ZBED3 Antibody

Western Blot: ZBED3 Antibody [NBP1-77348] - Analysis of ZBED3 in Jurkat cell lysate with ZBED3 antibody at 1 ug/ml.

Immunocytochemistry/Immunofluorescence: ZBED3 Antibody [NBP1-77348] - A549 cells with ZBED3 antibody at 20 ug/ml.

Product Discontinued

ZBED3 Antibody Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ZBED3. The immunogen is located within the last 50 amino acids of ZBED3. Amino Acid Squence: APPPLKDDPEGDRDG
• Specificity: This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZBED3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ZBED3 Peptide (NBP1-77348PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ZBED3 Antibody

• BED domain containing 3
• MGC15435,2610005H11Rik
• zinc finger, BED-type containing 3

Background

Axin is a key modulator of the Wnt/beta-catenin pathway and acts as a scaffold protein in phosphorylating and degrading cytoplasmic beta-catenin. ZBED3, a member of the Zinc finger BED-type domain containing superfamily, has been recently identified as an Axin-interacting protein and has been suggested to modulate the Wnt/beta-catenin signaling pathway. RNA interference (RNAi) experiments targeting ZBED3 RNA attenuated Wnt-induced beta-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and Wnt gene expression. Despite its predicted molecular weight, ZBED3 often migrates at a higher position in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ZBED1 or ZBED2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZBED3
• Uniprot:
  • NP_115743(Human)