Wnt-7b Antibody [Unconjugated]

Wnt‑7b was detected in immersion fixed paraffin-embedded sections of human breast cancer using Goat Anti-Human Wnt‑7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-7b Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Wnt‑7b
  Leu32-Glu72 & Thr216-Ala283
  Accession # P56706
• Specificity: Detects human Wnt-7b in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WNT7B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-7b Antibody [Unconjugated]

• protein Wnt-7b
• wingless-type MMTV integration site family, member 7B
• Wnt7b
• Wnt-7b

Background

Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid-modified and are ligands for members of the frizzled family of receptors, which mediates cell-cell communication during development. Human Wnt-7b is synthesized as a 349 amino acid (aa) precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Mature human Wnt-7b shares 99% aa sequence identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa sequence identity with Wnt-7a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-7b Antibody (AF3460)(16)

Publications using AF3460

• Martin Flores, N;Podpolny, M;McLeod, F;Workman, I;Crawford, K;Ivanov, D;Leonenko, G;Escott-Price, V;Salinas, PC; Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer's disease, restores synapse integrity and memory in a disease mouse model eLife 2024-01-29 [PMID: 38285009] (Immunohistochemistry, Mouse)
• Militi, S;Nibhani, R;Jalali, M;Pauklin, S; RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling Cell reports 2023-09-18 [PMID: 37725511] (ICC, Human)
• J Seong, J Frias-Alde, V Holzmann, H Kagawa, G Sestini, H Heidari Kh, Y Scholte Op, M Kip, SJ Pradhan, L Verwegen, J Vivié, L Li, A Alemany, J Korving, F Darmis, A van Oudena, D Ten Berge, N Geijsen, NC Rivron Epiblast inducers capture mouse trophectoderm stem cells in�vitro and pattern blastoids for implantation in utero Cell Stem Cell, 2022-07-07;29(7):1102-1118.e8. 2022-07-07 [PMID: 35803228] (Western Blot, Mouse)
• Z Zhang, Y Xu, C Zhao Fzd7/Wnt7b signaling contributes to stemness and chemoresistance in pancreatic cancer Cancer Medicine, 2021-05-02;10(10):3332-3345. 2021-05-02 [PMID: 33934523] (IHC, Western Blot, Human)
• Shuai Jiang, Qiwen Li, Yimin Liu, Huimin Zhang, Qianyu Wang, Yu Chen et al. Activation of WNT7b autocrine eases metastasis of colorectal cancer via epithelial to mesenchymal transition and predicts poor prognosis BMC Cancer 2021-12-01 [PMID: 33607955]
• Z Wang, P Yin, Y Sun, L Na, J Gao, W Wang, C Zhao LGR4 maintains HGSOC cell epithelial phenotype and stem-like traits Gynecol Oncol, 2020-09-24;0(0):. 2020-09-24 [PMID: 32980127] (Western Blot, Human)
• JS Beeler, CB Marshall, PI Gonzalez-E, TM Shaver, GL Santos Gua, ST Lea, KN Johnson, H Jin, BJ Venters, ME Sanders, JA Pietenpol p73 regulates epidermal wound healing and induced keratinocyte programming PLoS ONE, 2019-06-19;14(6):e0218458. 2019-06-19 [PMID: 31216312] (Western Blot, Human, Mouse)
• Thomas Volckaert, Tingting Yuan, Cho-Ming Chao, Harold Bell, Alina Sitaula, Luisa Szimmtenings et al. Fgf10-Hippo Epithelial-Mesenchymal Crosstalk Maintains and Recruits Lung Basal Stem Cells Developmental Cell 2017-10-01 [PMID: 29017029]
• M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling Respir. Res, 2017-03-24;18(1):52. 2017-03-24 [PMID: 28340578] (IHC-Fr, Human)
• Bruce F Kimler WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation J. Endocrinol., 2016-03-14;229(2):197-207. 2016-03-14 [PMID: 26975616] (IHC-P, Rat)
• Donna R. Newman, W. Shane Sills, Katherine Hanrahan, Amanda Ziegler, Kathleen McGinnis Tidd, Elizabeth Cook et al. Expression of WNT5A in Idiopathic Pulmonary Fibrosis and Its Control by TGF-beta and WNT7B in Human Lung Fibroblasts Journal of Histochemistry & Cytochemistry 2016-02-01 [PMID: 26538547] (Western Blot, Human)
• Shiah S, Hsiao J, Chang W, Chen Y, Jin Y, Wong T, Huang J, Tsai S, Hsu Y, Chou S, Yen Y, Jiang S, Shieh Y, Chang I, Hsiao M, Chang J Downregulated miR329 and miR410 promote the proliferation and invasion of oral squamous cell carcinoma by targeting Wnt-7b. Cancer Res, 2014-10-28;74(24):7560-72. 2014-10-28 [PMID: 25351956] (IHC, Human)
• Dali Zheng, Keith F. Decker, Tianhua Zhou, Jianquan Chen, Zongtai Qi, Kathryn Jacobs et al. Role of WNT7B-induced Noncanonical Pathway in Advanced Prostate Cancer Molecular Cancer Research 2013-05-01 [PMID: 23386686]
• Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells. Int J Oncol, 2012-10-17;41(6):2021-8. 2012-10-17 [PMID: 23076981] (Western Blot, Human)
• Volckaert T, Dill E, Campbell A, Tiozzo C, Majka S, Bellusci S, De Langhe SP Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. J. Clin. Invest., 2011-10-10;121(11):4409-19. 2011-10-10 [PMID: 21985786] (IHC-P, Mouse)
• Gogolla N, Galimberti I, Deguchi Y, Caroni P Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus. Neuron, 2009-05-28;62(4):510-25. 2009-05-28 [PMID: 19477153] (IHC-Fr, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: WNT7B
• Uniprot:
  • P56706()