Product Search for MMP9: Novus Biologicals

DMP900

Human MMP-9 Quantikine ELISA Kit

(227 Publications)

1 Kit

DMP900

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	PDMP900
HRP	SMP900

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AF909

MMP-9 Antibody [Unconjugated]

(2 Reviews)

(121 Publications)

100 μg

AF909

HAF019). A specific band was detected for MMP-9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse spleen tissue and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). A specific band was detected for MMP-9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse spleen tissue and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for MMP-9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

MMP-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for MMP-9 at approximately 155 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

MMP-9 was detected in perfusion fixed frozen sections of rat brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

<b>Biological Strategies Validation. </b>Remodeled ECM promotes malignancy of ovarian cancer via FAK-ERK-MMP activation. (a) Specimens from Fig. 5D were immunoassayed for p-FAKY397 (red), and nuclei were stained with Hoechst (blue). (b) IHC of active ERK (p-p44/42 MAPKThr202/Tyr204) in tumor lesions from WT and ApoE−/− mice with PBS or BAPN treatment two weeks after ID8 engraftment. (c) PBS or PD-325901 was administrated to ApoE−/− mice once ID8-Luciferase cells were intraperitoneally injected and treatment continued for one month. The representative images (top) and quantification data (bottom) of MMP-9 protein expression in tumor lesions two months after ID8 engraftment. (d) In vivo luciferase expression was determined two weeks or two months post treatment. Luminescence (right panel) is represented as the radiance (p/s/cm2/sr). Each experiment included data from 4 mice. Bar represents 50 μm. *P < 0.05; **P < 0.005 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29458390), licensed under a CC-BY license. Not internally tested by R&D Systems.

Fibrotic deposit and cardiac dysfunction correlate with decreasing CVPC presence in mdx heart. (A) Representative images of histological analysis stained using Masson trichrome technique showing myocytes (in red) and collagenous fibrotic tissue (in blue) in the left ventricle of WT and mdx hearts at 9, 24, and 52 wo. Line represents 100 µm. (B) The ratio of red and blue stained tissue was evaluated in WT hearts (open bars and black dots, n = 4–11 slices/3 animals per group) and mdx hearts (black bars and grey dots, n = 3–16 slices/3 animals per group) at the age of 24 wo and further at 52 wo. Statistical significance was calculated by Kruskal–Wallis test and Dunn‘s multiple comparison post-hoc test (*** p < 0.001, **** p < 0.0001). (C) Western blot analysis of collagen proteins and inflammatory proteins in the cardiac tissues. Left panel shows representative images of collagen 1A1 (Coll 1A1), collagen 3 (Coll 3), cyclooxygenase 2 (COX-2), and matrix metalloproteinase 9 (MMP-9) compared to the GAPDH control. The right panels show the normalized densitometry of each protein normalized by GAPDH content of WT (open bars, n = 2 animals) and mdx (black bars, n = 2 animals). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34068508), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>MMP2, MMP9, and RANKL expression in AAAs.A. Immunohistochemical staining for indicated proteins of serial sections of aortas one week after CaCl2 treatment. Elastic van Gieson staining is also shown. SM alpha -actin and F4/80 were stained to locate SMCs and macrophages, respectively. Shown are representative images of 4 or more samples in each group. Scale bars, 50 µm. B. Relative positive staining area of MMP2, MMP9, and RANKL in sections from control diet and EPA diet groups. n = 4–5. *P<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0096286), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>BAPN treatment delays ovarian cancer progression by reducing adhesions. (a) Experimental design: PBS or BAPN was intraperitoneally administrated to 20-weeks-old female ApoE−/− mice each day and continued for four weeks. A cohort of mice was sacrificed for further experiments. For the remaining mice, the drug treatment was stopped for two weeks before the establishment of ID8 allografts. (b) Hydroxyproline was measured in the plasma and diaphragm. (c) Masson’s Trichrome stain after BAPN treatment (left). The positive-staining percentage of 10 random fields was calculated (right). Bar represents 50 μm. (d) Cells adhesive to the omentum were analyzed four hours after ID8 intraperitoneal injection by fluorescence microscopy (left). The adhesive cells were determined from the total fluorescent intensity after digestion (right). Bar represents 200 μm. (e) In vivo luciferase measured at two weeks (top) and two months (bottom) post establishment in ApoE−/− mice with PBS or BAPN pre-treatment. Quantification of luminescence is represented as the radiance. (f) MMP-9 expression measured by IHC in tumor lesions of ApoE−/− mice with PBS or BAPN treatment. Each experiment includes data from 4 mice. Bar represents 50 μm. *P < 0.05; **P < 0.005 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29458390), licensed under a CC-BY license. Not internally tested by R&D Systems.

