ZNF24 Antibody [Unconjugated]

ZNF24 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human ZNF24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3637) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ZNF24 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ZNF24
  Ser2-Val368
  Accession # NP_008896
• Specificity: Detects human ZNF24 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ZNF24
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZNF24 Antibody [Unconjugated]

• FLJ32104
• KOX17
• RSG-A
• Zfp191
• zinc finger protein 488
• ZNF24
• ZSCAN3

Background

Human ZNF24, also known as ZNF191, is a 42 kDa transcription regulator that belongs to the Kruppel-like zinc finger gene family. It contains an N-terminal SCAN box domain and four C-terminal tandem zinc finger motifs. ZNF24 is ubiquitously expressed. A second splice isoform that contains a 4 residue substitution for aa 191‑368 of full-length ZNF24, has been reported. Human and mouse ZNF24 share 95% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF24 Antibody (AF3637)(2)

Publications using AF3637

• Pierre Janssen, Irene Tosi, Alexandre Hego, Pauline Maréchal, Thomas Marichal, Coraline Radermecker Neutrophil Extracellular Traps Are Found in Bronchoalveolar Lavage Fluids of Horses With Severe Asthma and Correlate With Asthma Severity Frontiers in Immunology 7/13/2022 [PMID: 35911691]
• Jia , Di, Hasso , Sean M, Chan , Joanne, Filingeri , Domenic, D'Amore , Patricia, Rice , Lori, Pampo , Christin, Siemann , Dietmar, Zurakowski , David, Rodig , Scott J, Moses , Marsha A Transcriptional repression of VEGF by ZNF24: mechanistic studies and vascular consequences in vivo. Blood, 2012-12-03;121(4):707-15. 2012-12-03 [PMID: 23212515] (ChIP, Functional Assay, IHC-P, Western Blot, Human, Mouse)

Bioinformatics

• Gene Symbol: ZNF24
• Uniprot:
  • NP_008896()