| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74571SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61646SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86635-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56649SS | |||
![Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NB110-74571-img0004.jpg "Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.")
![Immunocytochemistry/Immunofluorescence: Striatin Antibody [NB110-74571] - Immunofluorescent staining of NIH3T3 cells ± blocking peptide using NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74571-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88345-img0017.jpg "Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining in human testis and kidney tissues . Corresponding NCAPH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0016.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0014.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney, skin, testis and tonsil using Anti-NCAPH antibody NBP1-88345 (A) shows similar protein distribution across tissues to independent antibody NBP1-88346 (B).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human skin shows nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0013.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human tonsil shows nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0015.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Simple Western lane view shows a specific band for NCAPH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0006.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Electropherogram image(s) of corresponding Simple Western lane view. NCAPH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with antibodies against Astrin and tubulin and CREST antisera and stained with DAPI for DNA. Cropped images are 3x magnified showing lateral kinetochores. Scale bars represent 5 um (insets, 2 um). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0960982213007653), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74638-img0003.jpg "Immunocytochemistry/Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with antibodies against Astrin and tubulin and CREST antisera and stained with DAPI for DNA. Cropped images are 3x magnified showing lateral kinetochores. Scale bars represent 5 um (insets, 2 um). Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0960982213007653), licensed under a CC-BY license.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of Immunoprecipitants. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74638 used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74639, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Astrin-Antibody-Immunoprecipitation-NB100-74638-img0002.jpg)
![Immunohistochemistry: Astrin Antibody [NB100-74638] - SKA3 localisation is not abrogated due to the lack of Astrin.(A) Representative deconvolved images show SKA3 enrichment at kinetochores of HeLa cells treated with Control or Astrin siRNA. Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against Astrin & SKA3 & CREST antiserum (to mark centromeres) & stained with DAPI for DNA. Cropped images show Astrin or SKA3 enrichment at kinetochores. (B) Representative images show SKA3 enrichment at kinetochores of HeLa cells treated with Astrin siRNA before transfection with eukaryotic protein expression vectors encoding YFP-Astrin (WT, 4A or ∆70, as indicated). Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against GFP (for detecting YFP-Astrin) & SKA3 & CREST antiserum & co-stained with DAPI for DNA. Cropped images show YFP-Astrin or SKA3 enrichment at kinetochores. Scale as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31808746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74638_rabbit-polyclonal-astrin-antibody-31020241612667.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NB100-373] - SMC2 in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijcb/2017/8607532/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg)
![Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-288-img0018.jpg "Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-NB100-288-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for IP at 3 ug per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody NB100-639. For blotting immunoprecipitated MCM2, NB100-288 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunoprecipitation-NB100-288-img0019.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0025.jpg "Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500")
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PCNA protein stained by PCNA antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0026.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500.](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0020.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug NIH-3T3 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500](http://images.novusbio.com/fullsize/PCNA-Antibody-Western-Blot-NBP1-32075-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32075-img0014.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse testis. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0013.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human colon. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0016.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human cervical carcinoma. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunohistochemistry-Paraffin-NBP1-32075-img0017.jpg)
![Immunoprecipitation: PCNA Antibody [NBP1-32075] - Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 ug of PCBP2 antibody. Western blot analysis was performed using PCBP2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PCNA-Antibody-Immunoprecipitation-NBP1-32075-img0023.jpg)
![Western Blot: PCNA Antibody [NBP1-32075] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (NBP1-32075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32075_rabbit-polyclonal-pcna-antibody-910202420131282.jpg)
![Western Blot: CDT2 Antibody [NB100-40840] - Whole cell lysate (15 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/CDT2-Antibody-Western-Blot-NB100-40840-img0009.jpg "Western Blot: CDT2 Antibody [NB100-40840] - Whole cell lysate (15 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CDT2-Antibody-Immunohistochemistry-Paraffin-NB100-40840-img0011.jpg)
![Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CDT2-Antibody-Immunohistochemistry-Paraffin-NB100-40840-img0010.jpg)
![Immunoprecipitation: CDT2 Antibody [NB100-40840] - Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody NB100-40840 (lot NB100-40840-3) used for IP at 6 ug per reaction. DTL/CDT2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-40840-2). For blotting immunoprecipitated DTL/CDT2, NB100-40840 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/CDT2-Antibody-Immunoprecipitation-NB100-40840-img0008.jpg)
![Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Western-Blot-NB110-61646-img0012.jpg "Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.")