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and macrophages. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP‑9 was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and macrophages. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

$Single cell RNA-sequencing analysis of healing bone fractures. A Preparation of non-haematopoietic stromal cells from PFD14 and control bone for scRNA-seq analysis. b UMAP plots showing colour-coded cell clusters from control and PFD14 bone. Cell types and numbers per cluster are displayed on the right. c–e Heatmap showing the top 5 marker genes for each cluster (c). Feature blots showing markers for non-haematopoietic bone cells: Acan—chondrocytes (CHO); Igfbp6—fibroblasts (FB); Pdgfrb—bone mesenchymal stromal cells (BMSCs); Col22a1—osteoblast lineage cells (OBs); Emcn—ECs; Rgs5—Smooth muscle cells (SMCs) (d). Comparison of marker gene expression in PFD14 and control samples (e). Haematopoietic cells (HCs-1,2,3,4). f, g UMAP plots showing colour-coded BMSC subclusters from control and PFD14 bone (f). Fabp5 and Mmp9 expressing cells are increased in PFD14 samples (g). h, i UMAP plot showing mpMSCs and SCs in the control and PFD14 mpMSC subcluster (h). Fracture-derived SCs show higher expression of Mmp9 and Fabp5 (i). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35091558), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF909U-100UG
Alexa Fluor 405	AF909V-100UG
Alexa Fluor 488	AF909G-100UG
Alexa Fluor 532	AF909X-100UG
Alexa Fluor 594	AF909T-100UG
Alexa Fluor 647	AF909R-100UG
Alexa Fluor 700	AF909N-100UG
Alexa Fluor 750	AF909S-100UG
Alexa Fluor Plus 405	AF909AFP405-100UG
Alexa Fluor Plus 488	AF909AFP488-100UG
Alexa Fluor Plus 555	AF909AFP555-100UG
Alexa Fluor Plus 594	AF909AFP594-100UG
Alexa Fluor Plus 647	AF909AFP647-100UG
Alexa Fluor Plus 680	AF909AFP680-100UG
Allophycocyanin	AF909A
Biotin	BAF909
CoraFluor 1	AF909CL1
DyLight 350	AF909D
DyLight 405	AF909E
DyLight 488	AF909K
DyLight 550	AF909L
DyLight 594	AF909M
DyLight 650	AF909W
DyLight 680	AF909Y
DyLight 755	AF909Z
FITC	AF909F
HRP	AF909H
Janelia Fluor 525	AF909JF525
Janelia Fluor 549	AF909I
Janelia Fluor 646	AF909J
Janelia Fluor 669	AF909JF669
PE	AF909P
PerCP	AF909C
mFluor Violet 450 SE	AF909MFV450
mFluor Violet 500 SE	AF909MFV500
mFluor Violet 610 SE	AF909MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF909-SP

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DY911

Human MMP-9 DuoSet ELISA, 15 Plate

(81 Publications)

1 Kit (for 5 Plates)

DY911

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-13173

MMP-9 Antibody (4A3) - BSA Free

(2 Reviews)

(37 Publications)

0.1 ml

NBP2-13173

<b>Biological Strategies Validation. </b>MMP-9-Antibody-4A3-Western-Blot-NBP2-13173-img0011.jpg

Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE tissue section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary antibody and DAB reagent followed by hematoxylin counterstaining. This antibody generated a specific extracellular and cytoplasmic staining primarily in the cancer cells while the signal was pretty weak in the tumor stroma.

Immunohistochemistry-Paraffin: MMP-9 Antibody (4A3) [NBP2-13173] - Analysis of a FFPE section of human esophageal cancer using MMP9 antibody (clone 4A3) at 1:200. The staining was developed with HRP-labelled secondary antibody and DAB reagent followed by hematoxylin counterstaining. This antibody clone generated an extracellular and cytoplasmic staining mainly in the cancer cells while the signal was very weak in the stromal cells of the tumor.

