| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82866-25ul | |||
NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="ZNF24 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human ZNF24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3637) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3637-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97861-50ul | |||
![Western Blot: ZNF24 Antibody [NBP1-82866] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/ZNF24-Antibody-Western-Blot-NBP1-82866-img0009.jpg "Western Blot: ZNF24 Antibody [NBP1-82866] - Analysis in human cell line SCLC-21H.")
![Immunohistochemistry-Paraffin: ZNF24 Antibody [NBP1-82866] - Staining of human lymph node shows strong cytoplasmic and nuclear positivity in subsets of cells.](http://images.novusbio.com/fullsize/ZNF24-Antibody-Immunohistochemistry-Paraffin-NBP1-82866-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ZNF24 Antibody [NBP1-82866] - Analysis in U2OS and HEK cells. siRNA against ZNF24 show the specificity of the antibody. ZNF24 was used 1/500 in TBST 5% milk, 1 hour at room temperature. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ZNF24-Antibody-Western-Blot-NBP1-82866-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ZNF24 Antibody [NBP1-82866] - ZNF24 promoted PD-L1 expression through binding to its promoter in FFA-treated LO2 cells. ZNF24 and PD-L1 expression in LO2 cells pretreated with siRNA against ZNF24, followed by FFA treatment detected by western blot. ∗∗P < 0.01. PD-L1 expression was suppressed following the ZNF24 knockdown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/antibody/nbp1-82866_rabbit-polyclonal-znf24-antibody-knockdown-validated-20920231837.jpg)
![Western Blot: ZNF24 Antibody [NBP1-82866] - ZNF24 promoted PD-L1 expression through binding to its promoter in FFA-treated LO2 cells. (a) A schematic of the target sites (wild & mutant) of ZNF24 in the promoter of PD-L1. (b–d) Dual-luciferase reporter assays performed in LO2 cells transfected with WT or MT plasmid containing ZNF24-binding sites in the PD-L1 promoter using Lipofectamine 2000 after ZNF24 overexpression. (e–g) ZNF24 expression after FFA treatment determined by qRT-PCR & western blot. (h & i) ZNF24 expression in LO2 cells pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment, measured by western blot. (j & k) ZNF24 & PD-L1 expression in LO2 cells pretreated with siRNA against ZNF24, followed by FFA treatment detected by western blot. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82866_rabbit-polyclonal-znf24-antibody-310202415395912.jpg)
![Western Blot: ZNF24 Antibody [NBP1-82866] - ZNF24 promoted PD-L1 expression through binding to its promoter in FFA-treated LO2 cells. (a) A schematic of the target sites (wild & mutant) of ZNF24 in the promoter of PD-L1. (b–d) Dual-luciferase reporter assays performed in LO2 cells transfected with WT or MT plasmid containing ZNF24-binding sites in the PD-L1 promoter using Lipofectamine 2000 after ZNF24 overexpression. (e–g) ZNF24 expression after FFA treatment determined by qRT-PCR & western blot. (h & i) ZNF24 expression in LO2 cells pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment, measured by western blot. (j & k) ZNF24 & PD-L1 expression in LO2 cells pretreated with siRNA against ZNF24, followed by FFA treatment detected by western blot. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82866_rabbit-polyclonal-znf24-antibody-310202415171324.jpg)
![Western Blot: ZNF24 Antibody [NBP1-82866] - Identification of ZNF24–UBE2I protein interactions. (a & b) Analysis of protein interactions between ZNF24 & UBE2I using the BioGRID database, further confirmed by Co-IP assays. (c–f) UBE2I expression in LO2 cells treated by FFA or pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment measured by western blot. (g & h) After FFA treatment, Sumo-1: ZNF24 & ZNF24 expression levels detected by western blot. (i & j) After UBE2I overexpression, Sumo-1: ZNF24, ZNF24, PD-L1, & UBE2I expression levels were determined by western blot. (k–m) Dual-luciferase reporter assays performed in LO2 cells transfected with WT plasmid containing ZNF24-binding sites in the PD-L1 promoter using Lipofectamine 2000 after ZNF24 overexpression with or without Sumo-1 overexpression. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82866_rabbit-polyclonal-znf24-antibody-310202416121921.jpg)
![Western Blot: ZNF24 Antibody [NBP1-82866] - Identification of ZNF24–UBE2I protein interactions. (a & b) Analysis of protein interactions between ZNF24 & UBE2I using the BioGRID database, further confirmed by Co-IP assays. (c–f) UBE2I expression in LO2 cells treated by FFA or pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment measured by western blot. (g & h) After FFA treatment, Sumo-1: ZNF24 & ZNF24 expression levels detected by western blot. (i & j) After UBE2I overexpression, Sumo-1: ZNF24, ZNF24, PD-L1, & UBE2I expression levels were determined by western blot. (k–m) Dual-luciferase reporter assays performed in LO2 cells transfected with WT plasmid containing ZNF24-binding sites in the PD-L1 promoter using Lipofectamine 2000 after ZNF24 overexpression with or without Sumo-1 overexpression. ∗∗P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35615575), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82866_rabbit-polyclonal-znf24-antibody-31020241612241.jpg)
![Western Blot: ZNF24 Antibody [NBP1-71847] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-ZNF24 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ZNF24 was also immunoprecipitated by rabbit anti-ZNF24 antibody NBP1-71848 which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/ZNF24-Antibody-Western-Blot-NBP1-71847-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ZNF24 Antibody [NBP1-71847] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-ZNF24 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ZNF24 was also immunoprecipitated by rabbit anti-ZNF24 antibody NBP1-71848 which recognizes a downstream epitope.")
