ZBED3 Antibody

Images

 
Western Blot: ZBED3 Antibody [NBP1-77348] - Analysis of ZBED3 in Jurkat cell lysate with ZBED3 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: ZBED3 Antibody [NBP1-77348] - A549 cells with ZBED3 antibody at 20 ug/ml.
Western Blot: ZBED3 Antibody [NBP1-77348] - Human lung tissue lysate with ZBED3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ZBED3 Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ZBED3. The immunogen is located within the last 50 amino acids of ZBED3.
Specificity
This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZBED3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ZBED3 Peptide (NBP1-77348PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZBED3 Antibody

  • BED domain containing 3
  • MGC15435,2610005H11Rik
  • zinc finger, BED-type containing 3

Background

Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ZBED3
Uniprot