This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Full Length Recombinant Protein
Gene
YAP1
Purity
>80% by SDS-PAGE and Coomassie blue staining
Applications/Dilutions
Dilutions
ELISA
Functional
Immunoaffinity Purification
Immunocytochemistry/Immunofluorescence
In vitro assay
Protein Array
Western Blot
Application Notes
Use in In vitro reported in scientific literature (PMID:33297404).
Theoretical MW
80.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4 using H00010413-P01 in the following applications:
Publications
Read Publications using H00010413-P01 in the following applications:
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Recombinant Human YAP1 GST (N-Term) Protein
65 kDa Yes-associated protein
COB1
Protein Yorkie Homolog
Transcriptional Coactivator YAP1
YAP
YAP1
YAP1-2gamma
YAP2
YAP2L
YAP65
YAP65yes-associated protein 2
Yes Associated Protein
Yes-associated protein 1
Yes-associated protein 1, 65kDa
yes-associated protein beta
yes-associated protein delta
Yes-Associated Protein YAP65 Homolog
Yes-Associated Protein
YKI
Yorkie Homolog
Background
The transcriptional coactivator Yes-associated protein (YAP), also known as Yes-associated protein 1 (YAP1), Yes-Associated Protein YAP65 Homolog, Protein Yorkie Homolog and YAP65 has a theoretical molecular weight of 65 kDa. The YAP1 gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 shares homology with the WW domain of transcriptional co-activator with PDZ-binding motif (TAZ), which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors and is likely involved in protein-protein interaction. YAP has been identified as a core regulatory mechanism that blocks mammalian glial cell proliferation and cellular reprogramming following damage (1).
YAP plays a role in the development and progression of multiple cancers as a transcriptional regulator of the Hippo signaling pathway. YAP1 encodes a nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis to play a pivotal role in controlling cell growth and organ size and has emerged as a key player in tumor suppression (2,3). Deregulation of the Hippo pathway causes tumor formation and malignancy, with YAP being a key oncogenic driver in liver carcinogenesis (2) and may function as a potential target for cancer treatment (3).
References
1. Rueda, E. M., Hall, B. M., Hill, M. C., Swinton, P. G., Tong, X., Martin, J. F., & Poche, R. A. (2019). The Hippo Pathway Blocks Mammalian Retinal Muller Glial Cell Reprogramming. Cell Rep, 27(6), 1637-1649.e1636. doi:10.1016/j.celrep.2019.04.047
2. Liu, A. M., Xu, M. Z., Chen, J., Poon, R. T., & Luk, J. M. (2010). Targeting YAP and Hippo signaling pathway in liver cancer. Expert Opin Ther Targets, 14(8), 855-868. doi:10.1517/14728222.2010.499361
3.Ye, S., & Eisinger-Mathason, T. S. (2016). Targeting the Hippo pathway: Clinical implications and therapeutics. Pharmacol Res, 103, 270-278. doi:10.1016/j.phrs.2015.11.025
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
Bioinformatics Tool for YAP1 Recombinant Protein (H00010413-P01)
Discover related pathways, diseases and genes to YAP1 Recombinant Protein (H00010413-P01). Need help?
Read the Bioinformatics Tool Guide for instructions on using this tool.
Diseases for YAP1 Recombinant Protein (H00010413-P01)
Discover more about diseases related to YAP1 Recombinant Protein (H00010413-P01).
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.