XBP1 Antibody

Western Blot: XBP1 Antibody [NB100-80861] - XBP-1 in PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NB100-80861] - XBP-1 in HepG2 cells with XBP-1 antibody at 2 ug/ml.

Immunohistochemistry-Paraffin: XBP1 Antibody [NB100-80861] - Staining of human spleen. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry-Paraffin: XBP1 Antibody [NB100-80861] - Staining of human kidney. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

XBP1 Antibody Summary

• Immunogen: A 17 aa synthetic peptide near the C-terminus of human XBP1. The immunogen is located within the last 50 aa of XBP1.
• Epitope: Near the C-Terminus
• Specificity: 17 amino acid peptide from near the carboxy terminus of human XBP1. This antibody will only recognize the non-spliced (shorter) isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XBP1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Control:

  XBP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Peptide affinity purified

Alternate Names for XBP1 Antibody

• HTF
• TREB5
• X-box binding protein 1
• X-box-binding protein 1
• XBP1
• XBP-1
• XBP2
• XBP2Tax-responsive element-binding protein 5

Background

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. The protein contains two domains commonly found in transcription factors that confer DNA binding and dimerization capability. This gene product was also separately identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. There is also a recent finding that the same agents that cause an ER stress response, the unfolded protein response, also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XBP1 Antibody (NB100-80861)(3)

Publications using NB100-80861

• Benedetti R, Gilardini Montani MS, Romeo MA et al. Role of UPR Sensor Activation in Cell Death-Survival Decision of Colon Cancer Cells Stressed by DPE Treatment Biomedicines 2021-09-18 [PMID: 34572447] (WB, Human)
• Tong J, Huang C, Bi F et al. XBP1 Depletion Precedes Ubiquitin Aggregation and Golgi Fragmentation in TDP-43 Transgenic Rats. J Neurochem. 2012-11-01 [PMID: 22970712] (WB, Rat)
• Baek HA, Kim do S, Park HS et al. Involvement of endoplasmic reticulum stress in myofibroblastic differentiation of lung fibroblasts Am J Respir Cell Mol Biol 2012-06-01 [PMID: 21852685]

Reviews for XBP1 Antibody (NB100-80861) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Frank Culbertson WB Mouse 05/05/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse brain
• Species: Mouse

reviewed by: Verified Customer WB Mouse 03/16/2017

Summary

• Application: Western Blot
• Sample Tested: Brain
• Species: Mouse
• Lot: 22855

FAQs for XBP1 Antibody (NB100-80861). (Showing 1 - 1 of 1 FAQs).

1. I learn from your company web that you are providing atf6, xbp1, cbx4 antibodies against human for immunofluorescence. And from the provided pictures, it seems very good. But I am wondering if anyone use them for immunofluorescence in human cells in your memory or in published papers? And how about them? By the way, you are providing free sample size for the atf6, xbp1 antibodies, aren't you? Could you send me some for tests? One more question, do they work on endogenous proteins but not only on overexpressed proteins?
   • These products have been validated for use in IF on human: catalog numbers NBP1-40256, NB100-80861, and NBP1-83225. Yes these antibodies should work on the native protein/endogenous protein.

Bioinformatics

• Gene Symbol: XBP1
• Entrez:
  • 7494(Human)
  • 22433(Mouse)
  • 289754(Rat)
• Uniprot:
  • P17861(Human)