XBP1 Antibody (JM10-62)

Western Blot: XBP1 Antibody (JM10-62) [NBP2-66789] - Analysis of XBP1 on different cell lysates using anti-XBP1 antibody at 1/1,000 dilution. Positive control: Lane 1:HepG2 Lane 2: 293T

Immunocytochemistry/Immunofluorescence: XBP1 Antibody (JM10-62) [NBP2-66789] - Staining XBP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: XBP1 Antibody (JM10-62) [NBP2-66789] - Analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody. Counter stained with hematoxylin.

Flow Cytometry: XBP1 Antibody (JM10-62) [NBP2-66789] - Analysis of 293T cells with XBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: XBP1 Antibody (JM10-62) [NBP2-66789] - Staining XBP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: XBP1 Antibody (JM10-62) [NBP2-66789] - Staining XBP1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: XBP1 Antibody (JM10-62) [NBP2-66789] - Analysis of paraffin-embedded human colon cancer tissue using anti-XBP1 antibody. Counter stained with hematoxylin.

Product Discontinued

XBP1 Antibody (JM10-62) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human XBP1 aa 126-175 / 261. (SwissProt: P17861 Human)
• Localization: Nucleus, Chromosome, Chromosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: XBP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for XBP1 Antibody (JM10-62)

• HTF
• TREB5
• X-box binding protein 1
• X-box-binding protein 1
• XBP1
• XBP-1
• XBP2
• XBP2Tax-responsive element-binding protein 5

Background

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. The protein contains two domains commonly found in transcription factors that confer DNA binding and dimerization capability. This gene product was also separately identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. There is also a recent finding that the same agents that cause an ER stress response, the unfolded protein response, also increase transcription of this gene. This increase in transcription requires an ER stress response consensus binding element in the promoter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XBP1