| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88861-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80819 | |||
| BSA Free | NB100-507SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50599-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22151-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-178SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-96140SS | |||
| Azide and BSA Free | NBP2-80599 | |||
![Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/USP22-Antibody-Western-Blot-NBP1-49644-img0001.jpg "Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.")
![Western Blot: USP22 Antibody [NBP1-49644] - analysis of USP22 in HeLa whole cell lysate using anti-USP22 antibody. The primary antibody was used at a dilution of 1:500 and incubated overnight at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/USP22-Antibody-Western-Blot-NBP1-49644-img0002.jpg)
![Simple Western: USP22 Antibody [NBP1-49644] - Simple Western lane view shows a specific band for USP22 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/USP22-Antibody-Simple-Western-NBP1-49644-img0003.jpg)
![Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Western-Blot-NBP1-88861-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Simple Western lane view shows a specific band for PARD3/Par3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Simple-Western-NBP1-88861-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line U-2 OS shows localization to cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining PARD3 in mouse trophoblast stem cells. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0012.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human colon shows distinct luminal membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. In wild-type myotubes PAR3 remains cytoplasmic. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28923978/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0023.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human skin shows weak to moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cell line 8505C cells (anaplastic thyroid cancer cell line). Image from verified customer review.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88861-img0013.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0019.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human cerebellum shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0020.jpg)
![Immunohistochemistry-Paraffin: PARD3/Par3 Antibody [NBP1-88861] - Staining of human fallopian tube shows weak to moderate positivity in luminal membrane in glandular cells.](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Immunohistochemistry-Paraffin-NBP1-88861-img0021.jpg)
![Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Electropherogram image(s) of corresponding Simple Western lane view. PARD3/Par3 antibody was used at 1:100 dilution on RT-4 lysate(s).](http://images.novusbio.com/fullsize/PARD3-Par3-Antibody-Simple-Western-NBP1-88861-img0015.jpg)
![Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean±s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88861_rabbit-polyclonal-pard3-par3-antibody-310202416113535.jpg)
![Immunocytochemistry/ Immunofluorescence: PARD3/Par3 Antibody [NBP1-88861] - MCAM is required to establish cell autonomous polarity. (A) In elongating myotubes (10T1/2 cells treated with testosterone for 7 days) VANGL2 is localized asymmetrically at the tip of the cell. (B) The VANGL2 enriched tip of the cell is marked by MSN. (C) In MCAM knockout C164 cells myotube elongation fails, MSN labels the whole plasma membrane & VANGL2 is spread across the cytoplasm. (D) Highly polarized localization of MCAM & SCRIB at the distal end of growing wild-type myotube. Separate channels of the boxed area are shown on the right. (E) In MCAM knockout cells SCRIB levels remain low & it is spread evenly in the cell. (F) In wild-type myotubes PAR3 remains cytoplasmic, whereas (G) in MCAM knockout C164 cells it can be detected at the cell cortex. (H) RT-qPCR demonstrates reduced expression of Scrib in MCAM mutant cell lines. Cells were treated for 7 days with BMP2 or testosterone (n=3; **P<0.01; ***P<0.001; two-tailed t-test; mean±s.e.m.). (I) Deletion of MCAM endocytosis motif leads to similar polarity defects as complete MCAM elimination. VANGL2 is evenly spread in U125 cells & PAR3 accumulates in cell cortex. (J) In chondrogenic differentiation VANGL2 was observed asymmetrically in limited number of cells. In MCAM mutant cell lines (C149, C164, U125) VANGL2 accumulated around the nucleus. (K) Initiation of myogenic (4-day culture with testosterone) & chondrogenic differentiation (4-day culture with BMP2) led to downregulation of ERK1/2 phosphorylation (p-ERK1/2). Instead in MCAM mutant cell lines ERK1/2 phosphorylation increased. Scale bars: 25 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/bio/article/doi/10.1242/bio.027771/256759/MCAM-contributes-to-the-establishment-of-cell), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88861_rabbit-polyclonal-pard3-par3-antibody-310202416113539.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW128) at 5 ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Immunocytochemistry-Immunofluorescence-NB100-507-img0005.jpg "Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW128) at 5 ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.")