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86635-img0008.jpg "Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NBP1-86635] - Staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SMC4 Antibody [NBP1-86635] - Staining of human kidney shows strong cytoplasmic positivity with granular pattern in tubule cells.](http://images.novusbio.com/fullsize/SMC4-Antibody-Immunohistochemistry-Paraffin-NBP1-86635-img0002.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-86635_rabbit-polyclonal-smc4-antibody-30420251371429.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68209-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Expression pattern of HCF1, MLL, and Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Knockdown-Validated-NB100-68209-img0011.jpg)
![Immunoprecipitation: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Interaction between MLL and HCF1was examined by immunoprecipitation analysis using anti-MLL and anti-HCF1 antibodies followed by Western blotting with anti-HCF1 and anti-MLL antibodies. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Immunoprecipitation-NB100-68209-img0012.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. E. Expression patterns of TET1, OGT, HCF1, & MLL in NAC pre-treated SNUC5/5-FUR cells determined by WB analysis. *Significantly different from SNUC5 cells (p<0.05), & #significantly different from SNUC5/5-FUR cells (p<0.05). Also, SNUC5 cells pre-treated with 500 μM of NAC & incubated for an additional 1 h at 37°. Cells then treated with 140 μM 5-FU for 48 h. 10 μM of DHR123 was added to plate & incubated for an additional 30 min at 37°C. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415171343.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. J. Expression of TET1 & interaction proteins detected by WB analysis. *Significantly different from SNUC5 cells (p<0.05), & #significantly different from 5-FU-treated SNUC5 cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415345312.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - 5-FU-produced ROS involved in histone methyltransferase-mediated Nrf2 expression. F. The intracellular ROS level was detected by flow cytometry. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-31020241534395.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415532061.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Relation of OGT-TET1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. Interaction between OGT & TET1 was examined by immunoprecipitation analyses using anti-OGT & anti-TET1 antibodies followed by Western blotting with anti-TET1 & anti-OGT antibodies. B. Interaction between OGT & TET1 was assessed by using PLA. Each green spot represents for a single interaction (OGT & TET1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). C. The O-GlcNAcylated TET1 or O-GlcNAcylated OGT was examined by immunoprecipitation analyses using anti-O-GlcNAc antibody followed by Western blotting with anti-TET1 & anti-OGT antibodies. The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA or siTET1 RNA for 24 h. D. Expression pattern of OGT & TET1 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of TET1, OGT, HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415523922.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Involvement of OGT in histone methylase MLL/COMPASS-like complex-mediated Nrf2 expression in SNUC5/5-FUR cellsA. OGT & O-GlcNAc expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between HCF1 & OGT was examined by immunoprecipitation analysis using anti-HCF1 & anti-OGT antibodies followed by Western blotting with anti-HCF1 & anti-OGT antibodies. C. Interaction between HCF1 & OGT was assessed by using PLA. Each green spot represents for a single interaction (HCF1 & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). D. Interaction between MLL & OGT was examined by immunoprecipitation analysis using anti-MLL & anti-OGT antibodies followed by Western blotting with anti-OGT & anti-MLL antibodies. E. Interaction between MLL & OGT was assessed by using PLA. Each green spot represents for a single interaction (MLL & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA for 24 h. F. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). G. Expression pattern of OGT, MLL, HFC1, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415532084.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Involvement of OGT in histone methylase MLL/COMPASS-like complex-mediated Nrf2 expression in SNUC5/5-FUR cellsA. OGT & O-GlcNAc expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between HCF1 & OGT was examined by immunoprecipitation analysis using anti-HCF1 & anti-OGT antibodies followed by Western blotting with anti-HCF1 & anti-OGT antibodies. C. Interaction between HCF1 & OGT was assessed by using PLA. Each green spot represents for a single interaction (HCF1 & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). D. Interaction between MLL & OGT was examined by immunoprecipitation analysis using anti-MLL & anti-OGT antibodies followed by Western blotting with anti-OGT & anti-MLL antibodies. E. Interaction between MLL & OGT was assessed by using PLA. Each green spot represents for a single interaction (MLL & OGT) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siOGT RNA for 24 h. F. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). G. Expression pattern of OGT, MLL, HFC1, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415525241.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415523923.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Effect of MLL-HCF1 interaction on histone methylation & Nrf2 expression in SNUC5/5-FUR cellsA. HCF1 expression was determined by Western blotting. *Significantly different from SNUC5 cells (p<0.05). B. Interaction between MLL & HCF1was examined by immunoprecipitation analysis using anti-MLL & anti-HCF1 antibodies followed by Western blotting with anti-HCF1 & anti-MLL antibodies. *Significantly different from SNUC5 cells (p<0.05). C. Interaction between MLL & HCF1was assessed by PLA. Each green spot represents for a single interaction (MLL & HCF1) & DNA was stained with DAPI. *Significantly different from SNUC5 cells (p<0.05). The cells were transfected with non-targeting siRNA (siControl) or siHCF1 RNA for 24 h. D. Expression pattern of MLL mRNA in SNUC5/5-FUR cells was determined by RT-PCR analysis. *Significantly different from siControl-transfected cells (p<0.05). E. Expression pattern of HCF1, MLL, & Nrf2 in SNUC5/5-FUR cells was determined by Western blot analysis. *Significantly different from siControl-transfected cells (p<0.05). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.9745), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68209_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415533831.jpg)
![Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Western-Blot-NB100-56649-img0003.jpg "Western Blot: Lamin A + C Antibody [NB100-56649] - Analysis of human Lamin A + C in 15 ug of HeLa cell lysate using NB100-56649 at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NB100-56649] - Lamin A/C antibody was tested in A431 cells at 1:100 against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56649-img0005.jpg)
![Flow (Intracellular): Lamin A + C Antibody [NB100-56649] - Intracellular staining of HeLa cells (1 x 10^6 cells/mL) with Lamin A+C antibody (orange) stained at 1:1000. Detected with a GtxRb DyLight 488 secondary. Shown with the secondary only control (blue).](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Flow-Intracellular-NB100-56649-img0004.jpg)
![Western Blot: MCM7 Antibody (6C2) [H00004176-M01] - MCM7 monoclonal antibody (M01), clone 6C2 Analysis of MCM7 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Western-Blot-H00004176-M01-img0012.jpg "Western Blot: MCM7 Antibody (6C2) [H00004176-M01] - MCM7 monoclonal antibody (M01), clone 6C2 Analysis of MCM7 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Immunocytochemistry-Immunofluorescence-H00004176-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Immunohistochemistry-Paraffin-H00004176-M01-img0010.jpg)
![Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDK7 and MCM7. HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Proximity-Ligation-Assay-H00004176-M01-img0005.jpg)
![Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-Proximity-Ligation-Assay-H00004176-M01-img0006.jpg)
![ELISA: MCM7 Antibody (6C2) [H00004176-M01] - Detection limit for recombinant GST tagged MCM7 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM7-Antibody-6C2-ELISA-H00004176-M01-img0011.jpg)