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ZNF155 Antibody

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Western Blot: ZNF155 Antibody [NBP1-80912] - Analysis in control (vector only transfected HEK293T lysate) and ZNF155 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Western Blot: ZNF155 Antibody [NBP1-80912] - Analysis in control (vector only transfected HEK293T lysate) and ZNF155 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: ZNF155 Antibody [NBP1-80912] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ZNF155 Antibody [NBP1-80912] - Staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ZNF155 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TCHFLREEKFWMMGTATQREGNSGGKIQTELESVPEAGAHEEWSCQQIWEQIAKDLTRSQDSIINNSQFFENGDVPSQVEAGLPTIHTGQKPSQGGKCKQSFSDVPIFDLPQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZNF155
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ZNF155 Protein (NBP1-80912PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ZNF155 Antibody

  • KRAB A domain
  • MGC161655
  • pHZ-96
  • zinc finger protein 155 (pHZ-96)
  • zinc finger protein 155

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). Detection: DAB
Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350] - NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with EPE (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human RCOR1/CoREST Monoclonal Antibody (Catalog # MAB6047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RCOR1/CoREST at approximately 64-66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RCOR1/CoREST was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human RCOR1/CoREST Monoclonal Antibody (Catalog # MAB6047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Immunocytochemistry/Immunofluorescence: ZNF267 Antibody [NBP1-81633] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ZNF267 Antibody [NBP1-81633] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.
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Western Blot: ZNF101 Antibody (2D5) [H00094039-M01] - Analysis of ZNF101 expression in transfected 293T cell line by ZNF101 monoclonal antibody (M01), clone 2D5.Lane 1: ZNF101 transfected lysate(36.5 KDa).Lane 2: Non-transfected lysate.
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Immunohistochemistry-Paraffin: ZNF224 Antibody [NBP2-30794] - Staining of human skin shows moderate positivity in nuclear membrane in squamous epithelial cells.
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ZNF155 Antibody
NBP1-80912
ZNF155 Antibody
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Applications: WB, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol ZNF155
Entrez