| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-22711SS | |||
| Azide and BSA Free | NBP2-80859 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-60046SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1832SS | |||
| Azide and BSA Free | NBP2-80962 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-64979-0.05mg | |||
| Azide Free | NB100-64979SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22124SS | |||
| Azide and BSA Free | NBP2-80558 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-137-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87102-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Non-Recombinant Monoclonal | NB100-64768SS | |||
![Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MUC1 [SM3] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MUC-1-Antibody-SM3-Immunocytochemistry-Immunofluorescence-NB120-22711-img0004.jpg "Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MUC1 [SM3] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.](http://images.novusbio.com/fullsize/MUC-1-Antibody-SM3-Immunohistochemistry-Paraffin-NB120-22711-img0003.jpg)
![Flow Cytometry: MUC1 Antibody (SM3) - BSA Free [NB120-22711] - An intracellular stain was performed on MCF7 cells with MUC1 Antibody (SM3) NB120-22711AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/MUC1-Antibody-SM3-BSA-Free-Flow-Cytometry-NB120-22711-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MUC-1 antibody was tested in MCF7 cells at 10 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.](http://images.novusbio.com/fullsize/MUC-1-Antibody-SM3-Immunocytochemistry-Immunofluorescence-NB120-22711-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong diffused immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.](http://images.novusbio.com/fullsize/MUC-1-Antibody-SM3-Immunohistochemistry-Paraffin-NB120-22711-img0002.jpg)
![Western Blot: MUC-1 Antibody [NBP1-60046] - Total protein from mouse 3T3 cells and human HeLa cells, stomach and colon was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MUC-1 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MUC-1-Antibody-Western-Blot-NBP1-60046-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MUC1 Antibody [NBP1-60046] - Tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Cells were counterstained for alpha-tubulin against DyLight 550 (Red) and mounted in DAPI fluoromount (Blue).](http://images.novusbio.com/fullsize/MUC-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-60046-img0010.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Human kidney lysate tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/MUC1-Antibody-Immunohistochemistry-Paraffin-NBP1-60046-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Pig stomach.](http://images.novusbio.com/fullsize/MUC1-Antibody-Immunohistochemistry-Paraffin-NBP1-60046-img0007.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Pig stomach.](http://images.novusbio.com/fullsize/MUC1-Antibody-Immunohistochemistry-Paraffin-NBP1-60046-img0008.jpg)
![Immunohistochemistry-Paraffin: MUC1 Antibody [NBP1-60046] - Human stomach.](http://images.novusbio.com/fullsize/MUC1-Antibody-Immunohistochemistry-Paraffin-NBP1-60046-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: MUC1 Antibody - BSA Free [NBP1-60046] - Dexamethasone induces Muc1-CT nuclear translocation & co-localization with GR alpha in bronchial epithelium of WT mice but not in MUC1 KO mice. C57BL/6 Muc1 KO mice & WT mice were undergoing dexamethasone 10 mg/kg/day (orally) for 6 days. Animals were sacrificed at day 6 & lungs were fixed in paraformaldehyde (4%) for 48 h & embedded in Tissue-Tek® OCT™ cryosectioning compound. Lung slices were immunostained with MUC1-cytoplasmic tail (CT) & glucocorticoid receptor alpha (GR alpha ) antibodies with rhodamine/fluorescein secondary antibodies. DAPI was used to mark cell nucleus. Representative con-focal images are showed. Scale bar: 5 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30458870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-60046_rabbit-polyclonal-muc1-antibody-310202415363741.jpg)
![Chromatin Immunoprecipitation: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 binds RIPK1 to mediate the RIPK1/RIPK3 pathway. (a,b) MUC1 knockdown by MUC1‐siRNA or NC‐siRNA were assayed in 16HBE cells following stimulation with TNF‐ alpha (300 ng/ml). After 24 hr of stimulation, RIPK3 & RIPK1 protein levels were measured by western blot analysis. (c,d) Normal 16HBE cells were treated with TNF‐ alpha for 24 hr, & the interaction between MUC1‐CT & RIPK1/RIPK3 were confirmed by immunoprecipitation. Data are expressed as means ± SD & were analyzed by one‐way analysis of variance, *p < 0.05. NC group (transfected with NC siRNA); MUC1‐siRNA group (transfected with MUC1‐siRNA). 16HBE: human bronchial epithelial; IB: immunoblotting; IP: immunoprecipitation; RIPK1: receptor‐interacting protein kinase‐1; RIPK3: receptor‐interacting protein kinase‐3; NC: negative control; siRNA: small interfering RNA; TNF‐ alpha : tumor necrosis factor‐ alpha Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30666647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-60046_rabbit-polyclonal-muc1-antibody-310202416161843.jpg)