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Detection of p300 in a HeLa nuclear extract using NB100-507 (1:250). ECL: 15 sec exposure.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0003.jpg)
![Flow (Intracellular): KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on THP-1 cells with KAT3B/p300 (RW128) antibody NB100-507 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Flow-Intracellular-NB100-507-img0004.jpg)
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Subcellular fractionation and localization of Src and p300 in Panc-1 cells. Cell lysates of equal total protein or Panc-1 subcellular fractions were subjected to immunoblotting analysis for Src, pSrc, p300, tubulin (cytoskeletal proteins), GAPDH (soluble cytoplasmic proteins), calnexin (endoplasmic reticulum), grim19 (mitochondria), histone H3 (chromatin), and lamin A/C (nuclear lamina). Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW128) NB100-507 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Immunocytochemistry-Immunofluorescence-NB100-507-img0009.jpg)
![Flow Cytometry: KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on Raw264.7 cells with KAT3B/p300 Antibody (RW128) NB100-507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Flow-Cytometry-NB100-507-img0010.jpg)
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Src and p300 co-association in Panc-1 cells. Immunoblotting analysis of Src or p300 immunecomplexes from Panc-1 nuclear isolates probing for Src or p300, or IgG control. Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0006.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/EGFR-Antibody-DH8-3-EGFRvIII-Flow-Cytometry-NBP2-50599-img0002.jpg "Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.")
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/EGFR-Antibody-DH8-3-EGFRvIII-Flow-Cytometry-NBP2-50599-img0001.jpg)
![Flow Cytometry: Mouse Monoclonal EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - Analysis of glioblastoma cell lines using EGFR Antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-50599_mouse-monoclonal-egfr-antibody-dh8-3-egfrviii-mutant-flow-cytometry-145202414346..png)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Expression of HDACs in murine optic nerve. Western blot analysis of HDAC proteins in E16, P5 and P30 murine optic nerve was performed to check the specificity of the antibodies used for the study. Blots were normalized to beta-TUBULIN. Densitometric graphs for each HDAC panel show quantification of protein levels expressed as fold-change from E16. p < 0.05 was considered statistically significant and represented by a single *, whereas p < 0.005 is represented by **. Bars show mean +/- SEM of three samples per timepoint. Note that the apparent increase in band intensities for some P5 samples reflect increased protein loading in those lanes. Image collected and cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-14-30), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NBP2-22151-img0006.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Expression of HDACs in murine optic nerve. Western blot analysis of HDAC proteins in E16, P5 and P30 murine optic nerve was performed to check the specificity of the antibodies used for the study. Blots were normalized to beta-TUBULIN. Densitometric graphs for each HDAC panel show quantification of protein levels expressed as fold-change from E16. p < 0.05 was considered statistically significant and represented by a single *, whereas p < 0.005 is represented by **. Bars show mean +/- SEM of three samples per timepoint. Note that the apparent increase in band intensities for some P5 samples reflect increased protein loading in those lanes. Image collected and cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-14-30), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 antibody was tested in HeLa cells at a 1:100 dilution against DyLight 488 (Green) using a 40X objective. Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22151-img0005.jpg)
![Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 localization pattern in developing murine optic nerve. Optic nerve sections at P30 were triple-labeled with HDAC4 (red). Double-label of SOX2 and HDAC4. Composite image in M show z stacks of optic nerve at high magnification; horizontal yellow lines correspond the x axis plane and vertical yellow lines corresponds to the y axis. The x, z axis is shown at the bottom of each panel, while the y, z axis is shown to the right of the panel. Abbreviations: R; retina, ON; optic nerve. Scale bars = 50 um. Image collected and cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-14-30), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Immunohistochemistry-NBP2-22151-img0007.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Analysis of HDAC4 in NIH/3T3 lysate.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NBP2-22151-img0003.jpg)
![Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Analysis of HDAC4 in mouse pancreas.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Immunohistochemistry-NBP2-22151-img0002.jpg)
![Simple Western: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Image shows a specific band for HDAC4 in 0.5 mg/mL of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Simple-Western-NBP2-22151-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)](http://images.novusbio.com/fullsize/ZCCHC11-TUT4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-783-img0003.jpg "Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)")
![Immunohistochemistry-Paraffin: ZCCHC11/TUT4 Antibody [NB100-783] - Staining of paraffin embedded Human Lymph Node. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/ZCCHC11-TUT4-Antibody-Immunohistochemistry-Paraffin-NB100-783-img0002.jpg)
![Western Blot: FOX2 Antibody [NB110-40588] - Whole cell lysate (10 ug) from NIH 3T3, TCMK-1, CT26, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FOX2-Antibody-Western-Blot-NB110-40588-img0014.jpg "Western Blot: FOX2 Antibody [NB110-40588] - Whole cell lysate (10 ug) from NIH 3T3, TCMK-1, CT26, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: FOX2 Antibody [NB110-40588] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RBM9 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FOX2-Antibody-Immunohistochemistry-Paraffin-NB110-40588-img0016.jpg)
![Western Blot: FOX2 Antibody [NB110-40588] - Whole cell lysate (10 ug) from HEK293T, HeLa, RKO, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FOX2-Antibody-Western-Blot-NB110-40588-img0013.jpg)
![Immunohistochemistry-Paraffin: FOX2 Antibody [NB110-40588] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- usedat a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FOX2-Antibody-Immunohistochemistry-Paraffin-NB110-40588-img0015.jpg)
![Immunoprecipitation: FOX2 Antibody [NB110-40588] - Detection of human RBM9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody NB110-40588 (lot NB110-40588-2) used for IP at 6 ug/mg lysate. RBM9 was also immunoprecipitated by a previous lot (lot NB110-40588-1) of this antibody. For blotting immunoprecipitated RBM9, NB110-40588 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FOX2-Antibody-Immunoprecipitation-NB110-40588-img0012.jpg)
![Western Blot: mtTFA Antibody [NBP2-19437] - Mitochondrial-related protein expression in LA tissue. Representative Western blot analysis of the protein expression in TFAM. Data is mean+/-SD, relative protein level was normalized to beta-actin. *Compared with the control group PSample: Paraffin-embedded human breast carcinoma.mtTFA stained by mtTFA antibody (NBP2-19437) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19437_rabbit-polyclonal-mttfa-antibody-immunohistochemistry-paraffin-2611202518431923.jpg "Western Blot: mtTFA Antibody [NBP2-19437] - Mitochondrial-related protein expression in LA tissue. Representative Western blot analysis of the protein expression in TFAM. Data is mean+/-SD, relative protein level was normalized to beta-actin. *Compared with the control group PSample: Paraffin-embedded human breast carcinoma.mtTFA stained by mtTFA antibody (NBP2-19437) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![mtTFA antibody detects mtTFA protein at mito by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mtTFA stained by mtTFA antibody (NBP2-19437) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19437_rabbit-polyclonal-mttfa-antibody-immunocytochemistry-immunofluorescence-261120251831541.jpg)
![Western Blot: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - UBE2C monoclonal antibody (M01), clone 9D3 Analysis of UBE2C expression in HeLa.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-9D3-Western-Blot-H00011065-M01-img0014.jpg "Western Blot: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - UBE2C monoclonal antibody (M01), clone 9D3 Analysis of UBE2C expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - Analysis of monoclonal antibody to UBE2C on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-9D3-Immunocytochemistry-Immunofluorescence-H00011065-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - Analysis of monoclonal antibody to UBE2C on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-9D3-Immunohistochemistry-Paraffin-H00011065-M01-img0012.jpg)
![Western Blot: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - Analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody (M01), clone 9D3.Lane 1: UBE2C transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-9D3-Western-Blot-H00011065-M01-img0015.jpg)
![ELISA: UbcH10/UBE2C Antibody (9D3) [H00011065-M01] - Detection limit for recombinant GST tagged UBE2C is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/UbcH10-UBE2C-Antibody-9D3-ELISA-H00011065-M01-img0016.jpg)
![Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NB500-178-img0005.jpg "Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.")
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Numb Antibody (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0007.jpg)
![Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Numb-Antibody-Simple-Western-NB500-178-img0006.jpg)
![Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunohistochemistry-NB100-563-img0006.jpg "Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MAD2L1-Antibody-Immunoprecipitation-NB100-563-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0008.jpg "Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-96140 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0009.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0003.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on NIH3T3 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0012.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Western-Blot-NBP1-96140-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0005.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0007.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U937 cells with BMI-1 Antibody (LLBmi1-1) NBP1-96140 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/BMI-1-Antibody-LLBmi1-1-Flow-Cytometry-NBP1-96140-img0011.jpg)