ZPBP Antibody - Azide and BSA Free

Western Blot: ZPBP Antibody [NBP2-93039] - Analysis of extracts of Mouse brain, using ZPBP at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:

Immunohistochemistry-Paraffin: ZPBP Antibody [NBP2-93039] - Immunohistochemistry of paraffin-embedded Rat ovary using ZPBP Rabbit pAb (NBP2-93039) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry: ZPBP Antibody [NBP2-93039] - Analysis of mouse testis using ZPBP at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: ZPBP Antibody [NBP2-93039] - Immunohistochemistry of paraffin-embedded Human placenta using ZPBP Rabbit pAb (NBP2-93039) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence: ZPBP Antibody - Azide and BSA Free [NBP2-93039] - Immunofluorescence analysis of paraffin-embedded rat testis using ZPBP Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ZPBP Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 45-200 of human ZPBP (NP_008940.2). HLVRLPRAFRLTKDSVKIVGSTSFPVKAYVMLHQKSPHVLCVTQQLRNAELIDPSFQWYGPKGKVVSVENRTAQITSTGSLVFQNFEESMSGIYTCFLEYKPTVEEIVKRLQLKYAIYAYREPHYYYQFTARYHAAPCNSIYNISFEKKLLQILSK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZPBP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for ZPBP Antibody - Azide and BSA Free

• Iam38
• KIAA0395TIX1
• Sp38
• triple homeobox 1
• Triple homeobox protein 1
• Zinc finger and homeodomain protein 3
• zinc fingers and homeoboxes 3
• zinc fingers and homeoboxes protein 3
• ZPBP
• ZPBP1

Background

ZPBP is one of several proteins that are thought to participate in secondary binding between acrosome-reacted sperm and the egg-specific extracellular matrix, the zona pellucida (McLeskey et al., 1998 (PubMed 9378618)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ZPBP