![Western Blot: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 downregulation & TLR4 overexpression in lung tissue from heavy smokers & COPD patients. Lung tissue from healthy (n = 10), smokers (n = 11) & COPD patients (n = 13) were analysed. MUC1 mRNA gene expression in lung tissue homogenates (a), bronchial epithelial cells (b) & sputum neutrophils (c). d MUC1-CT protein expression in lung homogenates.TLR4 mRNA gene expression in lung tissue homogenates (e), bronchial epithelial cells (f) & sputum neutrophils (g). h MUC1-CT protein expression in lung homogenates. i Correlation of TL4 & MUC1 gene expression in lung tissue from smokers & COPD patients. j Correlation of FEV1% & MUC1 gene expression in lung tissue from smokers & COPD patients. Gene expression was analyzed by real time PCR using the 2-delta Ct as described in methods. Protein expression was analyzed by western blot. Representative western blot are showed. Data are presented as median with interquartile range. “P” exact values were obtained following Kruskal Wallis test. FEV1%: forced expiratory volume in 1 s Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30458870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-60046_rabbit-polyclonal-muc1-antibody-310202416165614.jpg)
![Chromatin Immunoprecipitation: MUC1 Antibody - BSA Free [NBP1-60046] - MUC1 binds RIPK1 to mediate the RIPK1/RIPK3 pathway. (a,b) MUC1 knockdown by MUC1‐siRNA or NC‐siRNA were assayed in 16HBE cells following stimulation with TNF‐ alpha (300 ng/ml). After 24 hr of stimulation, RIPK3 & RIPK1 protein levels were measured by western blot analysis. (c,d) Normal 16HBE cells were treated with TNF‐ alpha for 24 hr, & the interaction between MUC1‐CT & RIPK1/RIPK3 were confirmed by immunoprecipitation. Data are expressed as means ± SD & were analyzed by one‐way analysis of variance, *p < 0.05. NC group (transfected with NC siRNA); MUC1‐siRNA group (transfected with MUC1‐siRNA). 16HBE: human bronchial epithelial; IB: immunoblotting; IP: immunoprecipitation; RIPK1: receptor‐interacting protein kinase‐1; RIPK3: receptor‐interacting protein kinase‐3; NC: negative control; siRNA: small interfering RNA; TNF‐ alpha : tumor necrosis factor‐ alpha Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30666647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-60046_rabbit-polyclonal-muc1-antibody-310202416175235.jpg)
![Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26. WB was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:300. Lane 2: Antibody dilution 1:500. Lane 3: Antibody dilution 1:1000.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-PCK-26-Western-Blot-NB120-6401-img0011.jpg "Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26. WB was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:300. Lane 2: Antibody dilution 1:500. Lane 3: Antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Human pancreas cancer cells. Antibody at 1:250 dilution. Overnight incubation. No permeabilization. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunocytochemistry-Immunofluorescence-NB120-6401-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Staining of FFPE human placenta sections with 1:300 Monoclonal Anti-Cytokeratin, pan Clone: PCK-26, followed by Goat Anti Mouse IgG (Fab)-FITC.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-PCK-26-Immunohistochemistry-Paraffin-NB120-6401-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Detection of EMT & increased motility in NHRI-HN1 cells. (A) Summary of the most enriched pathways associated with tumorigenesis in syngeneic mice by comparing tumorigenic NHRI-HN1 cells with nontumorigenic cells, including M1-2, M2-3 & NHRI-HN2, using GSEA analysis. Blue indicates a negative normalized enrichment score (NES) & orange indicates a positive NES. (B) Morphology of M1-2 & NHRI-HN1 cells via phase-contrast microscopy at 100× magnification. (C) Relative adhesion activity in M1-2 & NHRI-HN1 cells, determined by normalizing the mean OD 490 nm value of NHRI-HN1 cells to that of M1-2 cells. (D) Cells stained with Alexa Fluor 488 phalloidin, anti-pan-CK & anti-EGFR antibodies at 400× magnification. (E) Immunoblot analysis of epithelial (E-cadherin, alpha -catenin & beta -catenin), mesenchymal (N-cadherin & Vimentin) proteins & EMT-related transcription factors, including Twist, Snail & Slug in M1-2 & NHRI-HN1 cells. Protein levels were normalized to an internal control, alpha -tubulin. Ratios were determined by dividing the normalized protein levels in NHRI-HN1 cells by that in M1-2 cells. (F) Representative images (left) & relative data (right) for migration activity of M1-2 & NHRI-HN1 cells. (G) Representative images (left) & relative data (right) for invasion activity of M1-2 & NHRI-HN1 cells at 200× magnification. The relative migration or invasion activity was determined by normalizing the mean number of cells that have migrated or invaded per field of NHRI-HN1 cells to that of M1-2 cells. Error bars represent SE; ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31878324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-6401_mouse-monoclonal-cytokeratin-pan-antibody-pck-26-310202416212383.jpg)
![Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0004.jpg "Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-222] - 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Antibody-Immunocytochemistry-Immunofluorescence-NB300-222-img0002.jpg)
![Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0003.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:3000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0033.jpg "Western Blot: beta-Catenin Antibody [NBP1-32239] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - Paraformaldehyde-fixed A431, using beta- Catenin antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32239-img0017.jpg)
![Chromatin Immunoprecipitation (ChIP): beta-Catenin Antibody [NBP1-32239] - Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ug of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-32239-img0024.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Western blot for beta-catenin in HEK 293 cells treated with LiCl (10mM) for 1 hr. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0008.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0013.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGEbeta Catenin antibody [N1N2-2], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32239-img0032.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse urinary bladder diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0019.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse skin dilution: 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0020.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse colon dilution: 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0021.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0036.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0037.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0038.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0039.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0040.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0041.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0042.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - P-cadherin is co-localized with other junctional proteins at the RPE cell border in mice.Immunofluorescence of mouse RPE flat-mounts. Double staining: P-cadherin (red; A, E, I) & either ZO-1 (green; B), beta -catenin (green; F), or F-actin (green; J), with nuclear stain by DAPI (blue; C, G, K). Merged images (D, H, L) show the co-localization of P-cadherin with ZO-1 (tight junction), beta -catenin (adherens junction), & F-actin (adherens junction) at the cell-cell border. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29338041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-310202415171344.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-31020241617146.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-310202416171420.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-31020241618265.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-31020241618934.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - Oxidative stress-induced dissociation of adherens junctions results in nuclear translocation of beta -catenin & an increase of EMT-related factors in mouse RPE.(A) Immunofluorescence of mouse RPE flat-mounts. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & the localization of beta -catenin (green) & P-cadherin (red) was analyzed along with nuclear stain by DAPI (blue) on Days 0 (a-c), 1 (d-f), 3 (g-i) & 7 (j-l). Double staining: beta -catenin (a, d, g, j), P-cadherin (b, e, h, k), & merged images with DAPI (c, f, i, l). The localization of beta -catenin & P-cadherin at the cell-cell border was significantly disrupted, & instead prominently detected on/in the nucleus on Day 3. (B) Immunofluorescence of mouse retinal sections with a focus on the RPE nuclei. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & the localization of beta -catenin (green) & P-cadherin (red) was analyzed along with nuclear stain by DAPI (blue) on Days 0 (m-o) & 3 (two representative nuclei; p-r & s-u). Double staining: beta -catenin (m, p, s), P-cadherin (n, q, t), & merged images with DAPI (o, r, u). On Day 3, beta -catenin was detected in the nuclei of mouse RPE. (C) Western blot analyses of mouse RPE proteins. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & RPE protein lysates were prepared on Days 0, 1, 3, & 7. The protein levels were analyzed using Western blotting with antibodies against P-cadherin, beta -catenin, SNAI1 (Snail), vimentin, & control beta -actin. The protein levels of beta -catenin & SNAI1 increased similarly on Day 1 following oxidative stress. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29338041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-310202416165641.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242011415.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunohistochemical analysis.</br>Sample: Paraffin-embedded cat liver.</br>beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242075974.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunohistochemical analysis.</br>Sample: Paraffin-embedded cat colon.</br>beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420122025.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. <BR/>Sample: 2 days-post-fertilization zebrafish embryo. <BR/>beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.<BR/>](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420134025.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420104461.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - Immunohistochemical analysis of agarose-embedded zebrafish embryo, using beta-Catenin antibody [N1N2-2], N-term NBP1-32239) at 1:100. dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420115056.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. <BR/>Sample: 1 day-post-fertilization zebrafish embryo. <BR/>beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420564.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded rat tissues.</br>beta-Catenin stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-9102024202461.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2] detects beta-Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. <br>Sample: Paraffin-embedded mouse colon. <br>Green: beta-Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500.<br>Red: alpha Tubulin antibody [GT114] diluted at 1:500. <br>Blue: Hoechst 33342 staining.<br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242001070.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. <BR/>Sample: 2 days-post-fertilization zebrafish embryo. <BR/>beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.<BR/>](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-9102024202198.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunofluorescent analysis.</br>Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242010443.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242075983.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded mouse tissues.</br>beta-Catenin stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242075987.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420131247.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (9 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-1610202419405728.jpg)
![beta-Catenin antibody [N1N2-2] detects beta-Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. <br>Sample: Paraffin-embedded mouse colon. <br>Green: beta-Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500.<br>Red: alpha Tubulin antibody [GT114] diluted at 1:500. <br>Blue: Hoechst 33342 staining.<br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-immunohistochemistry-paraffin-1411202518321620.jpg)
![beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) detects CTNNB1 protein by flow cytometry analysis.<br /> Sample: HeLa cell.<br /> Black: Unlabelled sample was used as a control.<br /> Red: beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:50.<br /> Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-flow-cytometry-1411202518333124.jpg)
![beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein at cell membrane by immunofluorescent analysis.<br>Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: beta-Catenin protein stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.<br>Blue: Hoechst 33342 staining.<br>](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-immunocytochemistry-immunofluorescence-1411202518324119.jpg)
![Flow Cytometry: SSEA-3 Antibody (MC-631) [NB100-1832] - A surface stain was performed on Jurkat cells with SSEA-3 Antibody (MC-631) NB100-1832AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/SSEA-3-Antibody-MC-631-Flow-Cytometry-NB100-1832-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody.](http://images.novusbio.com/fullsize/SSEA-3-Antibody-MC-631-Immunocytochemistry-Immunofluorescence-NB100-1832-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-8D3-Immunocytochemistry-Immunofluorescence-NB100-64979-img0002.jpg "Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.")
