ZNF10 Antibody - BSA Free

Western Blot: ZNF10 Antibody [NBP2-59679] - Total protein from human kidney, testis and Ntera2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ZNF10 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: ZNF10 Antibody - BSA Free [NBP2-59679] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with ZNF10 Antibody (NBP2-59679) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: ZNF10 Antibody [NBP2-59679] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human kidney tissue section with ZNF10 antibody at 1:500 dilution. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This ZNF10 antibody generated primarily a cytoplasmic staining with some nuclear positivity in all the cells of tubular epithelium. The staining was observed to be relatively stronger on the lumen side of the tubules. The cytoplasmic signal is potentially arising from the newly translated pool of this transcription factor.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ZNF10 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an internal sequence of the human Znf10 protein (between amino acids 200-250) [UniProt P21506].
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 - 10 ug/ml
  • Immunohistochemistry 2-4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Western Blot 1 - 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZNF10 Antibody - BSA Free

• KOX1
• KOX1zinc finger protein 10 (KOX 1)
• zinc finger protein 10
• Zinc finger protein KOX1
• ZNF10

Background

The protein encoded by this gene contains a C2H2 zinc finger, and has been shown to function as a transcriptional repressor. The Kruppel-associated box (KRAB) domain of this protein is found to be responsible for its transcriptional repression activity. RING finger containing protein TIF1 was reported to interact with the KRAB domain, and may serve as a mediator for the repression activity of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF10 Antibody (NBP2-59679)(1)

Publication using NBP2-59679

• Langini M Investigating the role of MALAT1, a long non-coding RNA, in glioblastoma tumorigenesis Thesis 2022-01-01 (WB, Human)
  
  Details:
  Dilution used 1:1000

Bioinformatics

• Gene Symbol: ZNF10