| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05423-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22124SS | |||
| Azide and BSA Free | NBP2-80558 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80819 | |||
| BSA Free | NB100-507SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33148 | |||
| IHC-Prediluted | NBP2-44386 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-59724SS | |||
| Azide and BSA Free | NBP2-80643 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22113SS | |||
| Azide and BSA Free | NBP2-80737 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77455SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68281SS | |||
| Azide and BSA Free | NBP2-80993 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54791SS | |||
| Azide and BSA Free | NBP2-80822 | |||
![Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NBP1-05423-img0003.jpg "Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - Analysis of mixed fibroblast and PC12 pheochromocytoma cell culture stained with chicken pAb to peripherin, NBP1-05423, dilution 1:5,000 in green, and costained with rabbit pAb to vimentin, RPCA-Vim, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. NBP1-05423 antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0005.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (red) in nerve bundles in the human larynx. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - shows rat mixed neuron/glial cultures stained with NBP1-05423, our chicken polyclonal antibody to peripherin (green channel) and rabbit antibody to the neurofilament subunit alpha-internexin NBP1-05207 (green channel). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and alpha-internexin, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0001.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (green) in the mouse brainstem including the spinal trigeminal tract (right side of image) and arching fiber bundle to the solitary nuclear tract (center). Nissl Counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0006.jpg)
![Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-Western-Blot-NBP2-22124-img0006.jpg "Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.")
![Immunocytochemistry/Immunofluorescence: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-22124-img0004.jpg)
![Immunohistochemistry-Paraffin: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-22124-img0005.jpg)
![Flow Cytometry: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of HepG2 cells using ABCG2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-Flow-Cytometry-NBP2-22124-img0003.jpg)
![ELISA: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-ELISA-NBP2-22124-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Mouse Monoclonal ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Human brain microvascular endothelial cells stained for cilia (red) and ABCG2 (green) and nuclear DAPI (blue). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-22124_mouse-monoclonal-abcg2-cd338-antibody-3g8-immunocytochemistry-immunofluorescence-612025101023..jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW128) at 5 ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Immunocytochemistry-Immunofluorescence-NB100-507-img0005.jpg "Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW128) at 5 ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.")
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Detection of p300 in a HeLa nuclear extract using NB100-507 (1:250). ECL: 15 sec exposure.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0003.jpg)
![Flow (Intracellular): KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on THP-1 cells with KAT3B/p300 (RW128) antibody NB100-507 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Flow-Intracellular-NB100-507-img0004.jpg)
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Subcellular fractionation and localization of Src and p300 in Panc-1 cells. Cell lysates of equal total protein or Panc-1 subcellular fractions were subjected to immunoblotting analysis for Src, pSrc, p300, tubulin (cytoskeletal proteins), GAPDH (soluble cytoplasmic proteins), calnexin (endoplasmic reticulum), grim19 (mitochondria), histone H3 (chromatin), and lamin A/C (nuclear lamina). Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW128) NB100-507 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Immunocytochemistry-Immunofluorescence-NB100-507-img0009.jpg)
![Flow Cytometry: KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on Raw264.7 cells with KAT3B/p300 Antibody (RW128) NB100-507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Flow-Cytometry-NB100-507-img0010.jpg)
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Src and p300 co-association in Panc-1 cells. Immunoblotting analysis of Src or p300 immunecomplexes from Panc-1 nuclear isolates probing for Src or p300, or IgG control. Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0006.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0011.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A/MART-1 Antibody (M2-7C10).")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-fixed, paraffin-embedded human skin stained with MART-1 Monoclonal Antibody (M2-7C10).](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0007.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0005.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Formalin-paraffin human melanoma stained with MART-1 Ab (M2-7C10). Note cytoplasmic staining of cells.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Paraffin-NBP2-15197-img0006.jpg)
![Immunohistochemistry-Frozen: Melan-A/MART-1 Antibody (M2-7C10) [NBP2-15197] - Analysis using the Alexa-Fluor(R)-488 conjugate for this antibody. Melan-A (green) was detected in human skin (melanoma) using Melan-A-AlexaFluor488 antibody (1:40; 1 hour) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-M2-7C10-Immunohistochemistry-Frozen-NBP2-15197-img0010.jpg)
![Western Blot: CD36 Antibody (D-2712) [NB110-59724] - Detection of CD36 in mouse adipose lysate.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-D-2712-Western-Blot-NB110-59724-img0007.jpg "Western Blot: CD36 Antibody (D-2712) [NB110-59724] - Detection of CD36 in mouse adipose lysate.")
