| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83940-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47254SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89388-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27406SS | |||
| Azide and BSA Free | NBP2-80834 | |||
![Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Hela cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the -39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.](http://images.novusbio.com/fullsize/p38-[p-Thr180--p-Tyr182]-Antibody-Western-Blot-NB500-138-img0005.jpg "Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Hela cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the -39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.")
![Immunohistochemistry-Paraffin: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red.](http://images.novusbio.com/fullsize/p38-[p-Thr180-p-Tyr182]-Antibody-Immunohistochemistry-Paraffin-NB500-138-img0002.jpg)
![Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182.](http://images.novusbio.com/fullsize/p38-[p-Thr180-p-Tyr182]-Antibody-Western-Blot-NB500-138-img0001.jpg)
![Western Blot: p38 alpha [p Thr180, p Tyr182] Antibody [NB500-138] - RIP2 is not essential for MAPK pathways in the early ontogenesis. (A) Immunoblot analysis of phospho-p44/42 MAPK, p44/42 MAPK, & p38 MAPK in larvae homogenate from WT & NOD1-1IS−/− zebrafish at 7 days post-fertilization (dpf). (B) Immunoblot analysis of phospho-p44/42 MAPK, p44/42 MAPK, & p38 MAPK in larvae homogenate from WT & RIP2−/− zebrafish at 7 dpf. (C) Immunoblot analysis of Atg5, p62, & LC3b in larvae homogenate from WT & NOD1-1IS−/− zebrafish at 7 dpf. (D) Immunoblot analysis of Atg5, p62, & LC3b in larvae homogenate from WT & RIP2−/− zebrafish at 7 dpf. Western blotting results were quantified using Quantity One software. Data represent the average of two independent experiments. *p < 0.05, **p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29692779), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-138_rabbit-polyclonal-p38-alpha-p-thr180-p-tyr182-antibody-31020241534371.jpg)
![Immunocytochemistry/ Immunofluorescence: CD81 Antibody (M38) [NBP1-44861] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP1-44861) at 15 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Knockout-Validated-NBP1-44861-img0006.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: CD81 Antibody (M38) [NBP1-44861] - CD81 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in CD81 knockout (KO) HeLa cell line using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # NBP1-44861) at 15 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane.")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0010.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Surface staining of CD81 in human peripheral blood with anti-CD81 (M38) APC-CyTM7.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0001.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) APC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0002.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Human peripheral blood with anti-CD81 (M38) FITC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0003.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Flow Cytometry: CD81 Antibody (M38) [PE] [NBP1-44861PE] - A cell surface stain was performed on THP-1 cells with CD81 antibody (M38) NBP1-44861 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0004.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) PE.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0005.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) PE.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0007.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Staining of CD81 in human peripheral blood with anti-CD81 (M38) biotin, streptavidin-APC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0008.jpg)
![Flow Cytometry: CD81 Antibody (M38) [NBP1-44861] - Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD81-Antibody-M38-Flow-Cytometry-NBP1-44861-img0009.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining in human skin and pancreas tissues. Corresponding KLF4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining in human skin and pancreas tissues. Corresponding KLF4 RNA-seq data are presented for the same tissues.")
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody [NBP1-83940] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83940-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: KLF4 Antibody [NBP1-83940] - Human breast cancer cell MCF-7 was treated with carboplatin, alone or in combination with Stat3 inhibitor, for 72 hours, and the expression of Klf4 was detected by Western blot. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP1-83940-img0022.jpg)
![Western Blot: KLF4 Antibody [NBP1-83940] - Analysis in control (vector only transfected HEK293T lysate) and KLF4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP1-83940-img0023.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: KLF4 Antibody [NBP1-83940] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP1-83940-img0024.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0017.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0018.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human skin shows moderate to strong nuclear positivity in keratinocytes.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0019.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0020.jpg)
![Western Blot: KLF4 Antibody [NBP1-83940] - HIFs are required for hypoxia-induced expression of pluripotency factorsA-C. Breast cancer cell lines were exposed to 20% or 1% O2 for 24 h & NANOG (A), KLF4 (B), & SOX2 (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, & normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O2 (mean ± SEM; n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. same cell line at 20% O2 by Student's t test. D & E. HCC-1954 (D) & MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1 alpha (sh1 alpha ) or HIF-2 alpha (sh2 alpha ), or vectors encoding shRNAs targeting both HIF-1 alpha & HIF-2 alpha (DKD), were exposed to 20% or 1% O2 for 24 h & RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O2 (mean ± SEM; n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. NTC at 20% O2; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. NTC at 1% O2 by ANOVA. F. ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O2 for 24 h & SOX2 mRNA was measured (mean ± SEM; n = 3). *P < 0.05, **P < 0.01 vs. NTC at 20% O2; ###P < 0.001 vs. NTC at 1% O2 by ANOVA. G & H. NTC & DKD subclones of HCC-1954 (G) & MCF-7 (H) were exposed to 20% or 1% O2 for 48 h, whole cell lysates were prepared, & immunoblot assays were performed to analyze HIF-1 alpha , HIF-2 alpha , NANOG & KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O2 for 48 h, & HIF-1 alpha , NANOG & SOX2 immunoblot assays were performed. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-83940_rabbit-polyclonal-klf4-antibody-310202416121929.jpg)
![Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/USP22-Antibody-Western-Blot-NBP1-49644-img0001.jpg "Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.")
