Recombinant Mouse MMP-9 Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Mouse MMP-9 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001). The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived mouse MMP-9 protein
Accession #
N-terminal Sequence
Structure / Form
Pro form
Protein/Peptide Type
Recombinant Enzymes
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
80-105 kDa, reducing conditions
Read Publications using
909-MM in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and Brij-35.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5 (TCNB)
  • Recombinant Mouse MMP-9 (rmMMP-9) (Catalog # 909-MM)
  • p-aminophenylmercuric acetate (APMA) (Sigma, Catalog # A-9563), 100 mM stock in DMSO
  • Substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 (Catalog # ES001), 2 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rmMMP-9 to 100 µg/mL in Assay Buffer.
  2. Activate rmMMP-9 by adding APMA to a final concentration of 1 mM.
  3. Incubate at 37 °C for 2 hours.
  4. Dilute activated rmMMP-9 to 0.4 ng/µL in Assay Buffer.
  5. Dilute Substrate to 20 µM in Assay Buffer.
  6. Load into a black well plate 50 µL of the 0.4 ng/µL rmMMP-9 and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 20 µM Substrate without any rmMMP-9.
  7. Read at excitation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes.
  8. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

Per Well:
  • rmMMP-9: 0.02 µg
  • Substrate: 10 µM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MMP-9 Protein, CF

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase


Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the Recombinant Human MMP‑9 (Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

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Applications: Enzyme Activity

Publications for MMP-9 (909-MM)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 8 applications: Bioassay, EnzAct, Enzyme Digestion, In Vivo, WB Ctrl, Zymography, Zymography Positive Ctrl, cell culture.

Filter By Application
Enzyme Digestion
In Vivo
WB Ctrl
Zymography Positive Ctrl
cell culture
All Applications
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All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 909-MM Applications Species
YY Jo, H Kweon, DW Kim, MK Kim, SG Kim, JY Kim, WS Chae, SP Hong, YH Park, SY Lee, JY Choi Accelerated biodegradation of silk sutures through matrix metalloproteinase activation by incorporating 4-hexylresorcinol Sci Rep, 2017;7(0):42441. 2017 [PMID: 28205580] (Enzyme Digestion, Human) Enzyme Digestion Human
G Yuan, L Chen, J Feng, G Yang, Q Ni, X Xu, C Wan, M Lindsey, KJ Donly, M MacDougall, Z Chen, S Chen Dentin Sialoprotein is a Novel Substrate of Matrix Metalloproteinase 9 in vitro and in vivo Sci Rep, 2017;7(0):42449. 2017 [PMID: 28195206] (Zymography, Mouse) Zymography Mouse
Reversal of TREM-1 ectodomain shedding and improved bacterial clearance by intranasal metalloproteinase inhibitors Mucosal Immunol, 2016;0(0):. 2016 [PMID: 27966555] (Bioassay, Mouse) Bioassay Mouse
MH Park, JK Lee, N Kim, WK Min, JE Lee, KT Kim, H Akiyama, H Herzog, EH Schuchman, HK Jin, JS Bae Neuropeptide Y induces hematopoietic stem/progenitor cell mobilization by regulating matrix metalloproteinase-9 activity through Y1 receptor in osteoblasts Stem Cells, 2016;0(0):. 2016 [PMID: 27090492] (In Vivo, Mouse) In Vivo Mouse
Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J Immunol, 2013;190(10):5329-36. 2013 [PMID: 23589623] (WB Ctrl, Mouse) WB Ctrl Mouse
Song J, Wu C, Zhang X, Sorokin L In vivo processing of CXCL5 (LIX) by matrix metalloproteinase (MMP)-2 and MMP-9 promotes early neutrophil recruitment in IL-1beta-induced peritonitis. J Immunol, 2013;190(1):401-10. 2013 [PMID: 23225890] (cell culture, Mouse) cell culture Mouse
Suryawanshi A, Mulik S, Sharma S Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor. J. Immunol., 2011;186(6):3653-65. 2011 [PMID: 21325621] (EnzAct, Mouse) EnzAct Mouse
Le Quement C, Guenon I, Gillon JY, Valenca S, Cayron-Elizondo V, Lagente V, Boichot E The selective MMP-12 inhibitor, AS111793 reduces airway inflammation in mice exposed to cigarette smoke. Br. J. Pharmacol., 2008;154(6):1206-15. 2008 [PMID: 18493250] (Zymography Positive Ctrl, N/A) Zymography Positive Ctrl N/A
Gaggar A, Jackson PL, Noerager BD, O'Reilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation. J. Immunol., 2008;180(8):5662-9. 2008 [PMID: 18390751] (In Vivo, Mouse) In Vivo Mouse
Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Blood, 2007;109(5):2234-42. 2007 [PMID: 17082320] (WB Ctrl, N/A) WB Ctrl N/A
Show All 12 Publications.

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FAQs for MMP-9 (909-MM). (Showing 1 - 1 of 1 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA. looks like 2 have been tested for capture (please note the tested species for each of these). has been tested for detection.

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Gene Symbol Mmp9