Desmocollin-2 Antibody

Desmocollin-2 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Desmocollin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7490) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Desmocollin-2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-2
  Arg136-Pro694
  Accession # P55292
• Specificity: Detects mouse Desmocollin-2 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Desmocollin-2 is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Desmocollin-1 and rmDesmocollin-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DSC2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmocollin-2 Antibody

• ARVD11
• Cadherin family member 2
• CDHF2
• CDHF2DGII/III
• desmocollin 2
• Desmocollin2
• Desmocollin-2
• Desmocollin-3
• Desmosomal glycoprotein II
• desmosomal glycoprotein II/III
• Desmosomal glycoprotein III
• DG2
• DSC2
• DSC3DKFZp686I11137

Background

DSC-2 (Desmocollin [Greek for "glue-that-binds"]-2; also epithelial type 2 desmocollin) is a 100-120 kDa member of the Ca⁺⁺-dependent cadherin family of adhesion molecules. It is found on the surface of both simple and stratified epithelium that form desmosomes. DSC-2 expression is induced by DSG-2 where it serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. Homodimeric DSC-2 forms in-trans, and DSC-2 may also form heterotypic interactions with DSG-2. A DSC-2:DSG-2 cis-interaction is suggested to block DSG-2 activation of Akt, an action that results in beta -catenin mediated cell proliferation. Mature mouse DSC-2 is a 767 amino acid (aa) type I transmembrane glycoprotein (aa 136-902). The mature molecule contains a 559 aa extracellular region (aa 136-694) with five cadherin domains (aa 136-694) and a 187 aa cytoplasmic tail (aa 716-902). There is one 100-110 kDa splice variant that shows an 11 aa substitution for aa 838-902. Over aa 136-694, mouse DSC-2 shares 87% and 79% aa sequence identity with rat and human DSC-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Desmocollin-2 Antibody (AF7490)(3)

Publications using AF7490

• M Vallverdú-, D Carreras, GJ Pérez, O Campuzano, R Brugada, M Alcalde Alterations in Calcium Handling Are a Common Feature in an Arrhythmogenic Cardiomyopathy Cell Model Triggered by Desmosome Genes Loss International Journal of Molecular Sciences, 2023-01-20;24(3):. 2023-01-20 [PMID: 36768439] (Western Blot, Mouse)
• Sven Flemming, Anny-Claude Luissint, Dennis H. M. Kusters, Arturo Raya-Sandino, Shuling Fan, Dennis W. Zhou et al. Desmocollin-2 promotes intestinal mucosal repair by controlling integrin-dependent cell adhesion and migration Molecular Biology of the Cell 3/15/2020 [PMID: 31967937]
• Sven Flemming, Anny-Claude Luissint, Dennis H. M. Kusters, Arturo Raya-Sandino, Shuling Fan, Dennis W. Zhou et al. Desmocollin-2 promotes intestinal mucosal repair by controlling integrin-dependent cell adhesion and migration Molecular Biology of the Cell 2020-03-15 [PMID: 31967937] (Western Blot, Human, Mouse)

Bioinformatics

• Gene Symbol: DSC2
• Uniprot:
  • P55292()