![Western Blot: USP22 Antibody [NBP1-49644] - analysis of USP22 in HeLa whole cell lysate using anti-USP22 antibody. The primary antibody was used at a dilution of 1:500 and incubated overnight at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/USP22-Antibody-Western-Blot-NBP1-49644-img0002.jpg)
![Simple Western: USP22 Antibody [NBP1-49644] - Simple Western lane view shows a specific band for USP22 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/USP22-Antibody-Simple-Western-NBP1-49644-img0003.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0034.jpg "<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunohistochemistry-Paraffin-NB100-464-img0029.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Western-Blot-NB100-464-img0032.jpg)
![Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 (lot 2) used for IP at 6 ug/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated Chk1, NB100-464 (lot 2) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Chk1-Antibody-Immunoprecipitation-NB100-464-img0033.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells.(G) WB for indicated proteins in clones indicated on the top. Darker exposure of ORC2 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415371972.jpg)
![Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC1 in HCT116 p53-/- cells.(A) Strategy for insertion of a blasticidin gene & poly A site after first methionine of ORC1 in the second exon. (B) PCR on genomic DNA of indicated clones. WT: HCT116 p53-/- & ORC1+/+. ORC1 Knockout clones, B14, B48 & BP32 have an insert on both alleles of ORC1 as indicated by the absence of 0.6 kb PCR product. (C) Western blot for indicated proteins in clones indicated on the top. Darker exposure of the ORC1 blots is shown at the bottom. Ponceau S stains all proteins on the blot & also indicates equal loading of lanes. (D) Immunoblot of soluble & chromatin-associated proteins in the clones indicated at the top. For each panel, all the lanes are from the same blot & exposure. (E) Input cell lysate & immunoprecipitates of ORC1 immunoblotted for ORC1. Darker exposure of the top blots is shown in the middle. Tubulin in the cell lysate or the IgG band in the immunoprecipitate serves as loading control.DOI://dx.doi.org/10.7554/eLife.19084.005 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/19084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-464_rabbit-polyclonal-chk1-antibody-310202415525253.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0015.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Staining (6ug/ml) of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NB100-2833-img0016.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NB100-2833] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NB100-2833-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia and neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), and neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) and 4 (NeuN) experimental mice. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0204941), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0014.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NB100-2833-img0017.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia & neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), & neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) & 4 (NeuN) experimental mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-3102024153430.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-31020241535694.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415363735.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - IL-1 beta synthesis, & microglial activation modulation in PDD005-treated aged mice. (A) Images showing IL-1 beta (green fluorescence), iba-1 (red fluorescence) expression. Yellow = Overlay; Blue = nuclei; SGZ region of mouse brain. 40X magnification, scale bar = 100 μm. (B) Graphs representing the average relative fluorescence of Iba-1 in SGZ. Significant differences determined by using one-way ANOVA with Tukey’s test. *P > Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26536027), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-31020241553740.jpg)
![Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)](http://images.novusbio.com/fullsize/ZCCHC11-TUT4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-783-img0003.jpg "Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)")
![Immunohistochemistry-Paraffin: ZCCHC11/TUT4 Antibody [NB100-783] - Staining of paraffin embedded Human Lymph Node. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/ZCCHC11-TUT4-Antibody-Immunohistochemistry-Paraffin-NB100-783-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16908-img0041.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: AIF-1:Iba1 Antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0032.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0035.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0036.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0014.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-16908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16908_rabbit-polyclonal-aif-1-iba1-antibody-910202419593573.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0012.jpg "Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. *=vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0011.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0010.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0007.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. The different cell types in the cellular epidermis as well as the blood vessels in the dermal layer showed positivity for ERR alpha, and the staining was found to be more intense in the nuclei compared to cytoplasm. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0009.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Analysis in human testis and pancreas tissues using HPA002866 antibody. Corresponding SSBP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Analysis in human testis and pancreas tissues using HPA002866 antibody. Corresponding SSBP1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: SSBP1 Antibody [NBP1-80720] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-SSBP1 antibody. Remaining relative intensity is presented. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Western-Blot-NBP1-80720-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: SSBP1 Antibody [NBP1-80720] - Mitochondrial components segregate into MVBs and are secreted in exosomes. Confocal co-localization analysis in HEK293 cells treated with FCCP (4 h) and stained with SSBP1 (red), EEA1 and CD63 (green). Bar, 10 um. Graph shows means for Mander's co-localization coefficient for SSBP1 and EEA1 or CD63 (n = 3). t-test: ***P-value < 0.0001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05077-9), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80720-img0023.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0020.jpg)
![Western Blot: SSBP1 Antibody [NBP1-80720] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Western-Blot-NBP1-80720-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SSBP1 Antibody [NBP1-80720] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80720-img0010.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human cerebral cortex shows granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0017.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human duodenum shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0018.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human pancreas shows weak granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: S100A6 Antibody [NBP1-89388] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89388-img0018.jpg "Immunocytochemistry/Immunofluorescence: S100A6 Antibody [NBP1-89388] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0022.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0019.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules and in glomeruli.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0020.jpg)
![Immunohistochemistry-Paraffin: S100A6 Antibody [NBP1-89388] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/S100A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89388-img0021.jpg)
![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Western-Blot-NBP2-27406-img0009.jpg "Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - LIF expression in human primary T-cells and cancer cell lines: Jurkat and K562. Image from verified customer review.")
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0008.jpg)
![Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/mL concentration of primary antibody, Goat anti-rat IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Western-Blot-NBP2-27406-img0001.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity of LIF was observed specifically in the epithelial cells of prostate alveolar glands, whereas the surrounding fibromuscular stroma cells did not develop any staining.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0002.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0003.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0004.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0006.jpg)
![Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - Tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immune positivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.](http://images.novusbio.com/fullsize/LIF-Antibody-39N7D10-Immunohistochemistry-Paraffin-NBP2-27406-img0007.jpg)
