![Western Blot: ZNF295 Antibody [NB100-74655] - Detection of Human ZNF295 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF295 antibody NB100-74655 used for IP at 3 mcg/mg lysate. ZNF295 was also immunoprecipitated by rabbit anti-ZNF295 antibody NB100-74656, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/ZNF295-Antibody-Western-Blot-NB100-74655-img0001.jpg "Western Blot: ZNF295 Antibody [NB100-74655] - Detection of Human ZNF295 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF295 antibody NB100-74655 used for IP at 3 mcg/mg lysate. ZNF295 was also immunoprecipitated by rabbit anti-ZNF295 antibody NB100-74656, which recognizes a downstream epitope.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC, IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit ZNF295 Antibody - BSA Free (NB100-74655) is a polyclonal antibody validated for use in IHC, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 875 and 925 of human zinc finger protein 295 using the numbering given in entry NP_065778.3 (GeneID 49854). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ZNF295 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is not recommended for Western Blot analysis. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ZNF295 |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: ZNF295 Antibody [NB100-74655] - Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100](http://images.novusbio.com/fullsize/ZNF295-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74655-img0003.jpg "Immunocytochemistry/Immunofluorescence: ZNF295 Antibody [NB100-74655] - Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100")
![Immunohistochemistry: ZNF295 Antibody [NB100-74655] - Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZNF295 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5ug/ml).](http://images.novusbio.com/fullsize/ZNF295-Antibody-Immunohistochemistry-NB100-74655-img0002.jpg "Immunohistochemistry: ZNF295 Antibody [NB100-74655] - Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZNF295 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5ug/ml).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")