![Western Blot: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF394 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiZNF394.(1:2000)](http://images.novusbio.com/fullsize/ZNF394-Antibody-OTI1G9-Azide-and-BSA-Free-Western-Blot-NBP2-74949-img0001.jpg "Western Blot: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF394 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiZNF394.(1:2000)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI1G9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Description | Novus Biologicals Mouse ZNF394 Antibody (OTI1G9) - Azide and BSA Free (NBP2-46446) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-296 of human ZNF394 (NP_115540) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ZNF394 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 64.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ZNF394 |
![Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ZNF394 mouse monoclonal antibody. (Heat induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/ZNF394-Antibody-OTI1G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74949-img0007.jpg "Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ZNF394 mouse monoclonal antibody. (Heat induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)")
![Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/ZNF394-Antibody-OTI1G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74949-img0002.jpg "Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)")
![Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ZNF394 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/ZNF394-Antibody-OTI1G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74949-img0003.jpg "Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ZNF394 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)")
![Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/ZNF394-Antibody-OTI1G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74949-img0004.jpg "Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)")
![Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/ZNF394-Antibody-OTI1G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74949-img0005.jpg "Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)")
![Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/ZNF394-Antibody-OTI1G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74949-img0006.jpg "Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)")
![Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Human lymphoma tissue using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/ZNF394-Antibody-OTI1G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74949-img0008.jpg "Immunohistochemistry: ZNF394 Antibody (OTI1G9) - Azide and BSA Free [NBP2-74949] - Staining of paraffin-embedded Human lymphoma tissue using anti-ZNF394 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")