Western Blot: MMP-9 Antibody (4A3) [NBP2-13173] - 2-DZ analysis & identification of MMP-9 by Western blot. Left panels (A & B): 2-D zymography (2-DZ) of serum samples from healthy control (A) & from an inactive RR-MS patient not subjected to therapy (same patient as in Fig. 2; B). For 2-DZ, aliquots of 35 μl of serum were resuspended in the rehydration solution & subjected to isoelectrofocusing (IEF; 1st dimension) on IPG Dry-Strips of 13 cm in a linear pH gradient of 4–7. After IEF, IPG strips were equilibrated & then applied for the 2nd dimension in a 8.5% (w/v) polyacrylamide gel copolymerized with 0.1% (w/v) gelatin. The isoforms & charge variants of MMP-2 & MMP-9 appear as clear spots of digestion on the dark background of the gel. Right panels (A' & B') represent Western blot analysis of the same sera shown in A & B. Aliquots of 35 μl of serum samples (instead of the usual 20 μl) were subjected to 2D electrophoresis (2-DE) by using 8.5% (w/v) polyacrylamide gels without gelatin. After transfer of the proteins, the nitrocellulose membranes were incubated with an anti MMP-9 (Ab-8) Mouse mAb (IA5) at concentration of 2.66 μg/ml. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24616914), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Histological and immunohistochemistry results of periapical lesions in each group. (A) Representative histopathological images in periapical areas after root canal filling; (B) The inflammation grade evaluation of periapical lesions (n = 10); (C) Representative immunohistochemistry (IHC) staining of MMP-9 in periapical areas after root canal filling. Green boxes indicate peripheral periodontal tissue; (D) Representative immunohistochemistry (IHC) staining of TNF-alpha in periapical areas after root canal filling. Green boxes indicate peripheral periodontal tissue. ns p > 0.05, not significant, * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36361925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Ca, Eq, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca, Eq, Pm, Rb

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-13173AF350
Alexa Fluor 405	NBP2-13173AF405
Alexa Fluor 488	NBP2-13173AF488
Alexa Fluor 532	NBP2-13173AF532
Alexa Fluor 594	NBP2-13173AF594
Alexa Fluor 647	NBP2-13173AF647
Alexa Fluor 700	NBP2-13173AF700
Alexa Fluor 750	NBP2-13173AF750
Alexa Fluor Plus 405	NBP2-13173AFP405
Alexa Fluor Plus 488	NBP2-13173AFP488
Alexa Fluor Plus 555	NBP2-13173AFP555
Alexa Fluor Plus 594	NBP2-13173AFP594
Alexa Fluor Plus 647	NBP2-13173AFP647
Alexa Fluor Plus 680	NBP2-13173AFP680
Allophycocyanin	NBP2-13173APC
Biotin	NBP2-13173B
CoraFluor 1	NBP2-13173CL1
DyLight 350	NBP2-13173UV
DyLight 405	NBP2-13173V
DyLight 488	NBP2-13173G
DyLight 550	NBP2-13173R
DyLight 594	NBP2-13173DL594
DyLight 650	NBP2-13173C
DyLight 680	NBP2-13173FR
DyLight 755	NBP2-13173IR
FITC	NBP2-13173F
HRP	NBP2-13173H
Janelia Fluor 525	NBP2-13173JF525
Janelia Fluor 549	NBP2-13173JF549
Janelia Fluor 646	NBP2-13173JF646
Janelia Fluor 669	NBP2-13173JF669
PE	NBP2-13173PE
PerCP	NBP2-13173PCP
mFluor Violet 450 SE	NBP2-13173MFV450
mFluor Violet 500 SE	NBP2-13173MFV500
mFluor Violet 610 SE	NBP2-13173MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-13173SS
Azide and BSA Free	NBP2-80855

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MMPT90

Mouse Total MMP-9 Quantikine ELISA Kit

(42 Publications)

1 Kit

MMPT90

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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AF911

MMP-9 Antibody [Unconjugated]

(24 Publications)

