Recombinant Mouse MMP-9 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Mouse MMP-9 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001). The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived mouse MMP-9 protein
Ala20-Pro730
Accession #
N-terminal Sequence
Ala20
Structure / Form
Pro form
Protein/Peptide Type
Recombinant Enzymes
Gene
Mmp9
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-105 kDa, reducing conditions
Publications
Read Publications using
909-MM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and Brij-35.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5 (TCNB)
  • Recombinant Mouse MMP-9 (rmMMP-9) (Catalog # 909-MM)
  • p-aminophenylmercuric acetate (APMA) (Sigma, Catalog # A-9563), 100 mM stock in DMSO
  • Substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 (Catalog # ES001), 2 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rmMMP-9 to 100 µg/mL in Assay Buffer.
  2. Activate rmMMP-9 by adding APMA to a final concentration of 1 mM.
  3. Incubate at 37 °C for 2 hours.
  4. Dilute activated rmMMP-9 to 0.4 ng/µL in Assay Buffer.
  5. Dilute Substrate to 20 µM in Assay Buffer.
  6. Load into a black well plate 50 µL of the 0.4 ng/µL rmMMP-9 and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate without any rmMMP-9.
  7. Read at excitation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes.
  8. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

Per Well:
  • rmMMP-9: 0.02 µg
  • Substrate: 10 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MMP-9 Protein, CF

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the Recombinant Human MMP‑9 (Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

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909-MM
Species: Mu
Applications: Enzyme Activity

Publications for MMP-9 (909-MM)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 8 applications: Bioassay, EnzAct, Enzyme Digestion, In Vivo, WB Ctrl, Zymography, Zymography Positive Ctrl, cell culture.


Filter By Application
Bioassay
(2)
EnzAct
(1)
Enzyme Digestion
(1)
In Vivo
(2)
WB Ctrl
(2)
Zymography
(1)
Zymography Positive Ctrl
(2)
cell culture
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(7)
Rat
(1)
N/A
(3)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 909-MM Applications Species
YY Jo, H Kweon, DW Kim, MK Kim, SG Kim, JY Kim, WS Chae, SP Hong, YH Park, SY Lee, JY Choi Accelerated biodegradation of silk sutures through matrix metalloproteinase activation by incorporating 4-hexylresorcinol Sci Rep, 2017;7(0):42441. 2017 [PMID: 28205580] (Enzyme Digestion, Human) Enzyme Digestion Human
G Yuan, L Chen, J Feng, G Yang, Q Ni, X Xu, C Wan, M Lindsey, KJ Donly, M MacDougall, Z Chen, S Chen Dentin Sialoprotein is a Novel Substrate of Matrix Metalloproteinase 9 in vitro and in vivo Sci Rep, 2017;7(0):42449. 2017 [PMID: 28195206] (Zymography, Mouse) Zymography Mouse
Reversal of TREM-1 ectodomain shedding and improved bacterial clearance by intranasal metalloproteinase inhibitors Mucosal Immunol, 2016;0(0):. 2016 [PMID: 27966555] (Bioassay, Mouse) Bioassay Mouse
MH Park, JK Lee, N Kim, WK Min, JE Lee, KT Kim, H Akiyama, H Herzog, EH Schuchman, HK Jin, JS Bae Neuropeptide Y induces hematopoietic stem/progenitor cell mobilization by regulating matrix metalloproteinase-9 activity through Y1 receptor in osteoblasts Stem Cells, 2016;0(0):. 2016 [PMID: 27090492] (In Vivo, Mouse) In Vivo Mouse
Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J Immunol, 2013;190(10):5329-36. 2013 [PMID: 23589623] (WB Ctrl, Mouse) WB Ctrl Mouse
Song J, Wu C, Zhang X, Sorokin L In vivo processing of CXCL5 (LIX) by matrix metalloproteinase (MMP)-2 and MMP-9 promotes early neutrophil recruitment in IL-1beta-induced peritonitis. J Immunol, 2013;190(1):401-10. 2013 [PMID: 23225890] (cell culture, Mouse) cell culture Mouse
Suryawanshi A, Mulik S, Sharma S Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor. J. Immunol., 2011;186(6):3653-65. 2011 [PMID: 21325621] (EnzAct, Mouse) EnzAct Mouse
Le Quement C, Guenon I, Gillon JY, Valenca S, Cayron-Elizondo V, Lagente V, Boichot E The selective MMP-12 inhibitor, AS111793 reduces airway inflammation in mice exposed to cigarette smoke. Br. J. Pharmacol., 2008;154(6):1206-15. 2008 [PMID: 18493250] (Zymography Positive Ctrl, N/A) Zymography Positive Ctrl N/A
Gaggar A, Jackson PL, Noerager BD, O'Reilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation. J. Immunol., 2008;180(8):5662-9. 2008 [PMID: 18390751] (In Vivo, Mouse) In Vivo Mouse
Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Blood, 2007;109(5):2234-42. 2007 [PMID: 17082320] (WB Ctrl, N/A) WB Ctrl N/A
Show All 12 Publications.

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FAQs for MMP-9 (909-MM). (Showing 1 - 1 of 1 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)

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Bioinformatics

Gene Symbol Mmp9
Uniprot