YB1 Antibody (ST0432)

Western Blot: YB1 Antibody (ST0432) [NBP2-67491] - Analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat

Immunocytochemistry/Immunofluorescence: YB1 Antibody (ST0432) [NBP2-67491] - Staining YB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: YB1 Antibody (ST0432) [NBP2-67491] - Analysis of paraffin-embedded mouse stomach tissue using anti-YB1 antibody. Counter stained with hematoxylin.

Flow Cytometry: YB1 Antibody (ST0432) [NBP2-67491] - Analysis of Hela cells with YB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: YB1 Antibody (ST0432) [NBP2-67491] - Staining YB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: YB1 Antibody (ST0432) [NBP2-67491] - Staining YB1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: YB1 Antibody (ST0432) [NBP2-67491] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-YB1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: YB1 Antibody (ST0432) [NBP2-67491] - Analysis of paraffin-embedded human kidney tissue using anti-YB1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: YB1 Antibody (ST0432) [NBP2-67491] - Anaysis of paraffin-embedded mouse colon tissue using anti-YB1 antibody. Counter stained with hematoxylin.

Product Discontinued

YB1 Antibody (ST0432) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human YB1 aa 275-324 / 324. (SwissProt: P67809 Human; SwissProt: P62960 Mouse; SwissProt: P62961 Rat)
• Localization: Cytoplasm, Nucleus, Cytoplasmic granule, Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: YBX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for YB1 Antibody (ST0432)

• BP-8
• CBF-A
• class II, Y box-binding protein I
• CSDA2
• CSDB
• DBPB CCAAT-binding transcription factor I subunit A
• EFI-A
• Enhancer factor I subunit A
• MDR-NF1
• MGC104858
• MGC110976
• MGC117250
• NSEP1 Y-box-binding protein 1
• nuclease-sensitive element-binding protein 1
• Y box binding protein 1
• YB1 DNA-binding protein B
• YB-1 Y-box transcription factor
• YBX1

Background

The Y-box binding protein 1 (YB1) is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumor aggressiveness and belongs to the cold-shock domain family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for YB1 Antibody (NBP2-67491)(1)

Publication using NBP2-67491

• El-Ayoubi A, Klawitter M, Rüttinger J et al. Intranasal Delivery of the Oncolytic Adenovirus XVir-N-31 via Optimized Shuttle Cells Significantly Extends the Survival of Glioblastoma Bearing Mice preprints.org 2023-08-22 [PMID: 37894279] (IHC-Fr, Mouse)

Review for YB1 Antibody (NBP2-67491) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 06/12/2023

Summary

• Application: Flow Cytometry
• Sample Tested: Thymocytes
• Species: Mouse
• Lot: HK0804

Comments

• Comments: Staining after fixation with the Foxp3 Staining Buffer Set worked nicely.

Bioinformatics

• Gene Symbol: YBX1