100 μg

AF911

Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

MMP‑9 was detected in immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 using 5 µg/mL Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for <a class=

Fluorescent ICC Staining of Cells on Coverslips." alt="MMP‑9 was detected in immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 using 5 µg/mL Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

MMP-9 was detected in immersion fixed MCF-7 human breast cancer cell line using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # <a class=

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) according to increasing plasma MMP-9:TIMP-1 levels; Q1 being the group with the lowest level. Image collected and cropped by CiteAb from the following open publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Co-culture of THP1-derived macrophages with Panc89 hA8 WT and KO cells. (A) The schematic model depicts the interactions of THP1-derived macrophages (green, M0) and Panc89 cells with or without ADAM8 (red). Created with BioRender.com. (B) ADAM8 mRNA expression in both Panc89 hA8 WT and KO is not affected by M0, whereas LCN2 mRNA expression. Data are presented as mean values ± S.D. *** p < 0.001. (C) is upregulated after co-culture in an ADAM8-dependent manner. Data are presented as mean values ± S.D. *** p < 0.001. (D) The graph illustrates the upregulation of MMP-9 mRNA expression in both Panc89 hA8 WT and KO after co-culture (n = 2). Data are presented as mean values ± S.D. *** p < 0.001. (E) Representative immunoblot shows the detection of ADAM8, MMP-9, and LCN2 with or without co-culture. In addition to the qPCR results, MMP-9 and LCN2 are upregulated after co-culture at the protein level (n = 2). (F) Representative zymography of Panc89 hA8 WT and KO cells with or without co-culture demonstrates less active MMP-9 in Panc89 hA8 KO cells than in Panc89 hA8 WT cells after co-culture. (G) Quantification of active MMP-9 refers to total MMP-9 in zymography of Panc89 hA8 WT and KO cells after co-culture (n = 2). Data are presented as mean values ± S.D. * p < 0.05. Representative images of Panc89 cells before and after co-culture are shown in (H); scale bar, 100 μm. After co-culture, morphological changes are visible in both Panc89 hA8 WT and KO cells. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35216088), licensed under a CC-BY license. Not internally tested by R&D Systems.

ADAM8 and LCN2 levels correlate in Panc89- and MB-231-derived extracellular vesicles (EV). Representative Western blots (A) of EVs derived from either Panc89 hA8 WT or KO cells, and cell lysate (CL) of Panc89 hA8 WT cells, and (B) of EVs derived from either MB-231 hA8 WT or MB-231 KO cells show the detection of ADAM8, MMP-9, LCN2, and Flotillin-1 in the upper part. Diagrams below illustrate the quantification and downregulation of LCN2 secretion (relative to Flotillin-1 secretion) in EVs isolated from Panc89 (hA8 WT or hA8 KO 1) or MB-231 (hA8 WT or hA8 KO) cells (n = 3). Data are presented as mean values ± S.D. ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35216088), licensed under a CC-BY license. Not internally tested by R&D Systems.

Co-culture of THP1-derived and polarized macrophages with Panc89 hA8 WT and KO cells. (A) Western blot illustrates the detection of ADAM8, MMP-9, and LCN2 in Panc89 hA8 WT and KO control cells (Ø) and after co-culture with M0, M1, and M2 macrophages (two time points: 0 h and 1 h). ADAM8 is upregulated in Panc89 hA8 WT cells after co-culture with M2-polarized macrophages. Panc89 cells show the highest MMP-9 expression after co-culture with M0, but M1 macrophages also upregulate MMP-9. LCN2 is dependent on ADAM8 when upregulated in Panc89 cells after co-culture with M0 and M2 macrophages but independent of ADAM8 in Panc89 cells co-cultured with M1 macrophages. (B) ADAM8, (C) MMP-9, and (D) LCN2 ELISA of Panc89 hA8 WT and KO cell-derived supernatants of control cells and after co-culture with M0, M1, and M2 (two time points: 0 h and 1 h). In accordance with the immunoblot results of (A), ADAM8 is upregulated in supernatants derived from Panc89 hA8 WT cells after co-culture with M2 macrophages (B). At the same time, macrophages increase MMP-9 secretion from an undetectable level to almost 80,000 pg/mL in Panc89 hA8 WT and 60,000 pg/mL in Panc89 hA8 KO cells. M1 and M2 macrophages increase MMP-9 secretion of Panc89 independent of ADAM8, but not as high as in Panc89 cells co-cultured with M0. In contrast, LCN2 is upregulated in Panc89 hA8 WT cells by M0 and M1, but not by M2 macrophages. In the absence of ADAM8, Panc89 hA8 KO cells show low LCN2 secretion in control cells and after co-culture with M0 and M2 macrophages. Only after co-culture with M1 macrophages is the LCN2 secretion level increased (n = 1). Data are presented as mean values ± S.D. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35216088), licensed under a CC-BY license. Not internally tested by R&D Systems.

