbio.com/fullsize/ERCC6L-Antibody-Western-Blot-H00054821-D01P-img0004.jpg "Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in human colon.")
![Immunocytochemistry/Immunofluorescence: ERCC6L Antibody [H00054821-D01P] - IF analysis of ERCC6L in mouse and human cell lines. Image courtesy of product review submitted by Corentin Laulier.](http://images.novusbio.com/fullsize/ERCC6L-Antibody-Immunocytochemistry-Immunofluorescence-H00054821-D01P-img0002.jpg)
![Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in transfected 293T cell line by ERCC6L polyclonal antibody.Lane 1: ERCC6L transfected lysate(46.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ERCC6L-Antibody-Western-Blot-H00054821-D01P-img0005.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Western-Blot-NBP2-24695-img0004.jpg)
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-24695-img0005.jpg)
![Western Blot: RNA Helicase A Antibody [NB110-40579] - The identification of host proteins immunoprecipitated with L1 ORF1p-FLAG. Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Primary antibodies used to probe western blots are indicated to the left of the images (ahnRNPL (NBP1-67852), aILF3 (EPR3627), aLARP1 (NBP1-19128), aMATR3 (NB100-1761), aNCL (NB100-1920)). Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005121), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Western-Blot-NB110-40579-img0015.jpg "Western Blot: RNA Helicase A Antibody [NB110-40579] - The identification of host proteins immunoprecipitated with L1 ORF1p-FLAG. Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Primary antibodies used to probe western blots are indicated to the left of the images (ahnRNPL (NBP1-67852), aILF3 (EPR3627), aLARP1 (NBP1-19128), aMATR3 (NB100-1761), aNCL (NB100-1920)). Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005121), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RNA Helicase A Antibody [NB110-40579] - Immunocytochemistry of KYSE-30 cells to evaluate colocalization of RNA-helicase A (NDHII, green) and RCC2 (red). Colocalization signal is detected in nucleolus. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40579-img0014.jpg)
![Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NB110-40579] - FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-DHX9 used at a dilution of 1:200 (1 ug/mL). Detection: DAB](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Immunohistochemistry-NB110-40579-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Western-Blot-NB110-40579-img0012.jpg)
![Immunoprecipitation: RNA Helicase A Antibody [NB110-40579] - Detection of human DHX9 by Western blot of immunoprecipitates. Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 (lot NB110-40579-2) used for IP at 6 ug per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody NB110-40579-1. For blotting immunoprecipitated DHX9, NB110-40579 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RNA-Helicase-A-Antibody-Immunoprecipitation-NB110-40579-img0013.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Immunocytochemistry-Immunofluorescence-NB100-56599-img0005.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0003.jpg)
![Western Blot: PARP Antibody [NBP2-13732] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARP-Antibody-Western-Blot-NBP2-13732-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PARP Antibody [NBP2-13732] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP2-13732] - Staining of human cell line HEK 293 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13732-img0007.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0014.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human lymph node shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0010.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human pancreas shows strong nuclear positivity.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0011.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human parathyroid gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0012.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0013.jpg)
![Western Blot: EIF3A Antibody [NBP1-18891] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. eIF3A/EIF3S10 was also immunoprecipitated by rabbit antieIF3A/ EIF3S10 antibody NBP1-18892.](http://images.novusbio.com/fullsize/EIF3A-Antibody-Western-Blot-NBP1-18891-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: EIF3A Antibody [NBP1-18891] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. eIF3A/EIF3S10 was also immunoprecipitated by rabbit antieIF3A/ EIF3S10 antibody NBP1-18892.")
