HAF005). A specific band was detected for Desmocollin-1 at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for Desmocollin-1 at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Desmocollin-1 at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_lightbox"> MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_lightbox"> MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to hair root sheath. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to hair root sheath. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF005). Specific bands were detected for Desmocollin-3 at approximately 95-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). Specific bands were detected for Desmocollin-3 at approximately 95-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse skin tissue and mouse embryo (15 d.p.c.) tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Desmocollin-3 Monoclonal Antibody (Catalog # MAB7265) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Desmocollin-3 at approximately 95-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm in epidermis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm in epidermis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Desmocollin-2 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Desmocollin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7490) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF016). Specific bands were detected for Desmocollin-3 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). Specific bands were detected for Desmocollin-3 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse embryo (15 d.p.c.) tissue and mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Desmocollin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Desmocollin-3 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis of the developing limb bud. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis of the developing limb bud. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />