ZDHHC9 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ZDHHC9 Antibody [NBP1-84499] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.

Immunohistochemistry-Paraffin: ZDHHC9 Antibody [NBP1-84499] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Analysis in human cell lines A-431 and U2OS using Anti-ZDHHC9 antibody. Corresponding ZDHHC9 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ZDHHC9 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PSTQETSSSLLPQSPAPTEHLNSNEMPEDSSTPEEMPPPEPPEPPQEAAEAEK
• Predicted Species: Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZDHHC9
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ZDHHC9 Protein (NBP1-84499PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26493479).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ZDHHC9 Antibody - BSA Free

• CGI89
• CGI-89
• chromosome X open reading frame 11
• DHHC-9
• EC 2.3.1
• EC 2.3.1.-
• EC 2.3.1.12
• MRXSZ
• ZDHHC10
• Zinc finger DHHC domain-containing protein 9
• zinc finger, DHHC-type containing 10
• zinc finger, DHHC-type containing 9
• ZNF379MMSA1
• ZNF380DHHC domain containing 10

Background

The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZDHHC9 Antibody (NBP1-84499)(3)

Publications using NBP1-84499

• Kahlson M, Ritho J, Gomes J et al. Functional dissection of the zDHHC palmitoyltransferase 5–golgin A7 palmitoylation complex The Journal of Biological Chemistry 2025-10-01 [PMID: 40930250]
• Ko PJ, Woodrow C, Dubreuil MM et al. A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death Cell Chem Biol 2019-10-17 [PMID: 31631010]
• P Liu, B Jiao, R Zhang et al. Palmitoylacyltransferase Zdhhc9 inactivation mitigates leukemogenic potential of oncogenic Nras. Leukemia 2015-10-22 [PMID: 26493479] (WB, Mouse)

Bioinformatics

• Gene Symbol: ZDHHC9