ZCCHC11/TUT4 Antibody

Western Blot: ZCCHC11/TUT4 Antibody [NBP2-27539] - Analysis of Rat Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Approx. 170 kDa band observed in rat brain lysates (calculated MW of 163 kDa band according to rat NP_001101423.1).

• Reactivity: Mu, Rt
• Applications: WB, ELISA, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ZCCHC11/TUT4 Antibody Summary

• Immunogen: This ZCCHC11/TUT4 Antibody was developed against a peptide with sequence C-QKSAEKQGDLTPSET corresponding to internal region according to NP_780681.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TUT4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZCCHC11/TUT4 Antibody

• DKFZp779C1943
• EC 2.7.7.52
• KIAA0191FLJ42878
• PAP associated domain containing 3
• PAPD3
• terminal uridylyltransferase 4
• TUT4
• TUTase 4
• Zinc finger CCHC domain-containing protein 11
• zinc finger, CCHC domain containing 11

Background

Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZCCHC11/TUT4 Antibody (NBP2-27539)(1)

Publication using NBP2-27539

• Minoda Y, Saeki K, Aki D et al. A novel Zinc finger protein, ZCCHC11, interacts with TIFA andmodulates TLR signaling. Biochem Biophys Res Commun 2006-06-09 [PMID: 16643855]

Bioinformatics

• Gene Symbol: TUT4
• Entrez:
  • 230594(Mouse)
  • 313481(Rat)
• Uniprot:
  • NP_780681.2(Human)