911-MP) was serially diluted and captured by Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human MMP‑9 (Catalog # <a class=

911-MP) was serially diluted and captured by Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF911U-100UG
Alexa Fluor 405	AF911V-100UG
Alexa Fluor 488	AF911G-100UG
Alexa Fluor 532	AF911X-100UG
Alexa Fluor 594	AF911T-100UG
Alexa Fluor 647	AF911R-100UG
Alexa Fluor 700	AF911N-100UG
Alexa Fluor 750	AF911S-100UG
Alexa Fluor Plus 405	AF911AFP405-100UG
Alexa Fluor Plus 488	AF911AFP488-100UG
Alexa Fluor Plus 555	AF911AFP555-100UG
Alexa Fluor Plus 594	AF911AFP594-100UG
Alexa Fluor Plus 647	AF911AFP647-100UG
Alexa Fluor Plus 680	AF911AFP680-100UG
Allophycocyanin	AF911A
Biotin	BAF911
CoraFluor 1	AF911CL1
DyLight 350	AF911D
DyLight 405	AF911E
DyLight 488	AF911K
DyLight 550	AF911L
DyLight 594	AF911M
DyLight 650	AF911W
DyLight 680	AF911Y
DyLight 755	AF911Z
FITC	AF911F
HRP	AF911H
Janelia Fluor 525	AF911JF525
Janelia Fluor 549	AF911I
Janelia Fluor 646	AF911J
Janelia Fluor 669	AF911JF669
PE	AF911P
PerCP	AF911C
mFluor Violet 450 SE	AF911MFV450
mFluor Violet 500 SE	AF911MFV500
mFluor Violet 610 SE	AF911MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF911-SP

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911-MP

Recombinant Human MMP-9 Protein, CF

(32 Publications)

10 μg

911-MP

ES001)." class="big_lightbox">

Recombinant Human MMP-9 (Catalog # 911-MP) is measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # <a class=

ES001)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP1-57940

MMP-9 Antibody - BSA Free

(4 Reviews)

(10 Publications)

0.1 mg

NBP1-57940

Western Blot: MMP-9 Antibody [NBP1-57940] - Analysis of MMP-9 in mouse brain tumor lysate (GL261 implanted mouse glioma tissue, 40 ug/lane) using anti-MMP-9 antibody. Image from verified customer review.

Western Blot: MMP-9 Antibody [NBP1-57940] - HeLa cell lysate. Antibody at 1.25 ug/mL.

Western Blot: MMP-9 Antibody [NBP1-57940] - Human Fetal Lung. Antibody at 1.0 ug/mL.

Reactivity:

Hu, Mu, Rt, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Sh

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-57940AF350
Alexa Fluor 405	NBP1-57940AF405
Alexa Fluor 488	NBP1-57940AF488
Alexa Fluor 532	NBP1-57940AF532
Alexa Fluor 594	NBP1-57940AF594
Alexa Fluor 647	NBP1-57940AF647
Alexa Fluor 700	NBP1-57940AF700
Alexa Fluor 750	NBP1-57940AF750
Alexa Fluor Plus 405	NBP1-57940AFP405
Alexa Fluor Plus 488	NBP1-57940AFP488
Alexa Fluor Plus 555	NBP1-57940AFP555
Alexa Fluor Plus 594	NBP1-57940AFP594
Alexa Fluor Plus 647	NBP1-57940AFP647
Alexa Fluor Plus 680	NBP1-57940AFP680
Allophycocyanin	NBP1-57940APC
Biotin	NBP1-57940B
CoraFluor 1	NBP1-57940CL1
DyLight 350	NBP1-57940UV
DyLight 405	NBP1-57940V
DyLight 488	NBP1-57940G
DyLight 550	NBP1-57940R
DyLight 594	NBP1-57940DL594
DyLight 650	NBP1-57940C
DyLight 680	NBP1-57940FR
DyLight 755	NBP1-57940IR
FITC	NBP1-57940F
HRP	NBP1-57940H
Janelia Fluor 525	NBP1-57940JF525
Janelia Fluor 549	NBP1-57940JF549
Janelia Fluor 646	NBP1-57940JF646
Janelia Fluor 669	NBP1-57940JF669
PE	NBP1-57940PE
PerCP	NBP1-57940PCP
mFluor Violet 450 SE	NBP1-57940MFV450
mFluor Violet 500 SE	NBP1-57940MFV500
mFluor Violet 610 SE	NBP1-57940MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-57940SS

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MAB936

MMP-9 Antibody (36020) [Unconjugated]

(15 Publications)

100 μg

MAB936

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

MMP-9 was detected in immersion fixed SW1353 human chondrosarcoma cell line using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="MMP-9 was detected in immersion fixed SW1353 human chondrosarcoma cell line using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

MMP-9 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

911-MP) was serially diluted and captured by Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

911-MP) was serially diluted and captured by Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB936U-100UG
Alexa Fluor 405	FAB936V-100UG
Alexa Fluor 488	FAB936G-100UG
Alexa Fluor 532	FAB936X-100UG
Alexa Fluor 594	FAB936T-100UG
Alexa Fluor 647	FAB936R-100UG
Alexa Fluor 700	FAB936N-100UG
Alexa Fluor 750	FAB936S-100UG
Alexa Fluor Plus 405	FAB936AFP405-100UG
Alexa Fluor Plus 488	FAB936AFP488-100UG
Alexa Fluor Plus 555	FAB936AFP555-100UG
Alexa Fluor Plus 594	FAB936AFP594-100UG
Alexa Fluor Plus 647	FAB936AFP647-100UG
Alexa Fluor Plus 680	FAB936AFP680-100UG
Allophycocyanin	FAB936A
Biotin	FAB936B
CoraFluor 1	FAB936CL1
DyLight 350	FAB936D
DyLight 405	FAB936E
DyLight 488	FAB936K
DyLight 550	FAB936L
DyLight 594	FAB936M
DyLight 650	FAB936W
DyLight 680	FAB936Y
DyLight 755	FAB936Z
FITC	FAB936F
HRP	FAB936H
Janelia Fluor 525	FAB936JF525
Janelia Fluor 549	FAB936I
Janelia Fluor 646	FAB936J
Janelia Fluor 669	FAB936JF669
PE	FAB936P
PerCP	FAB936C
mFluor Violet 450 SE	FAB936MFV450
mFluor Violet 500 SE	FAB936MFV500
mFluor Violet 610 SE	FAB936MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB936-SP
Carrier Free	MAB936-500

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909-MM

Recombinant Mouse MMP-9 Protein, CF

(20 Publications)

10 μg

909-MM

ES001)." class="big_lightbox">

ES001)." alt="Recombinant Mouse MMP-9 (Catalog # 909-MM) is measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001)." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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RMP900

Rat Total MMP-9 Quantikine ELISA Kit

(15 Publications)

1 Kit

RMP900

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

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MMP900B

Mouse Pro-MMP-9 Quantikine ELISA Kit

(15 Publications)

1 Kit

MMP900B

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Human MMP-9 Quantikine ELISA Kit

MMP-9 Antibody [Unconjugated]

Human MMP-9 DuoSet ELISA, 15 Plate

MMP-9 Antibody (4A3) - BSA Free

Mouse Total MMP-9 Quantikine ELISA Kit

MMP-9 Antibody [Unconjugated]

Recombinant Human MMP-9 Protein, CF

MMP-9 Antibody - BSA Free

MMP-9 Antibody (36020) [Unconjugated]

Recombinant Mouse MMP-9 Protein, CF

Rat Total MMP-9 Quantikine ELISA Kit

Mouse Pro-MMP-9 Quantikine ELISA Kit