Wnt-8a Antibody (HL1648) - Azide and BSA Free

Immunohistochemistry-Paraffin: Wnt-8a Antibody (HL1648) - Azide and BSA Free [NBP3-25755] - Wnt8a antibody [HL1648] detects Wnt8a protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Wnt8a stained by Wnt8a antibody [HL1648] (NBP3-25755) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Wnt-8a Antibody (HL1648) - Azide and BSA Free [NBP3-25755] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (NBP3-25755) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Wnt-8a Antibody (HL1648) - Azide and BSA Free [NBP3-25755] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (NBP3-25755) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Wnt-8a antibody [HL1648] detects Wnt-8a protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Wnt-8a stained by Wnt-8a antibody [HL1648] (NBP3-25755) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10 um.

• Reactivity: Hu, Ze
• Applications: WB, IHC
• Clone: HL1648
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Wnt-8a Antibody (HL1648) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Wnt-8a Antibody (HL1648) - Azide and BSA Free (NBP3-25755) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Wnt-8a
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WNT8A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Wnt-8a Antibody (HL1648) - Azide and BSA Free

• protein Wnt-8a
• Protein Wnt-8d
• wingless-type MMTV integration site family, member 8A
• Wnt8a
• Wnt-8a
• WNT8DWNT8d

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. It encodes a protein which shows 81% amino acid identity to the mouse Wnt8A protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT8A