XAB1 Antibody (GPN1/2350)

Images

 
Western Blot: XAB1 Antibody (GPN1/2350) [NBP2-75782] - Analysis of human Brain tissue lysate using XAB1 Antibody (GPN1/2350).
Immunohistochemistry-Paraffin: XAB1 Antibody (GPN1/2350) [NBP2-75782] - Formalin-fixed, paraffin-embedded human Pancreas stained with GPN1 Mouse Monoclonal Antibody (GPN1/2350).
Protein Array: XAB1 Antibody (GPN1/2350) [NBP2-75782] - Analysis of Protein Array containing more than 19,000 full-length human proteins using XAB1 Antibody (GPN1/2350) Z- and S- Score: The Z-score represents the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, PA
Clone
GPN1/2350
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

XAB1 Antibody (GPN1/2350) Summary

Immunogen
Recombinant full-length human XAB1 protein
Localization
Cytoplasmic & Nuclear
Specificity
GPN1 is involved in protein synthesis events. It is expressed ubiquitously with highest expression in testis. It binds to the RNA polymerase II- (Poll II) associated proteins RPAP1-3 and to XPA (a protein involved in DNA repair mechanisms), thereby forming an interface with Poll II. Via this interaction, GPN1 is thought to mediate the involvement of Pol II in both protein complex formation and protein chaperone/ scaffolding activities. In addition, GPN1 interacts with components of the integrator and molecular chaperone complexes, further implicating GPN1 in protein assembly. GPN1 contains a cluster of acidic amino acids in its C-terminal region and a series of sequences similar to those found in GTP-binding proteins in its N-terminal region, suggesting that GPN1 has possible GTPase activity.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GPN1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Positive Control: Pancreas, Testis, Thyroid or Bladder. ELISA: For coating, order BSA free version of this antibody. Immunohistochemistry-Paraffin: 1-2 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for XAB1 Antibody (GPN1/2350)

  • ATP(GTP)-binding protein
  • ATPBD1A
  • GPN-loop GTPase 1
  • GTPase
  • MBD2-interacting protein
  • MBDin
  • MBDINXPA binding protein 1
  • XAB1FLJ51176
  • XPA-binding protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75783AF350
Alexa Fluor 405 NBP2-75783AF405
Alexa Fluor 488 NBP2-75783AF488
Alexa Fluor 532 NBP2-75783AF532
Alexa Fluor 594 NBP2-75783AF594
Alexa Fluor 647 NBP2-75783AF647
Alexa Fluor 700 NBP2-75783AF700
Alexa Fluor 750 NBP2-75783AF750
Biotin NBP2-75783B
DyLight 350 NBP2-75783UV
DyLight 405 NBP2-75783V
DyLight 488 NBP2-75783G
DyLight 550 NBP2-75783R
DyLight 594 NBP2-75783DL594
DyLight 650 NBP2-75783C
DyLight 680 NBP2-75783FR
DyLight 755 NBP2-75783IR
FITC NBP2-75783F
HRP NBP2-75783H
Janelia Fluor 549 NBP2-75783JF549
Janelia Fluor 646 NBP2-75783JF646

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Bioinformatics

Gene Symbol GPN1
Uniprot