![Western Blot: ZNF24 Antibody (2G5) [H00007572-M02] - ZNF24 monoclonal antibody (M02), clone 2G5 Analysis of ZNF24 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ZNF24-Antibody-2G5-Western-Blot-H00007572-M02-img0010.jpg "Western Blot: ZNF24 Antibody (2G5) [H00007572-M02] - ZNF24 monoclonal antibody (M02), clone 2G5 Analysis of ZNF24 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: ZNF24 Antibody (2G5) [H00007572-M02] - Analysis of monoclonal antibody to ZNF24 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ZNF24-Antibody-2G5-Immunocytochemistry-Immunofluorescence-H00007572-M02-img0008.jpg)
![Western Blot: ZNF24 Antibody (2G5) [H00007572-M02] - Analysis of ZNF24 expression in transfected 293T cell line by ZNF24 monoclonal antibody (M02), clone 2G5.Lane 1: ZNF24 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZNF24-Antibody-2G5-Western-Blot-H00007572-M02-img0011.jpg)
![Sandwich ELISA: ZNF24 Antibody (2G5) [H00007572-M02] - Detection limit for recombinant GST tagged ZNF24 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ZNF24-Antibody-2G5-Sandwich-ELISA-H00007572-M02-img0009.jpg)
![Western Blot: ZNF24 Antibody (4C2) [H00007572-M03] - Analysis of ZNF24 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/ZNF24-Antibody-4C2-Western-Blot-H00007572-M03-img0010.jpg "Western Blot: ZNF24 Antibody (4C2) [H00007572-M03] - Analysis of ZNF24 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: ZNF24 Antibody (4C2) [H00007572-M03] - Analysis of monoclonal antibody to ZNF24 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/ZNF24-Antibody-4C2-Immunocytochemistry-Immunofluorescence-H00007572-M03-img0008.jpg)
![Western Blot: ZNF24 Antibody (4C2) [H00007572-M03] - Analysis of ZNF24 expression in transfected 293T cell line by ZNF24 monoclonal antibody (M03), clone 4C2. Lane 1: ZNF24 transfected lysatE (42.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZNF24-Antibody-4C2-Western-Blot-H00007572-M03-img0011.jpg)
![Sandwich ELISA: ZNF24 Antibody (4C2) [H00007572-M03] - Detection limit for recombinant GST tagged ZNF24 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ZNF24-Antibody-4C2-Sandwich-ELISA-H00007572-M03-img0009.jpg)
![Western Blot: ZNF24 Antibody (4A1) [H00007572-M01] - ZNF24 monoclonal antibody (M01), clone 4A1 Analysis of ZNF24 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ZNF24-Antibody-4A1-Western-Blot-H00007572-M01-img0005.jpg "Western Blot: ZNF24 Antibody (4A1) [H00007572-M01] - ZNF24 monoclonal antibody (M01), clone 4A1 Analysis of ZNF24 expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: ZNF24 Antibody (4A1) [H00007572-M01] - Analysis of monoclonal antibody to ZNF24 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 0.1 ug/ml.](http://images.novusbio.com/fullsize/ZNF24-Antibody-4A1-Immunohistochemistry-Paraffin-H00007572-M01-img0004.jpg)
![Western Blot: ZNF24 Antibody (4A1) [H00007572-M01] - Analysis of ZNF24 expression in transfected 293T cell line by ZNF24 monoclonal antibody (M01), clone 4A1.Lane 1: ZNF24 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZNF24-Antibody-4A1-Western-Blot-H00007572-M01-img0006.jpg)
![Western Blot: ZNF24 Antibody [NBP2-97861] - Anti-ZNF24 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293 Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/ZNF24-Antibody-Western-Blot-NBP2-97861-img0002.jpg "Western Blot: ZNF24 Antibody [NBP2-97861] - Anti-ZNF24 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293 Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: ZNF24 Antibody [NBP2-97861] - Immunofluorescence staining of ZNF24 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97861 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97861_rabbit-polyclonal-znf24-antibody-2352025154435.png)
![Western Blot: ZNF24 Antibody [NBP1-71848] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-ZNF24 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ZNF24 was also immunoprecipitated by rabbit anti-ZNF24 antibody NBP1-71847 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/ZNF24-Antibody-Western-Blot-NBP1-71848-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ZNF24 Antibody [NBP1-71848] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-ZNF24 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ZNF24 was also immunoprecipitated by rabbit anti-ZNF24 antibody NBP1-71847 which recognizes an upstream epitope.")
