ZIC1 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

ZIC1 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human ZIC1
  Met1-Gly198
  Accession # Q15915
• Specificity: Detects human and mouse ZIC1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZIC1 Antibody [Alexa Fluor® 750]

• Zic family member 1 (odd-paired homolog, Drosophila)
• ZIC1
• ZICZic family member 1 (odd-paired Drosophila homolog)
• Zinc finger protein 201
• Zinc finger protein of the cerebellum 1
• ZNF201
• ZNF201zinc finger protein ZIC 1

Background

ZIC1 (zinc-finger protein of the cerebellum 1) is a 54-60 kDa member of the GLI C2H2-type Zn-finger protein family. It is expressed in embryonic sclerotome and dermomyotome and in postmitotic cerebellar granule cells and retinal ganglion cells. ZIC1 is a transcription factor that promotes ApoE expression and blocks activation of Math1, allowing for neuroblast multiplication at the expense of differentiation. Human ZIC1 is 447 amino acids (aa) in length. It contains N-terminal poly Ala and His segments, followed by five C2H2-type Zn-fingers. Although the first two are atypical (aa 225-296), the last three Zn-fingers (aa 302-384) are canonical and bind to GLI1, 2 and 3. There is one potential alternate start site at Met353. Over aa 1-198, human ZIC1 is 99% aa identical to mouse ZIC1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.