Yes Antibody [Unconjugated]

Western blot shows lysates of L6 rat myoblast cell line, Rat-2 rat embryonic fibroblast cell line, MCF-7 human breast cancer cell line and, MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Yes was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Yes Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Yes
  Met1-Gly88
  Accession # P07947.3
• Specificity: Detects human and rat Yes.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: YES1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Yes Antibody [Unconjugated]

• cellular yes-1 protein
• c-yes
• EC 2.7.10
• EC 2.7.10.2
• HsT441
• P61-YES
• Proto-oncogene c-Yes
• proto-oncogene tyrosine-protein kinase Yes
• v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
• Yamaguchi sarcoma oncogene
• Yes
• YES1

Background

Yes is the cellular homolog of the oncogenic protein encoded by the Yamaguchi 73 and Esh avian sarcoma viruses. After Src, Yes is the most widely expressed member of the Src family of nonreceptor tyrosine kinases. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Yes Antibody (AF3205)(1)

Publication using AF3205

• William K. Kanagy, Cédric Cleyrat, Mohamadreza Fazel, Shayna R. Lucero, Marcel P. Bruchez, Keith A. Lidke et al. Docking of Syk to Fc epsilon RI is enhanced by Lyn but limited in duration by SHIP1 Molecular Biology of the Cell 2022-09-01 [PMID: 35793126]

Bioinformatics

• Gene Symbol: YES1
• Uniprot:
  • P07947.3()