![Immunohistochemistry: CD36 Antibody (D-2712) [NB110-59724] - Staining of CD36 in mouse liver.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-D-2712-Immunohistochemistry-NB110-59724-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and permeabilized using PBS with 0.1% Triton X-100 before incubating them with Fibronectin antibody or the mouse IgG1 isotype control antibody. DyLight 488 conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody was used at 1: 200 dilution to detect the antigen-primary antibody complexes and the cells were counterstained with DAPI for labeling the nuclei. This Fibronectin antibody generated the expected punctate cytoplasmic-membranous staining and the signal was localized more to the inter-cellular spaces in clustered cells.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Immunocytochemistry-Immunofluorescence-NBP2-22113-img0009.jpg "Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and permeabilized using PBS with 0.1% Triton X-100 before incubating them with Fibronectin antibody or the mouse IgG1 isotype control antibody. DyLight 488 conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody was used at 1: 200 dilution to detect the antigen-primary antibody complexes and the cells were counterstained with DAPI for labeling the nuclei. This Fibronectin antibody generated the expected punctate cytoplasmic-membranous staining and the signal was localized more to the inter-cellular spaces in clustered cells.")
![Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa (1) and HepG2 (2) whole cell lysates using Fibronectin antibody (2F4) at 2 ug/mL.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Western-Blot-NBP2-22113-img0008.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded stomach cancer tissues using Fibronectin mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Immunohistochemistry-Paraffin-NBP2-22113-img0006.jpg)
![Flow Cytometry: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa cells using Fibronectin mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Flow-Cytometry-NBP2-22113-img0004.jpg)
![Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against human Fibronectin (AA: 1965-2176) recombinant protein. Expected MW is 49.6 kDa.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Western-Blot-NBP2-22113-img0002.jpg)
![Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against HEK293 (1) and Fibronectin (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Western-Blot-NBP2-22113-img0003.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded lung cancer tissues using Fibronectin mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Immunohistochemistry-Paraffin-NBP2-22113-img0005.jpg)
![ELISA: Fibronectin Antibody (2F4) [NBP2-22113] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng).](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-ELISA-NBP2-22113-img0001.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0023.jpg "Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-NB300-750-img0024.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0017.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of various human, mouse and non-human primate whole cell lysates.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0016.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0019.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of Pax6 in skate (Leucoraja erinacea) brain lysate using anti-Pax6 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NB300-750-img0011.jpg)
![Immunoprecipitation: Pax6 Antibody [NB300-750] - Analysis of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500ug whole cell lysate with 5ug of PAX6 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunoprecipitation-NB300-750-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs).(a) Schematic description of the two-stage protocol applied to iPSC-renal commitment. (b, c, d, e) Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. (b) The pluripotency markers SSEA4, TRA-1-81, Nanog, ME marker T(Bry) & IM marker Osr1. (c) IM & MM marker expression as Wt1, Pax8, Pax2, Six2 & Sall1. (d) Renal progenitor markers CD133, CD24, NCAM, glomerular epithelial marker Claudin1 & proximal tubular epithelial markers AQP1, GGT1. (e) Markers identifying endodermal AFP, ectodermal Pax6, & cardiac mesodermal Nkx2.5 derivation. Nuclei are stained with DAPI (blue). Scale bars: 20 μm (b, c, d, e). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-750_rabbit-polyclonal-pax6-antibody-3102024161898.jpg)
![Immunohistochemistry-Paraffin: pan Cadherin Antibody [NB200-592] - Mouse cervical cancer tissue stained with N-Cadherin antibody (green). Nuclei were counterstained with DAPI (Blue). Antigen retrieval was done with pepsin treatment. Image from verified customer review.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NB200-592-img0003.jpg "Immunohistochemistry-Paraffin: pan Cadherin Antibody [NB200-592] - Mouse cervical cancer tissue stained with N-Cadherin antibody (green). Nuclei were counterstained with DAPI (Blue). Antigen retrieval was done with pepsin treatment. Image from verified customer review.")
![Western Blot: pan Cadherin Antibody [NB200-592] - Whole extract of Chicken Cardiac Muscle were separated on SDS-PAGE and probed with Anti-Pan Cadherin antibody produced in Rabbit. The antibody was developed using 1:1000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:200 antibody2. 1:400 antibody3. Negative Control](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-Western-Blot-NB200-592-img0005.jpg)
![Immunohistochemistry: pan Cadherin Antibody [NB200-592] - Immunoperoxidase staining of a formalin-fixed, paraffin-embedded section of human heart using Rabbit Anti-Pan Cadherin.](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-Immunohistochemistry-NB200-592-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP1-77455] - HSP70 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77455-img0009.jpg "Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP1-77455] - HSP70 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77455] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunohistochemistry-NBP1-77455-img0010.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on Jurkat cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Flow-Cytometry-NBP1-77455-img0014.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - Single cell suspension generated from B16-F10 tumor excised from C57BL/6 Mouse stained with Hsp70 & FITC secondary (blue) or unstained (red). [Image provided by a varified research professional; see Product Reviews for details on protocol employed].](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Flow-Cytometry-NBP1-77455-img0011.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on A549 cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Flow-Cytometry-NBP1-77455-img0013.jpg)
![Simple Western: HSP70/HSPA1A Antibody [NBP1-77455] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Simple-Western-NBP1-77455-img0012.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0012.jpg "Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.")
