XAB1 Antibody (GPN1/2350)

Western Blot: XAB1 Antibody (GPN1/2350) [NBP2-75782] - Analysis of human Brain tissue lysate using XAB1 Antibody (GPN1/2350).

Immunohistochemistry-Paraffin: XAB1 Antibody (GPN1/2350) [NBP2-75782] - Formalin-fixed, paraffin-embedded human Pancreas stained with GPN1 Mouse Monoclonal Antibody (GPN1/2350).

Protein Array: XAB1 Antibody (GPN1/2350) [NBP2-75782] - Analysis of Protein Array containing more than 19,000 full-length human proteins using XAB1 Antibody (GPN1/2350) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IHC-P, PA
• Clone: GPN1/2350
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

XAB1 Antibody (GPN1/2350) Summary

• Immunogen: Recombinant full-length human XAB1 protein
• Localization: Cytoplasmic & Nuclear
• Specificity: GPN1 is involved in protein synthesis events. It is expressed ubiquitously with highest expression in testis. It binds to the RNA polymerase II- (Poll II) associated proteins RPAP1-3 and to XPA (a protein involved in DNA repair mechanisms), thereby forming an interface with Poll II. Via this interaction, GPN1 is thought to mediate the involvement of Pol II in both protein complex formation and protein chaperone/ scaffolding activities. In addition, GPN1 interacts with components of the integrator and molecular chaperone complexes, further implicating GPN1 in protein assembly. GPN1 contains a cluster of acidic amino acids in its C-terminal region and a series of sequences similar to those found in GTP-binding proteins in its N-terminal region, suggesting that GPN1 has possible GTPase activity.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: Positive Control: Pancreas, Testis, Thyroid or Bladder. ELISA: For coating, order BSA free version of this antibody. Immunohistochemistry-Paraffin: 1-2 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for XAB1 Antibody (GPN1/2350)

• ATP(GTP)-binding protein
• ATPBD1A
• GPN-loop GTPase 1
• GTPase
• MBD2-interacting protein
• MBDin
• MBDINXPA binding protein 1
• XAB1FLJ51176
• XPA-binding protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPN1
• Uniprot:
  • Q9HCN4(Human)