Desmocollin-2 Antibody (7G6) - Azide and BSA Free

Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) - Azide and BSA Free [NBP2-54519] - Formalin-fixed, paraffin-embedded human Skin stained with Desmocollin-2 Antibody (7G6).

Immunohistochemistry-Paraffin: Desmocollin-2 Antibody (7G6) - Azide and BSA Free [NBP2-54519] - Formalin-fixed, paraffin-embedded human Skin stained with Desmocollin-2/3 Monoclonal Antibody (7G6).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 7G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Desmocollin-2 Antibody (7G6) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53378).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human Desmocollin-2 extracellular domain
• Localization: Cell surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DSC2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Western Blot 0.5 - 1.0 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Others not known.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Desmocollin-2 Antibody (7G6) - Azide and BSA Free

• ARVD11
• Cadherin family member 2
• CDHF2
• CDHF2DGII/III
• desmocollin 2
• Desmocollin2
• Desmocollin-2
• Desmocollin-3
• Desmosomal glycoprotein II
• desmosomal glycoprotein II/III
• Desmosomal glycoprotein III
• DG2
• DSC2
• DSC3DKFZp686I11137

Background

Desmocollin 2 is encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11. Alternative splicing results in two transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DSC2