ZW10 Antibody (2E5)

Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5. Lane 1: ZW10 transfected lysatE (88.8 KDa). Lane 2: Non-transfected lysate.

Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi or non-transfected control. Blot probed with H00009183-M05. GAPDH (36.1 kDa) used as loading control.

ELISA: ZW10 Antibody (2E5) [H00009183-M05] - Detection limit for recombinant GST tagged ZW10 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 2E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ZW10 Antibody (2E5) Summary

• Immunogen: ZW10 (NP_004715, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. masfvtevla hsgrlekedl gtrisrltrr veeikgevcn miskkysefl psmqsaqgli tqvdklsedi dllksriese vrrdlhvstg eftdlkqqle
• Specificity: ZW10 (2E5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZW10
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZW10 Antibody (2E5)

• E3 ubiquitin-protein ligase ZSWIM2
• EC 6.3.2.-
• MEKK1-related protein X
• MEX
• MGC33890
• Zinc finger SWIM domain-containing protein 2
• zinc finger, SWIM domain containing 2
• zinc finger, SWIM-type containing 2
• ZZZ2ZZ-type zinc finger-containing protein 2

Background

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZW10
• Entrez:
  • 9183(Human)
• OMIM:
  • 603954(Human)
• Uniprot:
  • NP_004715(Human)