YAP1 Antibody (867711) [Alexa Fluor® 647]

• Applications: WB, IHC, CyTOF-ready, KO
• Clone: 867711
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

YAP1 Antibody (867711) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human YAP1
  Ala347-Leu504
  Accession # P46937
• Specificity: Detects human YAP1 in ELISA. Detects human, mouse and rat YAP1 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

YAP1 (Yes-associated protein 1), also called YAP65 or Yorkie Homolog, is a widely expressed 65 kDa cytoplasmic and nuclear transcriptional regulator protein in the Hippo pathway that regulates organ size. The 504 amino acid (aa) human YAP1 contains two WW (tryptophan-containing protein binding) domains (aa 171-263), a transactivation domain (aa 219-504) and 19 sites that may be phosphorylated by kinases such as LATS1 and 2, MAPK8 and 9, CK1, or ABL1. Isoforms of 488, 454 and 326 aa lack aa 328-343, 230-267 (plus a 3 aa substitution for aa 329-343), or 1-178, respectively. YAP1 binds the Yes tyrosine kinase SH3 domain and many other proteins, notably ErbB4. YAP1 expression is increased in some liver and prostate cancers. Within aa 347-504, human YAP1 shares 94% and 93% aa sequence identity with mouse and rat YAP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.