XBP1 Antibody

Images

 
Western Blot: XBP1 Antibody [NB100-80861] - XBP-1 in PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody [NB100-80861] - XBP-1 in HepG2 cells with XBP-1 antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: XBP1 Antibody [NB100-80861] - Staining of human spleen. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunohistochemistry-Paraffin: XBP1 Antibody [NB100-80861] - Staining of human kidney. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

XBP1 Antibody Summary

Immunogen
Synthetic peptide from human XBP1.
Specificity
17 amino acid peptide from near the carboxy terminus of human XBP-1. This antibody will only recognize the non-spliced (shorter) isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Control
XBP1 Lysate (NBL1-17900)
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-80861 in the following applications:

Publications
Read Publications using
NB100-80861 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XBP1 Antibody

  • HTF
  • TREB5
  • X-box binding protein 1
  • X-box-binding protein 1
  • XBP1
  • XBP-1
  • XBP2
  • XBP2Tax-responsive element-binding protein 5

Background

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. The protein contains two domains commonly found in transcription factors that confer DNA binding and dimerization capability. This gene product was also separately identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. There is also a recent finding that the same agents that cause an ER stress response, the unfolded protein response, also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P

Publications for XBP1 Antibody (NB100-80861)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Reviews for XBP1 Antibody (NB100-80861) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB100-80861:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot XBP1 NB100-80861
Enlarge
reviewed by:
Frank Culbertson
WB Mouse 05/05/2017
View

Summary

ApplicationWestern Blot
Sample Tested Mouse brain
SpeciesMouse
Western Blot XBP1 NB100-80861
Enlarge
reviewed by:
Anonymous
WB Mouse 03/16/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain
SpeciesMouse
Lot22855

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XBP1 Antibody (NB100-80861). (Showing 1 - 1 of 1 FAQs).

  1. I learn from your company web that you are providing atf6, xbp1, cbx4 antibodies against human for immunofluorescence. And from the provided pictures, it seems very good. But I am wondering if anyone use them for immunofluorescence in human cells in your memory or in published papers? And how about them? By the way, you are providing free sample size for the atf6, xbp1 antibodies, aren't you? Could you send me some for tests? One more question, do they work on endogenous proteins but not only on overexpressed proteins?
    • These products have been validated for use in IF on human: catalog numbers NBP1-40256, NB100-80861, and NBP1-83225. Yes these antibodies should work on the native protein/endogenous protein.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for XBP1 Antibody (NB100-80861)

Discover related pathways, diseases and genes to XBP1 Antibody (NB100-80861). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for XBP1 Antibody (NB100-80861)

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PTMs for XBP1 Antibody (NB100-80861)

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Blogs on XBP1.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

IRE1 - an important sensor in the unfolded protein response pathway
During cellular stress the protein folding capacity of the ER is diminished. In order to maintain homeostasis and ensure proper protein folding cells activate the unfolded protein response (UPR), a signaling network consisting of sensors and effect...  Read full blog post.

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Frank Culbertson
05/05/2017
Application: WB
Species: Mouse

Anonymous
03/16/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol XBP1
Entrez
Uniprot