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0011.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0003.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0005.jpg)
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0009.jpg)
![Simple Western: TRF-1 Antibody (57-6) [NB110-68281] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Simple-Western-NB110-68281-img0010.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) antagomiR in rat vascular smooth muscle cells (A7r5) induced angiogenesis & proliferation but suppressed senescence. a Schematic of the protocol for transfection of A7r5 VSMCs with a miRNA antagomiR control (antagomiR NC) or a miR-214 antagomiR & processed at the indicated times. b The effects of miR-214 antagomiR on VSMC cell proliferation were tested by CCK-8 assay. c Angiogenetic mRNA expression levels of NOS3, VEGFA, CXCL12 & CXCR4 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). d Senescence-associated mRNA expression of TERT, TERF1 & TERF2 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). e, G Representative western blots depicting TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking expression in antagomiR NC or miR-214 antagomiRs transfected cells. f, h Normalized expression of TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking (n = 3). i Senescence-associated beta -galactosidase staining demonstrating senescence in SCR or miR-214 transfected cells. j Bar graphs show quantification of relative of SA-beta -gal positive cells (n = 3). Data are presented as the means ± SEM. * P < 0.05; ** P < 0.01; ns, non-significance (Two-tailed Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-68281_mouse-monoclonal-trf-1-antibody-57-6-310202416212346.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Ki67/MKI67 Antibody (1297A) NBP2-54791 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunocytochemistry-Immunofluorescence-NBP2-54791-img0007.jpg "Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Ki67/MKI67 Antibody (1297A) NBP2-54791 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in Ki67/MKI67 knockout (KO) HeLa cell line using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # NBP2-54791) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Knockout-Validated-NBP2-54791-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-54791 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunocytochemistry-Immunofluorescence-NBP2-54791-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Ki67/MKI67 Antibody [1297A] conjugated to Alexa Fluor 647 (NBP2-54791AF647) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunocytochemistry-Immunofluorescence-NBP2-54791-img0010.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki-67/MKI67 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Ki-67/MKI67 Monoclonal Antibody at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunohistochemistry-Paraffin-NBP2-54791-img0002.jpg)
![Flow (Intracellular): Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki-67/MKI67 Antibody (1297A) [NBP2-54791] - An intracellular stain was performed on Jurkat Cells with Ki-67/MKI67 (1297A) antibody NBP2-54791 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Flow-Intracellular-NBP2-54791-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Ki67/MKI67 (1297A) at 2.0 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Immunocytochemistry-Immunofluorescence-NBP2-54791-img0003.jpg)
![Flow Cytometry: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - An intracellular stain was performed on HeLa cells with Ki67/MKI67 Antibody [1297A] NBP2-54791APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-1297A-Flow-Cytometry-NBP2-54791-img0005.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki67/MKI67 immunoreactivity in mouse small intestine. NBP2-54791 was diluted to 0.5ug/mL in Antibody Diluent and was left on sections for 1h at room temperature. Secondary was Horse Anti-Rabbit HRP. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-54791_rabbit-monoclonal-ki67-mki67-antibody-1297a-1552023121119.jpg)
![Immunohistochemistry-Paraffin: Rabbit Monoclonal Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki67 immunoreactivity in FFPE sections of human tonsil. NBP2-54791 was used at 500ng per mL and was left on tissue sections for 1hr at room temperature. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-54791_rabbit-monoclonal-ki67-mki67-antibody-1297a-immunohistochemistry-paraffin-15202512223..jpg)
![Western Blot: KAT3B/p300 Antibody [NB500-161] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1alpha, V5, and p300. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/7142), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0012.jpg "Western Blot: KAT3B/p300 Antibody [NB500-161] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1alpha, V5, and p300. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/7142), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunohistochemistry-Paraffin-NB500-161-img0008.jpg)
![Western Blot: KAT3B/p300 Antibody [NB500-161] - Analysis of HeLa lysates using NB500-161. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: KAT3B/p300 Antibody [NB500-161] - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0010.jpg)
![KAT3B/p300 Antibody [NB500-161] - Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for IP at 6 ug/mg lysate. p300 was also immunoprecipitated by a previous lot (lot NB500-161-1) of this antibody. For blotting immunoprecipitated p300, NB500-161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-NB500-161-img0011.jpg)