![Flow Cytometry: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - RPE conjugated rat anti mouse Ter-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse Ter-119 and FITC conjugated rat anti mouse CD71. All experiments performed on mouse bone marrow. Data acquired on the ZE5Cell Analyzer](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-8D3-Flow-Cytometry-NB100-64979-img0001.jpg)
![Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of Integrin beta 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysateLane 2: mouse marrow tissue lysate](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Western-Blot-NBP2-67416-img0012.jpg "Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of Integrin beta 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysateLane 2: mouse marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunocytochemistry-Immunofluorescence-NBP2-67416-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse lung tissue. IF-stained paraffin section. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0010.jpg)
![Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse bone marrow cell lysate with DMSO (lane 1), chemical agonist #1 (lane 2), chemical agonist #2 (lane 3), chemical agonist #3 (lane 4). WB image submitted by a verfied customer review.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Western-Blot-NBP2-67416-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunocytochemistry-Immunofluorescence-NBP2-67416-img0001.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human liver cancer tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human spleen tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse colon tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0006.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse testis tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-beta-3-CD61-Antibody-SJ19-09-Immunohistochemistry-Paraffin-NBP2-67416-img0007.jpg)
![Western blot analysis of U-87 MG cell lysates with NBP2-67416 at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67416 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67416_-western-blot-639204586673762209.jpg)
![Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-Western-Blot-NBP2-22124-img0006.jpg "Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.")
![Immunocytochemistry/Immunofluorescence: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-22124-img0004.jpg)
![Immunohistochemistry-Paraffin: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-22124-img0005.jpg)
![Flow Cytometry: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of HepG2 cells using ABCG2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-Flow-Cytometry-NBP2-22124-img0003.jpg)
![ELISA: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/ABCG2-Antibody-3G8-ELISA-NBP2-22124-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Mouse Monoclonal ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Human brain microvascular endothelial cells stained for cilia (red) and ABCG2 (green) and nuclear DAPI (blue). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-22124_mouse-monoclonal-abcg2-cd338-antibody-3g8-immunocytochemistry-immunofluorescence-612025101023..jpg)
![Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NB300-137-img0004.jpg "Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Analysis of the widely used rat pheochromocytoma PC12 cell line stained with rabbit polyclonal Periphren antibody, dilution 1:2000 (Green). Hoechst staining of nuclear DNA (Blue). Peripherin, one of the Class III family of intermediate filament subunit proteins, is a major component of the PC12 cell forming a perinuclear cap, with some filaments visible in the cytoplasm.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-137-img0003.jpg)
![Western Blot: Peripherin Antibody [NB300-137] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule.](http://images.novusbio.com/fullsize/p_4356adfdef9d7.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-137 antibody to peripherin (green) and chicken polyclonal antibody to phosphorylated NF-H (red channel). A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain NF-H and not peripherin. The blue channel reveals the localization of DNA.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-137-img0001.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Analysis in human cerebral cortex and liver tissues. Corresponding S100B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: S100B Antibody [NBP1-87102] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-Western-Blot-NBP1-87102-img0010.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0025.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in purkinje cell layer.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0022.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0023.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody [NBP1-87102] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/S100B-Antibody-Immunohistochemistry-Paraffin-NBP1-87102-img0024.jpg)
![Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Simple-Western-NB100-64768-img0002.jpg "Simple Western: His Tag Antibody (AD1.1.10) [NB100-64768] - Image shows a specific band for 6-His Epitope Tag in 0.1 mg/mL of rhEDG6 CHO S Tx lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the Biotin conjugate of NB100-64768. Detection of HIS tagged protein probed with mouse anti histidine tag : Biotin and visualised with Streptavidin : HRP.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0008.jpg)
![Flow Cytometry: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the FITC conjugate of NB100-64768. Staining of Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) human peripheral blood lymphocytes, visualised with FITC conjugated Mouse anti-HIS tag antibody](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Flow-Cytometry-NB100-64768-img0007.jpg)
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Drosophila, 20 ug whole cell lysate](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0012.jpg)
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis using the HRP conjugate of NB100-64768. Detection of His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0013.jpg)
![Western Blot: His Tag Antibody (AD1.1.10) [NB100-64768] - Analysis of 1) 6x-His tagged FLCN protein and 2) 6x-His tagged homer protein.](http://images.novusbio.com/fullsize/His-Tag-Antibody-AD1-1-10-Western-Blot-NB100-64768-img0001.jpg)