WSTF Antibody (5E9)

Immunohistochemistry-Paraffin: WSTF Antibody (5E9) [H00009031-M01] - Analysis of monoclonal antibody to BAZ1B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.

Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01] - Analysis of protein-protein interactions between SMARCB1 and BAZ1B. HeLa cells were stained with anti-SMARCB1 rabbit purified polyclonal 1:1200 and anti-BAZ1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: WSTF Antibody (5E9) [H00009031-M01] - Analysis of protein-protein interactions between SMARCB1 and BAZ1B. Huh7 cells were stained with anti-SMARCB1 rabbit purified polyclonal 1:1200 and anti-BAZ1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: WSTF Antibody (5E9) [H00009031-M01] - Detection limit for recombinant GST tagged BAZ1B is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: ELISA, IHC, IHC-P, PLA, S-ELISA
• Clone: 5E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

WSTF Antibody (5E9) Summary

• Immunogen: BAZ1B (NP_075381, 1384 a.a. ~ 1483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK
• Localization: Nuclear
• Specificity: BAZ1B - bromodomain adjacent to zinc finger domain, 1B
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BAZ1B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Sandwich ELISA
• Application Notes: Antibody reactivity against recombinant protein on ELISA. It has been used for IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for WSTF Antibody (5E9)

• bromodomain adjacent to zinc finger domain, 1B
• williams-Beuren syndrome chromosomal region 10 protein

Background

WSTF (Williams syndrome transcription factor) is a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. WSTF forms a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. This gene is deleted in Williams-Beuren syndrome, a developmental disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAZ1B
• Entrez:
  • 9031(Human)
• OMIM:
  • 605681(Human)