Wnt3 Antibody (OTI1G7) - Azide and BSA Free

Images

 
Western Blot: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3.
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI1G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Wnt3 Antibody (OTI1G7) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 128-355 of human WNT3 (NP_110380) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
WNT3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunohistochemistry 1:150
Theoretical MW
37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for Wnt3 Antibody (OTI1G7) - Azide and BSA Free

  • INT4Proto-oncogene Int-4 homolog
  • MGC131950
  • MGC138321
  • MGC138323
  • proto-oncogene Wnt-3
  • wingless-type MMTV integration site family, member 3
  • WNT-3 proto-oncogene protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74869AF350
Alexa Fluor 405 NBP2-74869AF405
Alexa Fluor 488 NBP2-74869AF488
Alexa Fluor 532 NBP2-74869AF532
Alexa Fluor 594 NBP2-74869AF594
Alexa Fluor 647 NBP2-74869AF647
Alexa Fluor 700 NBP2-74869AF700
Alexa Fluor 750 NBP2-74869AF750
Biotin NBP2-74869B
DyLight 350 NBP2-74869UV
DyLight 405 NBP2-74869V
DyLight 488 NBP2-74869G
DyLight 550 NBP2-74869R
DyLight 594 NBP2-74869DL594
DyLight 650 NBP2-74869C
DyLight 680 NBP2-74869FR
DyLight 755 NBP2-74869IR
FITC NBP2-74869F
HRP NBP2-74869H
Janelia Fluor 549 NBP2-74869JF549
Janelia Fluor 646 NBP2-74869JF646

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Contact Information

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Bioinformatics

Gene Symbol WNT3