![Western Blot: ZBTB4 Antibody [NBP3-18655] - Figure 2. Western blot analysis using the antibody directed against ZBTB4 Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against ZBTB4 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 105 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/ZBTB4-Antibody-Western-Blot-NBP3-18655-img0001.jpg "Western Blot: ZBTB4 Antibody [NBP3-18655] - Figure 2. Western blot analysis using the antibody directed against ZBTB4 Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against ZBTB4 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 105 kDa); the marker (in kDa) is shown on the left.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit ZBTB4 Antibody (NBP3-18655) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Polyclonal antibody raised in rabbit against human ZBTB4 (zinc finger and BTB domain containing 4), using four different KLH-conjugated synthetic peptides. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ZBTB4 |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![ELISA: ZBTB4 Antibody [NBP3-18655] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human ZBTB4 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:50,000.](http://images.novusbio.com/fullsize/ZBTB4 Antibody-ELISA-NBP3-18655-img0002.jpg "ELISA: ZBTB4 Antibody [NBP3-18655] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human ZBTB4 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:50,000.")
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